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81.
Kurt Preissler 《Hydrobiologia》1980,73(1-3):199-203
Avoidance of shore by pelagic rotifers is considered to be the result of an optical orientation. Field experiments show that the spatial light distribution in the shore region determines the preferred direction of migration. The behaviour of Eudiaptomus gracilis was tested in comparison to that of rotifers.This publication is dedicated to Pater Dr. Josef Donner on the occasion of his 70th birthday 相似文献
82.
Control of Plasmid R1 Replication: Kinetics of Replication in Shifts Between Different Copy Number Levels 总被引:2,自引:2,他引:0 下载免费PDF全文
Plasmid R1 replication was studied in shifts between two steady states of copy number. The copy number was varied in two ways. First, we utilized the fact that it decreases with increasing growth rate. To minimize the metabolic effects of changes in the growth rate, the downshifts were obtained by adding α-methylglucoside to cultures growing in glucose-minimal medium, and the upshifts were obtained by adding glucose to cultures growing in the presence of glucose plus α-methylglucoside. Second, we used a temperature-dependent copy mutant of plasmid R1 (pKN301). Plasmid pPK301 shows a threefold higher copy number at 40 than at 30°C. In both types of shift, plasmid replication immediately adjusted to the postshift differential rate. The copy number asymptotically adjusted to the new steady state. Hence, the system that controls plasmid R1 replication sets the frequency of replication without measuring the actual copy number. It has been suggested that plasmid R1 replication is under negative control by an R1-mediated repressor protein. Among the replication control models that involve negative control, the Pritchard inhibitor dilution model, the Sompayrac-Maaløe autorepressor model, and the plasmid λdv system all predict gene dose-independent copy number control. 相似文献
83.
A strain of Acremonium kiliense (Fungi Imperfecti) produced a water-soluble, dialyzable, heat-stable agent that rendered Cladophora glomerata (Chlorophyta) chlorotic and inhibited its growth. 相似文献
84.
The effect of human interferons on different types of lymphocyte-mediated killer assays was explored. Killing by T cells generated through mixed lymphocyte cultures as well as antibody-dependent lymphocyte-mediated cytotoxicity was not influenced by the addition of interferon. Enhancement of cytolysis produced by natural killer cells was observed when interferon was added during the assay, but enhancement could also be induced if the effector cells were pretreated with interferon for 2 hr prior to the lytic reaction. Killing of a cell line susceptible to natural killing was increased and a cell line which is normally relatively resistant to this type of killing became a susceptible target. 相似文献
85.
Kurt Mendgen 《Archives of microbiology》1979,122(2):129-135
A new species of anaerobic bacterium that degrades the even-numbered carbon fatty acids, butyrate, caproate and caprylate, to acetate and H2 and the odd-numbered carbon fatty acids, valerate and heptanoate, to acetate, propionate and H2 was obtained in coculture with either an H2-utilizing methanogen or H2-utilizing desulfovibrio. The organism could be grown only in syntrophic association with the H2-utilizer and no other energy sources or combination of electron donor and acceptors were utilized. It was a Gram-negative helical rod with 2 to 8 flagella, about 20 nm in diameter, inserted in a linear fashion about 130 nm or more apart along the concave side of the cell. It grew with a generation time of 84 h in co-culture with Methanospirillum hungatii and was present in numbers of at least 4.5×10-6 per g of anaerobic digestor sludge. 相似文献
86.
Encystment of Physarum polycephalum myxamoebae, grown under nearly identical physiological conditions as plasmodia is induced by transfer to a salts medium containing 0.5 M mannitol or mannose. After 24 h induction approximately 50% of amoebae had differentiated to cells which were identified to be young cysts by light and electron microscopy. Several other polyols, sugars, biogenic amines, and a starvation period from 24 h to one week caused no reproducible cyst formation. In contrast to the formation of dormant forms in the plasmodial stage of the life cycle, the induction of cysts and their germination to amoebae are not inhibited neither by actinomycin C nor by cycloheximide. In addition, the isoenzyme spectra of aminopeptidases and acid proteases remain nearly identical in growing and differentiating amoebae.Abbreviations SD semi-defined
BSS basal salts solution
The investigation is a part of the Ph. D. thesis of A. Haars, Göttingen, 1976 相似文献
87.
Aqueous samples containing labile substances are rapidly lyophilized after the frozen solutions are crushed in Nalgene plastic
bottles. Lyophilization time compares favorably with shell-frozen samples, and samples remain frozen at all times. 相似文献
88.
Kurt Schwarz 《Plant Systematics and Evolution》1979,131(3-4):193-209
89.
The 13C nmr chemical shifts of the common amino acid residues were measured in D2O solutions of the linear tetrapeptides H-Gly-Gly-X-L -Ala-OH. For Asp, Glu, Lys, Tyr and His, the titration shifts arising from the ionization of te amino acid side chains were also obtained. These data are compared with the corresponding 13C chemical shifts in the protected tetrapeptides CF3CO-Gly-Gly-X-L -Ala-OCH3, the linear pentapeptides H-Gly-Gly-X-Gly-Gly-OH, and the free amino acids. On this basism the selection of suitable “random coil” 13C chemical shifts for conformational studies of polypeptides chain is discussed. 相似文献
90.
Mlina Vaurs Julien Audry Kurt W Runge Vincent Gli Stphane Coulon 《Nucleic acids research》2022,50(20):11682
Telomere elongation is coupled with genome replication, raising the question of the repair of short telomeres in post-mitotic cells. We investigated the fate of a telomere-repeat capped end that mimics a single short telomere in quiescent fission yeast cells. We show that telomerase is able to elongate this single short telomere during quiescence despite the binding of Ku to the proto-telomere. While Taz1 and Rap1 repress telomerase in vegetative cells, both shelterin proteins are required for efficient telomere extension in quiescent cells, underscoring a distinct mode of telomerase control. We further show that Rad3ATR and Tel1ATM are redundantly required for telomere elongation in quiescence through the phosphorylation of Ccq1 and that Rif1 and its associated-PP1 phosphatases negatively regulate telomerase activity by opposing Ccq1 phosphorylation. The distinct mode of telomerase regulation in quiescent fission yeast cells may be relevant to that in human stem and progenitor cells. 相似文献