全文获取类型
收费全文 | 42984篇 |
免费 | 16807篇 |
国内免费 | 1595篇 |
出版年
2024年 | 39篇 |
2023年 | 246篇 |
2022年 | 675篇 |
2021年 | 1278篇 |
2020年 | 2785篇 |
2019年 | 4397篇 |
2018年 | 4458篇 |
2017年 | 4556篇 |
2016年 | 4728篇 |
2015年 | 5008篇 |
2014年 | 4818篇 |
2013年 | 5400篇 |
2012年 | 3395篇 |
2011年 | 2858篇 |
2010年 | 3852篇 |
2009年 | 2571篇 |
2008年 | 1558篇 |
2007年 | 1022篇 |
2006年 | 948篇 |
2005年 | 939篇 |
2004年 | 755篇 |
2003年 | 741篇 |
2002年 | 672篇 |
2001年 | 580篇 |
2000年 | 477篇 |
1999年 | 426篇 |
1998年 | 172篇 |
1997年 | 178篇 |
1996年 | 172篇 |
1995年 | 136篇 |
1994年 | 144篇 |
1993年 | 103篇 |
1992年 | 148篇 |
1991年 | 125篇 |
1990年 | 121篇 |
1989年 | 101篇 |
1988年 | 91篇 |
1987年 | 80篇 |
1986年 | 80篇 |
1985年 | 68篇 |
1984年 | 65篇 |
1983年 | 52篇 |
1982年 | 49篇 |
1981年 | 28篇 |
1980年 | 24篇 |
1979年 | 35篇 |
1978年 | 30篇 |
1977年 | 28篇 |
1976年 | 29篇 |
1973年 | 25篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
91.
Xiaoqing Wu Xiaorui Xie Linjuan Su Na Lin Bin Liang Nan Guo Qingquan Chen Liangpu Xu Hailong Huang 《Journal of cellular and molecular medicine》2021,25(18):8929-8935
Pallister-Killian syndrome (PKS) is a rare sporadic genetic disorder usually caused by mosaicism of an extra isochromosome of 12p (i(12p)). This retrospective study analysed the prenatal ultrasound manifestations and molecular and cytogenetic results of five PKS foetuses. Samples of amniotic fluid and/or cord blood, skin biopsy and placenta were collected. Conventional karyotyping and single nucleotide polymorphism array (SNP array) were performed on all the amniotic fluid or cord blood samples. Copy number variants sequencing (CNV-seq) and fluorescence in situ hybridization (FISH) were also used for the validation for one foetus. All the five foetuses were from pregnancies with advanced parental age. Two foetuses involved structural abnormalities and one foetus had only soft markers, all of which included increased nuchal translucency. The rest two foetuses had normal ultrasounds in the second trimester, which has rarely been reported before. The karyotype revealed typical i(12p) in four cases and a small supernumerary marker chromosome consisting of 12p and 20p in the remaining one case. The proportion of cells with i(12p) ranged from 0 to 100% in cultural cells, while SNP array results suggested 2−4 copies of 12p. For one foetus, metaphase FISH showed normal results, but the interphase FISH suggested cell lines with two, three and four copies of 12p in the amniotic fluid. Advanced parental age may be an important risk factor for PKS, and there were no typical ultrasound manifestations related to PKS. A combination of karyotype analysis and molecular diagnosis is an effective method for the diagnosis of PKS. 相似文献
92.
T. RIGOT M. VERCAUTEREN DRUBBEL J.‐C. DELÉCOLLE M. GILBERT 《Medical and veterinary entomology》2013,27(1):29-38
The spatial epidemiology of Bluetongue virus (BTV) at the landscape level relates to the fine‐scale distribution and dispersal capacities of its vectors, midges belonging to the genus Culicoides Latreille (Diptera: Ceratopogonidae). Although many previous researches have carried out Culicoides sampling on farms, little is known of the fine‐scale distribution of Culicoides in the landscape immediately surrounding farms. The aim of this study was to gain a better understanding of Culicoides populations at increasing distances from typical dairy farms in north‐west Europe, through the use of eight Onderstepoort‐type black‐light traps positioned along linear transects departing from farms, going through pastures and entering woodlands. A total of 16 902 Culicoides were collected in autumn 2008 and spring 2009. The majority were females, of which more than 97% were recognized as potential vectors. In pastures, we found decreasing numbers of female Culicoides as a function of the distance to the farm. This pattern was modelled by leptokurtic models, with parameters depending on season and species. By contrast, the low number of male Culicoides caught were homogeneously distributed along the transects. When transects entered woodlands, we found a higher abundance of Culicoides than expected considering the distance of the sampling sites to the farm, although this varied according to species. 相似文献
93.
94.
95.
D A Hartman S R Kuo T R Broker L T Chow R D Wells 《The Journal of biological chemistry》1992,267(8):5488-5494
A conformational distortion in the DNA duplex at the regulatory region of human papillomavirus type-11 next to an intermolecular triplex, formed with a synthetic oligonucleotide, was investigated with several chemical probes. The sequence targeted for triplex formation borders on the binding sites for the regulatory proteins encoded by the viral E2 open reading frame. Dimethyl sulfate, diethyl pyrocarbonate, and OsO4 all react to a greater extent with nucleotides in the duplex that are immediately adjacent to the triplex as compared to other bases throughout the duplex. This hypermodification was observed on both the polypurine and polypyrimidine strands of the duplex DNA. Similar hyperreactivity of bases flanking a triplex also was seen when the contiguous target polypurine tract was effectively extended by mutating interrupting pyrimidines in the human papillomavirus type-11 sequence to purines. We propose that this hyperreactivity is due to a structural distortion caused by the junction between the triplex and the duplex tracts. 相似文献
96.
97.
98.
Distinct effects of climate warming on populations of silver fir (Abies alba) across Europe 下载免费PDF全文
99.
Integrated species delimitation and conservation implications of an endangered weevil Pachyrhynchus sonani (Coleoptera: Curculionidae) in Green and Orchid Islands of Taiwan 下载免费PDF全文
YEN‐TING CHEN HUI‐YUN TSENG MING‐LUEN JENG YONG‐CHAO SU WEN‐SAN HUANG CHUNG‐PING LIN 《Systematic Entomology》2017,42(4):796-813
Oceanic islands are productive habitats for generating new species and high endemism, which is primarily due to their geographical isolation, smaller population sizes and local adaptation. However, the short divergence times and subtle morphological or ecological divergence of insular organisms may obscure species identity, so the cryptic endemism on islands may be underestimated. The endangered weevil Pachyrhynchus sonani Kôno (Coleoptera: Curculionidae: Entiminae: Pachyrhynchini) is endemic to Green Island and Orchid Island of the Taiwan‐Luzon Archipelago and displays widespread variation in coloration and host range, thus raising questions regarding its species boundaries and degree of cryptic diversity. We tested the species boundaries of P. sonani using an integrated approach that combined morphological (body size and shape, genital shape, coloration and cuticular scale), genetic (four genes and restriction site‐associated DNA sequencing, RAD‐seq) and ecological (host range and distribution) diversity. The results indicated that all the morphological datasets for male P. sonani, except for the colour spectrum, reveal overlapping but statistically significant differences between islands. In contrast, the morphology of the female P. sonani showed minimum divergence between island populations. The populations of P. sonani on the two islands were significantly different in their host ranges, and the genetic clustering and phylogenies of P. sonani established two valid evolutionary species. Integrated species delimitation combining morphological, molecular and ecological characters supported two distinct species of P. sonani from Green Island and Orchid Island. The Green Island population was described as P. jitanasaius sp.n. Chen & Lin, and it is recommended that its threatened conservation status be recognized. Our findings suggest that the inter‐island speciation of endemic organisms inhabiting both islands may be more common than previously thought, and they highlight the possibility that the cryptic diversity of small oceanic islands may still be largely underestimated. 相似文献
100.