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41.
The present paper describes and discusses the seasonal cycle of primary production, standing-stock, climatological and environmental factors, and their interrelationships in a mangrove swamp of the estuarine type (25°S, South Latitude, Cananéia, Brasil). Comparisons with values of primary production for other tropical and temperate environments have been made. Further lines of work are indicated. 相似文献
42.
GW Patton R Stephens IA Sidorov X Xiao RA Lempicki DS Dimitrov RH Shoemaker G Tudor 《BMC bioinformatics》2006,7(1):81
Background
Microarrays used for gene expression studies yield large amounts of data. The processing of such data typically leads to lists of differentially-regulated genes. A common terminal data analysis step is to map pathways of potentially interrelated genes. 相似文献43.
44.
RH Behrens Z Bisoffi A Björkman J Gascon C Hatz T Jelinek F Legros N Mühlberger P Voltersvik 《Malaria journal》2006,5(1):1-4
Background
Thick blood films are routinely used to diagnose Plasmodium falciparum malaria. Here, they were used to diagnose volunteers exposed to experimental malaria challenge.Methods
The frequency with which blood films were positive at given parasite densities measured by PCR were analysed. The poisson distribution was used to calculate the theoretical likelihood of diagnosis. Further in vitro studies used serial dilutions to prepare thick films from malaria cultures at known parasitaemia.Results
Even in expert hands, thick blood films were considerably less sensitive than might have been expected from the parasite numbers measured by quantitative PCR. In vitro work showed that thick films prepared from malaria cultures at known parasitaemia consistently underestimated parasite densities.Conclusion
It appears large numbers of parasites are lost during staining. This limits their sensitivity, and leads to erroneous estimates of parasite density. 相似文献45.
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48.
Restriction mapping and close relationship of the DNA of Streptomyces erythraeus phages 121 and SE-5 总被引:1,自引:0,他引:1
R Brzezinski E Surmacz M Kutner A Piekarowicz 《Journal of general microbiology》1986,132(10):2937-2943
The biological properties and genome structure of two actinophages, 121 and SE-5, infecting Streptomyces erythraeus were characterized. They had the same host range (limited to S. erythraeus) and similar DNA G + C contents (around 60 mol %). Restriction maps of their genomes also showed many similarities. The close relationship between the two phages was confirmed by DNA hybridization experiments: large parts of their genomes were homologous, except for a segment in the middle of the map, where no hybridization was detected. 相似文献
49.
Kaur P Qiang-Fu Fuhrmann A Ros R Kutner LO Schneeweis LA Navoa R Steger K Xie L Yonan C Abraham R Grace MJ Lindsay S 《Biophysical journal》2011,(1):243-250
Force spectroscopy and recognition imaging are important techniques for characterizing and mapping molecular interactions. In both cases, an antibody is pulled away from its target in times that are much less than the normal residence time of the antibody on its target. The distribution of pulling lengths in force spectroscopy shows the development of additional peaks at high loading rates, indicating that part of the antibody frequently unfolds. This propensity to unfold is reversible, indicating that exposure to high loading rates induces a structural transition to a metastable state. Weakened interactions of the antibody in this metastable state could account for reduced specificity in recognition imaging where the loading rates are always high. The much weaker interaction between the partially unfolded antibody and target, while still specific (as shown by control experiments), results in unbinding on millisecond timescales, giving rise to rapid switching noise in the recognition images. At the lower loading rates used in force spectroscopy, we still find discrepancies between the binding kinetics determined by force spectroscopy and those determined by surface plasmon resonance—possibly a consequence of the short tethers used in recognition imaging. Recognition imaging is nonetheless a powerful tool for interpreting complex atomic force microscopy images, so long as specificity is calibrated in situ, and not inferred from equilibrium binding kinetics. 相似文献
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