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81.
Daniel D. Warnock Johannes Lehmann Thomas W. Kuyper Matthias C. Rillig 《Plant and Soil》2007,300(1-2):9-20
Experiments suggest that biomass-derived black carbon (biochar) affects microbial populations and soil biogeochemistry. Both
biochar and mycorrhizal associations, ubiquitous symbioses in terrestrial ecosystems, are potentially important in various
ecosystem services provided by soils, contributing to sustainable plant production, ecosystem restoration, and soil carbon
sequestration and hence mitigation of global climate change. As both biochar and mycorrhizal associations are subject to management,
understanding and exploiting interactions between them could be advantageous. Here we focus on biochar effects on mycorrhizal
associations. After reviewing the experimental evidence for such effects, we critically examine hypotheses pertaining to four
mechanisms by which biochar could influence mycorrhizal abundance and/or functioning. These mechanisms are (in decreasing
order of currently available evidence supporting them): (a) alteration of soil physico-chemical properties; (b) indirect effects
on mycorrhizae through effects on other soil microbes; (c) plant–fungus signaling interference and detoxification of allelochemicals
on biochar; and (d) provision of refugia from fungal grazers. We provide a roadmap for research aimed at testing these mechanistic
hypotheses. 相似文献
82.
83.
An alkaline nuclease was purified from microplasmodia of Physarum polycephalum. The nuclease, active on denatured DNA and RNA and free of contamination by other nucleolytic activities, appeared to be a zinc-metallo protein. The enzyme was only active under conditions, where Zn2+ were retained in the enzyme. Loss of zinc occurred by the chelating action of EDTA, EGTA or ampholines, by acid of highly alkaline pH conditions or by high ionic strength. The addition of ZnCl2 to compensate losses, restored all activity, while all other divalent cations caused inhibition. The nuclease, with a molecular weight of 32 000, was stable at neutral pH at high temperatures with a half-life of 20 min at 80 degrees C. It was inhibited by any salt of buffer concentration above the level of zero ionic strength and showed a special sensitivity towards phosphate ions. The possible similarity of this enzyme to nuclease S1 from Aspergillus oryzae is pointed out. 相似文献
84.
T L Rutten T H van Kuppevelt H M Janssen C M Kuyper 《European journal of cell biology》1988,45(2):256-261
By use of the cationic dye Cuprolinic Blue in a critical electrolyte concentration method, the lungs of mice ranging from the late fetal stage (17 days of gestation) to the puberal stage (27 days) were surveyed for their proteoglycans. A large Cuprolinic Blue-positive filament is present within the connective tissue of lungs of late fetal and young postnatal mice. It is mostly located at the boundary between large extracellular matrix structures and electron microscopically empty areas, but sometimes also at the surface of fibroblast-like cells. The stainability of the filament disappears after treatment with chondroitinase ABC or chondroitinase AC, but not after treatment with nitrous acid. The Cuprolinic Blue-positive structure appears to be most abundant around 2 days postnatally. From day 10 on, its number decreases dramatically, and it can be no longer observed in the lungs of 27-day-old mice. 相似文献
85.
T. W. Kuyper 《The New phytologist》2008,177(2):297-300
86.
T. H. M. S. M. Van Kuppevelt F. P. M. Cremers J. G. W. Domen C. M. A. Kuyper 《The Histochemical journal》1984,16(6):671-686
Summary The nature of Cuprolinic Blue-positive anionic filaments in mouse lung alveoli has been characterized. The contrast of filaments in the alveolar basement membrane of type I epithelial cells was lost on treatment with nitrous acid and pronase (without prefixation). In contrast, neither neuraminidase, chondroitinase ABC or AC, norStreptomyces hyaluronidase had any effect. Treatment with pronase (after prefixation) and 2.0m MgCl2 (after prefixation) also had no effect, indicating that the filaments are heparan sulphate proteoglycans. The filaments in the alveolar basement membrane of type II epithelial cells and in the capillary basement membrane of the endothelial cells were also nitrous acid sensitive, but chondroitinase ABC-insensitive. A model in which the whole alveolus contains a single layer of heparan sulphate-containing proteoglycan monomers is proposed. Furthermore, the collagen fibril associated filaments remained unaffected after treatment with nitrous acid, neuraminidase orStreptomyces hyaluronidase, or after digestion with pronase (after prefixation) and treatment with 2.0m MgCl2 (after prefixation). These filaments, however, could no longer be detected when digestion with chondroitinase ABC or pronase (without prefixation) was applied; chondroitinase AC treatment clearly affected the filaments, although they still were visible. These results indicate that the filaments are dermatan sulphate-containing proteoglycans. Some functional aspects of the proteoglycans are discussed. 相似文献
87.
F. J. M. Verhagen H. J. Swarts T. W. Kuyper J. B. P. A. Wijnberg J. A. Field 《Applied microbiology and biotechnology》1996,45(5):710-718
Recently, several species of basidiomycetes were shown to produce de novo high concentrations of chloroaromatic metabolites. Since these lignocellulose-degrading fungi play a major role in the ecosphere,
the purpose of this study was to determine the ubiquity of organohalogen production among basidiomycetes. A total of 191 fungal
strains were monitored for adsorbable organic halogen (AOX) production when grown on defined liquid media. Approximately 50%
of the strains tested and 55% of the genera tested produced AOX. A low production of 0.1–0.5 mg AOX/l was observed among 25%
of the strains, a moderate production of 0.5–5.0 mg AOX/l was observed among 16% of the strains and 9% of the strains produced
high levels (5–67 mg AOX/l). The latter group was dominated by species belonging to the genera Hypholoma, Mycena and Bjerkandera, showing specific AOX productions in the range 1074–30893 mg AOX/kg dry weight of mycelial biomass. Many highly ecologically
significant fungal species were identified among the moderate to high producers. These species were also able to produce AOX
when cultivated on natural lignocellulosic substrates. Hypholoma fasciculare and Mycena metata respectively produced up to 132 mg and 193 mg AOX/kg dry weight of forest litter substrate in 6 weeks.
Received: 5 October 1995/Received revision: 28 December 1995/Accepted: 12 February 1996 相似文献
88.
T H van Kuppevelt H M van Beuningen T L Rutten A J van den Brule C M Kuyper 《European journal of cell biology》1986,39(2):386-390
By use of the cationic dye Cuprolinic Blue in a critical electrolyte concentration method, heavily staining, generally large, filaments have been demonstrated in human lung alveoli. In some lung specimens they are abundant, while in others they are very scanty. The filaments are seen: around bundles of collagen fibrils, at places which seem electron microscopically almost empty, associated with basement membranes around elastin, and sometimes associated with individual collagen fibrils. After poststaining tiny threads--connecting the filaments--could sometimes be observed. The filaments are resistant to treatment with nitrous acid, heparitinase or pronase after prefixation. After digestion with chondroitinase ABC, chondroitinase AC or pronase without prefixation, the filaments are no longer detectable. The tiny threads are chondroitinase ABC resistant. It is concluded that the Cuprolinic Blue-positive filaments represent proteoglycans which contain chondroitin sulfate and/or glucuronic acid-rich dermatan sulfate. The possible role of these proteoglycans in tissue repair is discussed. 相似文献
89.