全文获取类型
收费全文 | 962篇 |
免费 | 44篇 |
国内免费 | 2篇 |
出版年
2022年 | 6篇 |
2021年 | 8篇 |
2020年 | 7篇 |
2019年 | 8篇 |
2018年 | 5篇 |
2017年 | 9篇 |
2016年 | 15篇 |
2015年 | 22篇 |
2014年 | 24篇 |
2013年 | 52篇 |
2012年 | 43篇 |
2011年 | 39篇 |
2010年 | 34篇 |
2009年 | 30篇 |
2008年 | 50篇 |
2007年 | 46篇 |
2006年 | 56篇 |
2005年 | 46篇 |
2004年 | 58篇 |
2003年 | 62篇 |
2002年 | 61篇 |
2001年 | 26篇 |
2000年 | 17篇 |
1999年 | 24篇 |
1998年 | 12篇 |
1997年 | 10篇 |
1996年 | 11篇 |
1995年 | 8篇 |
1994年 | 9篇 |
1993年 | 9篇 |
1992年 | 11篇 |
1991年 | 8篇 |
1990年 | 12篇 |
1989年 | 12篇 |
1988年 | 13篇 |
1987年 | 5篇 |
1986年 | 9篇 |
1985年 | 11篇 |
1984年 | 10篇 |
1983年 | 11篇 |
1982年 | 10篇 |
1981年 | 13篇 |
1980年 | 15篇 |
1979年 | 7篇 |
1978年 | 6篇 |
1977年 | 6篇 |
1976年 | 6篇 |
1975年 | 6篇 |
1972年 | 6篇 |
1970年 | 6篇 |
排序方式: 共有1008条查询结果,搜索用时 15 毫秒
31.
When thiamine concentration in subculture medium was rapidlylowered to nil, soybean cells in suspension became necroticand stopped growing entirely. When it was gradually lowered,cell growth was vigorous until the concentration was reducedto 7.8?103 mg/liter. The cells at this level of thiamineceased growing for a time, but prolonged culture in the samemedium resulted in the appearance of fresh white cells whichcould be easily distinguished from the old brown, necrotic cellsin the aggregates. These new cell lines could be subculturedwith further reduction in the thiamine supply, growing as largeraggregates of about 4 mm in diameter. New cell lines were similarly obtained by prolonged culturesin media containing a thiamine precursor; three lines appearedto be habituated to the pyrimidine moiety and one to the thiazolemoiety. The latter cell line could be subcultured without thiamineand its precursors for at least eight passages. These habituatedcells were characterized by the increase of the dry to freshweight ratio and by their growth in large aggregates.
1Present address: Section of Phytochemical Research, Eisai Co.,Ltd., Kawashima, Gifu 483, Japan. (Received December 15, 1978; ) 相似文献
32.
33.
Hans Holtschmidt Konrad Sandhoff Werner Fürst Hae Young Kwon Doris Schnabel Kunihiko Suzuki 《FEBS letters》1991,280(2):267-270
The organization of 14 exons covering 97% of the cDNA sequence of human cerebroside sulfate activator protein precursor has been determined from two overlapping EMBL-4 human genomic clones extending over 17kb. All exons and exon/intron splice junctions and five introns were sequenced. Exon 8 consists of only 9 bp and is involved in alternative splicing which generates three different mRNAs of cerebroside sulfate activator precursor. 相似文献
34.
Hidema Jun; Makino Amane; Kurita Yasuko; Mae Tadahiko; Ojima Kunihiko 《Plant & cell physiology》1992,33(8):1209-1214
Effects of irradiance on changes in the amounts of chlorophyll(Chl) and light-harvesting chlorophyll a/b protein of PS II(LHCII) were examined in senescing leaves of rice (Oryza sativaL.). Results of treatments at two irradiances (100% and 20%natural sunlight) were examined after the full expansion ofthe 13th leaf throughout the course of senescence. With 20%sunlight, the Chl content decreased only a little during leafsenescence, while with 100% sunlight it decreased appreciably.Similarly, the amount of LHCII protein during treatment with20% sunlight remained almost constant. However, the ratio ofChl a/b during the shade treatment decreased significantly andthe rate of decrease was greater than during the full-sunlighttreatment. The ratio of Chl a/b for Chl a and b bound to LHCIIwas about 1.2, irrespective of leaf age or irradiance treatment.When the amounts of Chl bound to LHCII were calculated fromthe total leaf content of Chl and the ratio of Chl a/b, assuminga ratio of Chl a/b bound to LHCII of 1.2, they were well correlatedwith the amounts of LHCII protein. Changes in the amounts of LHCII synthesized during the two irradiancetreatments were examined using an 15 tracer. Incorporation of15N into LHCII declined dramatically during both treatmentsfrom full expansion through senescence, suggesting that therewas little synthesis of LHCII protein during that time. In addition,the amount of LHCII synthesized during senescence was lowerduring the shade treatment than during the 100% sunlight treatment.These results indicate that the absence of an apparent changein levels of LHCII with shade treatment during senescence wascaused by the very low rate of turnover of LHCII protein. (Received June 17, 1992; Accepted September 28, 1992) 相似文献
35.
Tadami Nagao Mitsumoto Komatsuda Kunihiko Yamauchi Shigeru Arimori 《Journal of cellular physiology》1981,108(2):155-161
In a liquid culture of human bone marrow, the development of fibroblast colonies takes place on days 6 to 9. Twenty percent fetal calf serum is used as the stimulus for fibroblast colony growth. Human bone marrow cells are plated as 2 × 105 cells in the culture. Normal human bone marrow yields 47 ± 4 fibroblasts colonies per 2 × 105 cells plated. Bone marrow fibroblast cultures using agar or methylcellulose restrict colony formation. Marked colony suppression was observed in acute leukemia, and a discrete colony number was observed in hypoplastic anemia. This fibroblast culture method should be applied to a larger number of patients to determine whether it has a pathognomonic value and clinical significance. 相似文献
36.
Kunihiko Kimura Yutaka Takahashi Masayoshi Konishi Sotaro Iwamoto 《Primates; journal of primatology》1981,22(1):119-130
On the basis of 44 hindlimbs of 14 male and 14 female crab-eating monkeys (Macaca fascicularis), the morphology of the adductor muscles of the thigh was described and some functional indices were calculated. The results
obtained from this study agreed generally with those of otherMacaca species reported by various authors. For the classification and nomenclature of the adductors, the criteria proposed byUhlmann (1967, 1968) was well adapted to the crab-eating monkey. The adductors comprise the m. gracilis, m. pectineus, m. adductor
longus, pars longa and pars brevis of m. adductor brevis, pars lata and pars minima of m. adductor magnus and m. obturatorius
externus. In males, the adductors are generally inserted further down the femur, and the insertions of pars brevis of the
m. adductor brevis and pars minima of the m. adductor magnus have longer attachments to the femur than in females. The arrangement
of each adductor muscle and of each fasciculus of a thigh muscle may invoke a principle of organization. 相似文献
37.
A supernumerary muscle bundle “M. tubero-femoralis
Fick” of the biceps femoris was found in a female crab-eating monkey in both hindlimbs. 相似文献
38.
During the course of partially synchronized cell divisions in cultured tobacco (Xanthi) cells the amount of endogenous cytokinins in the butanol-soluble fraction increased 5 to 10 times in 3 hours and paralleled the increase in frequency of mitosis. Among three cytokinins detected in tobacco cells, the activity corresponding to the RF of authentic zeatin in thin layer chromatography changed in parallel with the mitotic index. 相似文献
39.
40.
Identification of Cytokinins of Root Nodules of the Garden Pea, Pisum sativum L 总被引:4,自引:3,他引:1
下载免费PDF全文
![点击此处可从《Plant physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Five cytokinin activities which induced soybean callus proliferation were detected in ethanol extracts of root nodules of the garden pea (Pisum sativum L., cv. Little Marvel). The most active factors among them were identified as zeatin and its riboside on the basis of their mobility on thin layer chromatography in three solvent systems. Smaller activities of zeatin ribotide, isopentenyladenine and its riboside were also detected. Cytokinin activity gradually decreased with the cultivation period, but no qualitative change in the active compounds was found. 相似文献