全文获取类型
收费全文 | 140篇 |
免费 | 1篇 |
出版年
2018年 | 1篇 |
2015年 | 1篇 |
2014年 | 3篇 |
2013年 | 14篇 |
2012年 | 4篇 |
2011年 | 8篇 |
2010年 | 4篇 |
2009年 | 3篇 |
2008年 | 8篇 |
2007年 | 6篇 |
2006年 | 6篇 |
2005年 | 5篇 |
2004年 | 5篇 |
2003年 | 2篇 |
2002年 | 7篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 5篇 |
1998年 | 2篇 |
1997年 | 2篇 |
1996年 | 3篇 |
1995年 | 2篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1990年 | 1篇 |
1989年 | 4篇 |
1988年 | 6篇 |
1987年 | 6篇 |
1986年 | 4篇 |
1985年 | 3篇 |
1984年 | 4篇 |
1983年 | 2篇 |
1979年 | 4篇 |
1977年 | 2篇 |
1976年 | 5篇 |
1975年 | 1篇 |
1973年 | 1篇 |
1971年 | 1篇 |
排序方式: 共有141条查询结果,搜索用时 15 毫秒
71.
Wochna A Niemczyk E Kurono C Masaoka M Kedzior J Słomińska E Lipiński M Wakabayashi T 《Mitochondrion》2007,7(1-2):119-124
Apoptosis is induced not only during morphogenesis and embryogenesis but also under various pathological conditions, especially related to oxidative stress. Apoptotic cells are phagocytized by neighboring cells while necrotic cells cause local and general reactions sometimes lethal to our bodies. Data have been accumulated to demonstrate that the switch of the cell death mode from apoptosis to necrosis does occur. However, detailed mechanisms involved in the switch mechanism remain unsolved although decreases in the intracellular level of ATP and a burst in the cellular level of reactive oxygen species (ROS) have been proposed. Recently, we have shown that the population of apoptotic cells reaches maximum in human osteosarcoma 143B cells treated for 6h with menadione (MEN) while necrotic cells become predominant at 9h of the treatment. In the present study we have attempted to clarify the role of cellular ATP in the switch mechanism using rho(0) cells derived from human osteosarcoma rho+ cells. Results are summarized as follows: (1) Apoptotic and necrotic changes in rho(0) cells are much faster than rho+ cells after the treatment with MEN. (2) Cellular level of ATP in rho(0) cells remains essentially in the same level before and after the MEN-treatment while intracellular levels of superoxide continuously increase after the MEN-treatment. (3) rho+ cells treated with MEN in the presence of antimycin A plus oligomycin show similar changes to those of MEN-treated rho(0) cells. (4) MEN-induced increases in the cellular level of superoxide are distinctly suppressed by inhibitors of NADPH oxidase. These results suggest that the intracellular level of superoxide may be a key factor directly related to the switch mechanism from apoptosis to necrosis, and that decreases in cellular level of ATP accelerate both apoptotic and necrotic changes of the cells. 相似文献
72.
Shingo Nakada Hideo Takahashi Masayoshi Sakakura Masuo Kurono Ichio Shimada 《Biomolecular NMR assignments》2007,1(1):125-127
LolA is an essential periplasmic protein in Gram-negative bacteria and plays a role in transporting lipoproteins through periplasmic
space from the inner to the outer membrane. We established backbone resonance assignments of 2H/13C/15N labeled LolA from Escherichia coli. 相似文献
73.
74.
Abstract Using Cellophane-sheet techniques, changes in nucleotide pool size during conidia formation of Penicillium chrysogenum on agar plates were studied. On conidia-forming medium (nutritionally poor medium), the pool size of nucleoside triphosphates, especially GTP, decreased dramatically at mid-growth phase, concomitantly with the exhaustion of extracellular phosphate. Onset of conidia formation was observed just after the GTP pool decrease. 相似文献
75.
Niwayama S Kurono S Cho H Matsumoto H 《Bioorganic & medicinal chemistry letters》2006,16(23):6054-6057
D-labeled and -unlabeled N-beta-naphthyliodoacetamides have been synthesized for specific modification of the sulfhydryl groups of cysteine residues in proteins or peptides, and have been applied to quantitative analysis of several peptides. A combination of these reagents, coupled with mass spectrometry, is anticipated to serve as a useful tool for quantitative analysis of peptides and hence proteins. 相似文献
76.
77.
78.
Mitsunori Kono Takahiro Matsumoto Toru Kawamura Atsushi Nishimura Yoshihiro Kiyota Hideyuki Oki Junichi Miyazaki Shigeru Igaki Craig A. Behnke Masato Shimojo Masakuni Kori 《Bioorganic & medicinal chemistry》2013,21(1):28-41
A series of piperazine ureas was designed, synthesized, and evaluated for their potential as novel orally available fatty acid amide hydrolase (FAAH) inhibitors that are therapeutically effective against pain. We carried out an optimization study of the lead compound 3 to improve its DMPK profile as well as in vitro potency. We identified the thiazole compound 60j with potent inhibitory activity, high brain permeability, and good bioavailability. Compound 60j showed a potent and dose-dependent anti-nociceptive effect in the acetic acid-induced writhing test in mice. 相似文献
79.
Recently, we discovered a novel phenomenon, "cell-to-cell transformation" by which non-conjugative plasmids are transmitted horizontally in co-cultures of Escherichia coli F(-) strains. In this study, we aimed to identify the DNA element responsible for the high cell-to-cell transformability of pHSG299. By transplanting pHSG299 DNA fragments into pHSG399, a plasmid showing low transformability, we discovered that a specific 88 bp fragment of pHSG299 significantly promoted pHSG399 transformability. Although several short motif-like repetitive sequences (6-10 bp) were present in the 88 bp sequence, no known DNA motifs were recognized, suggesting that this 88 bp sequence (cell-to-cell transformation promoting sequence, CTPS; Accession number: AB634455) is a novel DNA element. 相似文献
80.
Nishio Y Kimura H Sawada N Sugaru E Horiguchi M Ono M Furuta Y Sakai M Masui Y Otani M Hashizuka T Honda Y Deguchi J Nakagawa T Nakahira H 《Bioorganic & medicinal chemistry》2011,19(18):5490-5499
We report on the identification of 2-({6-[(3R)-3-amino-3-methylpiperidine-1-yl]-1,3-dimethyl-2,4-dioxo-1,2,3,4-tetrahydro-5H-pyrrolo[3,2-d]pyrimidine-5-yl}methyl)-4-fluorobenzonitrile (DSR-12727) (7a) as a potent and orally active DPP-4 inhibitor without mechanism-based inactivation of CYP3A. Compound 7a showed good DPP-4 inhibitory activity (IC(50)=1.1 nM), excellent selectivity against related peptidases and other off-targets, good pharmacokinetic and pharmacodynamic profile, great in vivo efficacy in Zucker-fatty rat, and no safety concerns both in vitro and in vivo. 相似文献