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961.
Competition between herbivorous insects often occurs as a trait mediated indirect effect mediated by inducible changes in plant quality rather than a direct effect mediated by plant biomass. While plant-mediated competition likely influences many herbivores, progress linking studies of plant-mediated competition in terrestrial phytophagous insects to longer-term consequences for herbivore communities has been elusive, and there is little relevant theory to guide this effort. We present simple models describing plant-mediated interactions between two herbivorous insects or other functionally equivalent organisms. These models consider general features of plant-mediated competition including specificity of elicitation by and effects on herbivores, positive and negative interactions among herbivores, competition independent of changes in plant biomass, and the existence of multiple relevant plant traits. Our analyses generate four important conclusions. First, herbivores competing strongly via only one plant quality phenotype exhibit a limited range of outcomes. These include coexistence and competitive exclusion of either herbivore, but do not include initial condition dependence. Second, when the outcome of competition is competitive exclusion, the herbivore that persists is the one that can do so under the highest inducible reductions in plant quality. Third, competition via more than one inducible phenotype can exhibit a wider range of outcomes including multiple equilibria and initial condition dependence. Finally, transient dynamics may not predict the eventual outcome of competition when changes in plant quality are slow relative to herbivore population growth, especially when herbivores compete through multiple phenotypes. We interpret our results in terms of competition outcomes reported in the literature, and suggest directions for the future empirical study of herbivore competition mediated by inducible changes in plant quality. 相似文献
962.
Shinichiro Shoji Nimo M. Abdi Ralf Bundschuh Kurt Fredrick 《Nucleic acids research》2009,37(12):4033-4042
Structural studies have revealed multiple contacts between the ribosomal P site and tRNA, but how these contacts contribute to P-tRNA binding remains unclear. In this study, the effects of ribosomal mutations on the dissociation rate (koff) of various tRNAs from the P site were measured. Mutation of the 30S P site destabilized tRNAs to various degrees, depending on the mutation and the species of tRNA. These data support the idea that ribosome-tRNA interactions are idiosyncratically tuned to ensure stable binding of all tRNA species. Unlike deacylated elongator tRNAs, N-acetyl-aminoacyl-tRNAs and tRNAfMet dissociated from the P site at a similar low rate, even in the presence of various P-site mutations. These data provide evidence for a stability threshold for P-tRNA binding and suggest that ribosome-tRNAfMet interactions are uniquely tuned for tight binding. The effects of 16S rRNA mutation G1338U were suppressed by 50S E-site mutation C2394A, suggesting that G1338 is particularly important for stabilizing tRNA in the P/E site. Finally, mutation C2394A or the presence of an N-acetyl-aminoacyl group slowed the association rate (kon) of tRNA dramatically, suggesting that deacylated tRNA binds the P site of the ribosome via the E site. 相似文献
963.
Marvin J. Miller Helen Zhu Yanping Xu Chunrui Wu Andrew J. Walz Anne Vergne John M. Roosenberg Garrett Moraski Albert A. Minnick Julia McKee-Dolence Jingdan Hu Kelley Fennell E. Kurt Dolence Li Dong Scott Franzblau Francois Malouin Ute Möllmann 《Biometals》2009,22(1):61-75
Pathogenic microbes rapidly develop resistance to antibiotics. To keep ahead in the “microbial war”, extensive interdisciplinary
research is needed. A primary cause of drug resistance is the overuse of antibiotics that can result in alteration of microbial
permeability, alteration of drug target binding sites, induction of enzymes that destroy antibiotics (ie., beta-lactamase)
and even induction of efflux mechanisms. A combination of chemical syntheses, microbiological and biochemical studies demonstrate
that the known critical dependence of iron assimilation by microbes for growth and virulence can be exploited for the development
of new approaches to antibiotic therapy. Iron recognition and active transport relies on the biosyntheses and use of microbe-selective
iron-chelating compounds called siderophores. Our studies, and those of others, demonstrate that siderophores and analogs
can be used for iron transport-mediated drug delivery (“Trojan Horse” antibiotics) and induction of iron limitation/starvation
(Development of new agents to block iron assimilation). Recent extensions of the use of siderophores for the development of
novel potent and selective anticancer agents are also described. 相似文献
964.
Puccinia horiana Henn. is a quarantine organism and one of the most important fungal pathogens of Chrysanthemum x morifolium cultivars grown for cut flower or potted plant production (florist's chrysanthemum) in several regions of the world. Highly specific primer pairs were identified for conventional, nested, and real-time PCR detection of P. horiana based on the specific and sensitive PCR amplification of selected regions in the internal transcribed spacers (ITS1 and ITS2) of the nuclear ribosomal DNA (rDNA). Using these different PCR versions, 10 pg, 10 fg, and 5 fg genomic DNA could be detected, respectively. When using cloned target DNA as template, the detection limits were 5000, 50, and 5 target copies, respectively. These detection limits were not affected by a background of chrysanthemum plant DNA. The DNA extraction method was optimized to maximize the recoverability of the pathogen from infected plant tissue. A CTAB extraction protocol or a selection of commercial DNA extraction methods allowed the use of 10 ng total (plant+pathogen) DNA without interference of PCR inhibitors. Due to the specificity of the primers, SYBR Green I technology enabled reliable real time PCR signal detection. However, an efficient TaqMan probe is available. The lowest proportion of infected plant material that could still be detected when mixed with healthy plant material was 0.001%. The real-time PCR assay could detect as few as eight pure P. horiana basidiospores, demonstrating the potential of the technique for aerial detection of the pathogen. The amount of P. horiana DNA in plant tissue was determined at various time points after basidiospore inoculation. Using the real-time PCR protocol, it was possible to detect the pathogen immediately after the inoculation period, even though the accumulation of pathogen DNA was most pronounced near the end of the latent period. The detection system proved to be accurate and sensitive and could help not only in pathogen diagnosis but also in pathogen monitoring and disease forecasting systems. 相似文献
965.
Biochemical characterization of a novel dual-function arabinofuranosidase/xylosidase isolated from a compost starter mixture 总被引:1,自引:0,他引:1
The gene encoding a glycoside hydrolase family 43 enzyme termed deAX was isolated and subcloned from a culture seeded with
a compost starter mixed bacterium population, expressed with a C-terminal His6-tag, and purified to apparent homogeneity. deAX was monomeric in solution and had a broad pH maximum between pH 5.5 and pH
7. A twofold greater k
cat/K
m for the p-nitrophenyl derivative of α-l-arabinofuranose versus that for the isomeric substrate β-d-xylopyranose was due to an appreciably lower K
m for the arabinofuranosyl substrate. Substrate inhibition was observed for both 4-methylumbelliferryl arabinofuranoside and
the xylopyranoside cogener. While no loss of activity was observed over 4 h at 40°C, the observed t
1/2 value rapidly decreased from 630 min at 49°C to 47 min at 53°C. The enzyme exhibited end-product inhibition, with a K
i for xylose of 145 mM, 18.5 mM for arabinose, and 750 mM for glucose. Regarding natural substrate specificity, deAX had arabinofuranosidase
activity on sugar beet arabinan, 1,5-α-l-arabinobiose, and 1,5-α-l-arabinotriose, and wheat and rye arabinoxylan, while xylosidase activity was detected for the substrates xylobiose, xylotriose,
xylotetraose, and arabinoxylan from beech and birch. Thus, deAX can be classified as a dual-function xylosidase/arabinofuranosidase
with respect to both artificial and natural substrate specificity. 相似文献
966.
Husson SJ Landuyt B Nys T Baggerman G Boonen K Clynen E Lindemans M Janssen T Schoofs L 《Peptides》2009,30(3):449-457
Neuropeptides are important signaling molecules that function in cell-cell communication as neurotransmitters or hormones to orchestrate a wide variety of physiological conditions and behaviors. These endogenous peptides can be monitored by high throughput peptidomics technologies from virtually any tissue or organism. The neuropeptide complement of the soil nematode Caenorhabditis elegans has been characterized by on-line two-dimensional liquid chromatography and quadrupole time-of-flight tandem mass spectrometry (2D-nanoLC Q-TOF MS/MS). Here, we use an alternative peptidomics approach combining liquid chromatography (LC) with matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry to map the peptide content of C. elegans and another Caenorhabditis species, Caenorhabditis briggsae. This study allows a better annotation of neuropeptide-encoding genes from the C. briggsae genome and provides a promising basis for further evolutionary comparisons. 相似文献
967.
Social interactions are among the most potent stressors. However, social allies may diminish stress, increase success in agonistic encounters and ease access to resources. We studied the role of social support as a major mechanism for individual stress management in families of free-ranging greylag geese (Anser anser). Greylag geese are long-term monogamous, live in a female-bonded social system, and fledged offspring stay with their parents until the next breeding season (‘primary families’). Should parents then fail to fledge young, subadults might rejoin them in summer after molt is completed (‘secondary families’). We have previously shown that primary greylag goose families reap benefits from active social support in agonistic encounters, and also excrete lower levels of immuno-reactive corticosterone metabolites (CORT, ‘passive social support’). Here we investigated how far active and passive social support continues in secondary goose families. Although we found that active support in agonistic encounters was almost absent in secondary families, subadult male geese won an increased number of agonistic encounters due to the mere presence of their secondary family. Particularly adult and subadult females benefited from passive social support through decreased CORT, whereas males did not. Decrease in the hormonal stress response during challenging situations, induced by social allies, may help the females' long-term energy management, thereby improving the odds for successful future reproduction. We discuss whether joining a secondary family may be an alternative tactic for young geese towards optimizing their start into a complex social life. 相似文献
968.
Macropodumines B and C (1, 2) are fused pentacyclic alkaloids previously isolated from the Chinese medicinal plant Daphniphyllum macropodum. Macropodumine B (1) possesses an amazing structural feature of a cyclopentadienyl carbanion, stabilized as a zwitterion by an internal iminium cation, which was confirmed by X-ray diffraction analysis. On the basis of the X-ray geometry, the absolute configuration of 1 was determined by employing the solid-state CD/TDDFT approach. The absolute configuration of 2 was instead determined by using TDDFT CD calculations on DFT-optimized geometries, in comparison with CD spectra recorded in solution. 相似文献
969.
Kathryn M. Langin Peter P. Marra Zoltán Németh Frank R. Moore T. Kurt Kyser Laurene M. Ratcliffe 《Journal of avian biology》2009,40(3):309-316
Each spring, millions of songbirds migrate across the Gulf of Mexico on their way to breeding sites in North America. Data from radar and migration monitoring stations have revealed broad patterns in the spatial and temporal course of trans-Gulf migration. Unfortunately, we have limited information on where these birds have previously spent the winter and where they are migrating to breed. Here we measure stable-hydrogen isotopes in feathers (δDf ) to infer the breeding latitude of five species of songbirds – hooded warblers Wilsonia citrina , American redstarts Setophaga ruticilla , black-and-white warblers Mniotilta varia , ovenbirds Seiurus aurocapilla , and northern waterthrushes S. noveboracensis – that were captured at a stopover site along the coast of southwestern Louisiana in spring 2004. Values of δDf across all species ranged from −163 to −35‰ (n=212), and within most species the range was consistent with the latitudinal extent of known breeding sites in central and eastern North America. Individuals that arrived first along the northern Gulf coast had δDf values indicative of southerly breeding sites in hooded warblers, American redstarts, black-and-white warblers, and ovenbirds, but no relationship was found between passage timing and δDf for northern waterthrushes. Our findings suggest that spring passage is often timed to coincide with the emergence of suitable conditions on breeding areas, with southern breeding birds migrating first. 相似文献
970.