全文获取类型
收费全文 | 119篇 |
免费 | 1篇 |
出版年
2023年 | 1篇 |
2021年 | 3篇 |
2019年 | 2篇 |
2018年 | 2篇 |
2017年 | 4篇 |
2015年 | 1篇 |
2014年 | 5篇 |
2012年 | 7篇 |
2011年 | 9篇 |
2010年 | 4篇 |
2009年 | 5篇 |
2008年 | 4篇 |
2007年 | 1篇 |
2006年 | 1篇 |
2005年 | 2篇 |
2004年 | 7篇 |
2003年 | 5篇 |
2002年 | 2篇 |
2001年 | 2篇 |
2000年 | 1篇 |
1999年 | 2篇 |
1998年 | 6篇 |
1997年 | 1篇 |
1992年 | 2篇 |
1990年 | 4篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 4篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1981年 | 1篇 |
1980年 | 2篇 |
1979年 | 4篇 |
1978年 | 4篇 |
1977年 | 2篇 |
1976年 | 2篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1971年 | 2篇 |
1970年 | 2篇 |
1967年 | 1篇 |
1965年 | 1篇 |
排序方式: 共有120条查询结果,搜索用时 15 毫秒
41.
G. E. Bronnikov T. P. Kulagina A. V. Aripovsky 《Biochemistry (Moscow) Supplemental Series A: Membrane and Cell Biology》2009,3(4):453-458
The effect of a potent antioxidant, flavonoid dihydroquercetin on the activity of three mitochondrial enzymes in mouse skeletal
muscles has been investigated. An ability of this substance to restore the activity of mitochondrial enzymes in old animals
was demonstrated. The activities of citrate synthase, NADHcoenzymeQ1-oxidoreductase (complex 1) and cytochromc-oxidase (complex 4) were assessed using spectro-photometric analysis in a quadriceps muscle homogenate. It was shown that
the citrate synthase activity decreased moderately and the activities of complexes 1 and 4 in skeletal muscles dropped significantly
in old mice. Supplementation of drink water with dihydroquercetin for a few weeks led to an increase of citrate synthase and
complex 1 activity (P < 0.1) in muscles of old animals. Activity of complex 4 returned to the level found in the tissue of young mice. Maximal
activity of citrate synthase and complex 1 was found in muscles of young mice. Sensitivity of NADH-coenzymeQ1-oxidoreductase
to a specific inhibitor rotenone differed in all three groups of mice. Young and old mice exhibited about 95% and 84% of the
total sensitivity, respectively, while in old mice receiving dihydroquercetin the sensitivity of complex 1 to the inhibitor
increased up to 98%. The biochemical alterations entailed an increase in animals’ mobility as well as an improvement of fur
and skin condition. Fatty acid composition of homogenate in muscle tissue of all three groups was also investigated. A reliable
decline of the amount of linoleic acid and an increase in stearic and docosanoic acid contents as well as an increase of total
amount of fatty acids in muscles of old mice were found. Statistically significant changes in fatty acid composition in muscles
of old mice in the control group and in old mice receiving antioxidant were not observed. 相似文献
42.
43.
Synthesis of lipids was studied in isolated nuclei from rat thymus and liver cells. On incubation of the isolated nuclei with [2-14C]acetate and [1-14C]glycerol, the label was intensively incorporated into phospholipids and with a significantly lower intensity into fatty acids and cholesterol. Only trace amounts of radioactivity were detected in the lipids of chromatin prepared from isolated thymus nuclei after their incubation, and this suggested that lipids were mainly synthesized on the nuclear membrane. On the preincubation of thymus tissue homogenate with [2-14C]acetate and the subsequent isolation of the nuclei and chromatin, the radioactivity of chromatin lipids was comparable to the radioactivity of nuclear lipids. The findings suggested that in the isolated nuclei the newly synthesized lipids were not transported into chromatin from the nuclear membrane. The specific radioactivities of individual phospholipids and fatty acids were different in the isolated nuclei and in nuclei obtained from preincubated homogenate. Mechanisms of lipid synthesis in isolated nuclei and causes of the different radioactivities of lipids in the isolated nuclei and in the nuclei obtained from the preincubated homogenate are discussed. 相似文献
44.
In computer models of spinal motoneurons with reconstructed dendritic arborizations, we performed a comparative study of the
features of the dendritic morphology and those of the output discharge patterns generated in response to stepwise or triangular
ramp depolarizing currents applied to the soma; the modeled motoneurons were taken from wild-type (WT) and mutant Cu/Zn superoxide
dismutase 1 (SOD1) animals. Six dendrites of the SOD1 motoneuron formed a more complex arborization than a 10-dendrite branching
of the WT cell. Although both arborizations had similar maximum path distances of about 600 μm, a maximum dendritic complexity
of the SOD1 cell was found at path distances of about 300 μm from the soma (about 100 μm farther than that of the WT cell).
A greater number of dendritic paths terminating at longer path distances from the soma formed also different morphometrical
asymmetry patterns of SOD1 arborizations, as compared with those of WT ones. The model neurons were capable of reproducing
several types of experimentally recorded discharge patterns described in the literature. Main differences between the two
cells were observed in the hysteretic frequency-current relations characterizing the discharges caused by the ramp current.
Compared to the WT motoneuron, the SOD1 cell (i) had a wider dynamic range of the current intensities at which the discharge
was initiated and terminated, (ii) this range was shifted to greater current intensities, and (iii) the maximum discharge
frequency was lower. The nature of the found structure-related differences still has to be explored and interpreted considering
that SOD1 mutants mimic some cases of amyotrophic lateral sclerosis. 相似文献
45.
46.
T P Kulagina 《Radiobiologiia》1990,30(6):745-748
The phospholipid content of the homogenate, nuclei and chromatin of rat thymus was being studied during three months after fractionated gamma-irradiation (2 Gy X 3 at a week interval). The number of phospholipids in the total fraction of phosphatidyl choline + phosphatidyl serine and phosphatidyl ethanolamine in the nuclei and chromatin of rat thymus was shown to decrease 60 min following the last exposure. In a month the phospholipid content in the nuclei and chromatin increased up to the control level keeping it throughout the entire period of observation. 相似文献
47.
Shkoporov AN Khokhlova EV Kulagina EV Smeianov VV Kafarskaia LI Efimov BA 《Bioscience, biotechnology, and biochemistry》2008,72(3):742-748
Bifidobacteria and Bacteroides-like bacteria are strictly anaerobic nonpathogenic members of human intestinal microflora. Here we describe an analysis of the species and subspecies composition of these bacterial populations in healthy children using a combination of culture and molecular methods at two different time points. It was found that B. bifidum and B. longum are the most common dominant taxons in infants aged between 8 and 16 months. The majority of the infants carried several dominant Bifidobacterium strains belonging to different species. Examination of the dominant bifidoflora in some of these children after a 5-year period showed major shifts in both species and strain composition, but the dominant strains remained unchanged in two children. The majority of dominant Bacteroides-like isolates belonged to species B. vulgatus and B. uniformis, but members of genera Alistipes and Barnesiella were common too. In addition, a novel approach to species identification of Bacteroidales order bacteria using amplified ribosomal DNA restriction analysis (ARDRA) is described. 相似文献
48.
N N Kulagina N D Ivanovskaia I A Taranova A Ia Kul'berg 《Biulleten' eksperimental'no? biologii i meditsiny》1986,101(2):185-187
Biosynthesis of C1q complement component by resident peritoneal macrophages from (CBA X C57BL/6)F1 mice has been studied in in vitro experiments. Using anti-mouse C1q antibodies immobilized on CNBr Sepharose it has been demonstrated that 14C glycine incorporates both into intracellular C1q and C1q secreted into the medium. The maximum radioactivity of intracellular C1q was observed 48 h after cultivation, with it dropping drastically between hours 72-96. Kinetics of radiolabelled C1q was similar, but 24 hours delayed. Cell viability during 96 h of cultivation remained unchanged. These data can be considered as the indication of feedback regulation of C1q biosynthesis at the cellular level. 相似文献
49.
T P Kulagina I K Kolomi?tseva E A Obol'nikova G I Samokhvalov A M Kuzin 《Biulleten' eksperimental'no? biologii i meditsiny》1992,113(3):253-254
The influence of continuous gamma irradiation on the lipids of nuclei and chromatin of rat liver at a dose-rate of 0,129 Gy/day for 155 days (a total dose of 20 Gy) and by feeding of ubiquinone-9 has been studied. The amount of phosphatidylcholine with phosphatidylserine and phosphatidyl-ethanolamine in liver nuclei of irradiated rats was found to increase. Ubiquinone-9 had a normalizing effect. A decrease of cardiolipin was observed in the liver chromatin of irradiated rats. The amount of free fatty acids had a tendency to decrease in homogenate, nuclei and liver chromatin of irradiated rats. Ubiquinone was found to increase the amount of free fatty acids up to the control level. The amount of cholesterol in nuclei was increased after irradiation and that in chromatin tended to rise. Ubiquinone-9 significantly decreased the amount of cholesterol in nuclei and chromatin of irradiated rats. 相似文献
50.