全文获取类型
收费全文 | 566篇 |
免费 | 83篇 |
专业分类
649篇 |
出版年
2023年 | 3篇 |
2022年 | 8篇 |
2021年 | 9篇 |
2020年 | 4篇 |
2019年 | 4篇 |
2018年 | 3篇 |
2017年 | 6篇 |
2016年 | 13篇 |
2015年 | 22篇 |
2014年 | 17篇 |
2013年 | 28篇 |
2012年 | 34篇 |
2011年 | 40篇 |
2010年 | 24篇 |
2009年 | 29篇 |
2008年 | 35篇 |
2007年 | 58篇 |
2006年 | 34篇 |
2005年 | 37篇 |
2004年 | 32篇 |
2003年 | 23篇 |
2002年 | 17篇 |
2001年 | 17篇 |
2000年 | 26篇 |
1999年 | 31篇 |
1998年 | 6篇 |
1997年 | 7篇 |
1996年 | 10篇 |
1995年 | 5篇 |
1994年 | 8篇 |
1993年 | 6篇 |
1992年 | 6篇 |
1991年 | 5篇 |
1990年 | 11篇 |
1989年 | 6篇 |
1988年 | 3篇 |
1987年 | 5篇 |
1986年 | 4篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1982年 | 1篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1977年 | 2篇 |
1975年 | 2篇 |
1973年 | 2篇 |
1970年 | 1篇 |
排序方式: 共有649条查询结果,搜索用时 0 毫秒
641.
We have studied the role of Tyr-69 of porcine pancreatic phospholipase A2 in catalysis and substrate binding, using site-directed mutagenesis. A mutant was constructed containing Phe at position 69. Kinetic characterization revealed that the Phe-69 mutant has retained enzymatic activity on monomeric and micellar substrates, and that the mutation has only minor effects on kcat and Km. This shows that Tyr-69 plays no role in the true catalytic events during substrate hydrolysis. In contrast, the mutation has a profound influence on the stereospecificity of the enzyme. Whereas the wild-type phospholipase A2 is only able to catalyse the degradation of sn-3 phospholipids, the Phe-69 mutant hydrolyses both the sn-3 isomers and, at a low (1-2%) rate, the sn-1 isomers. Despite the fact that the stereospecificity of the mutant phospholipase has been altered, Phe-69 phospholipase still requires Ca2+ ions as a cofactor and also retains its specificity for the sn-2 ester bond. Our data suggest that in porcine pancreatic phospholipase A2 the hydroxyl group of Tyr-69 serves to fix and orient the phosphate group of phospholipid monomers by hydrogen bonding. Because no such interaction can occur between the Phe-69 side-chain and the phosphate moiety of the substrate monomer, the mutant enzyme loses part of its stereospecificity but not its positional specificity. 相似文献
642.
Delivery of liposome-associated drugs to liver cells 总被引:1,自引:0,他引:1
643.
Kuipers H.; Costill D. L.; Porter D. A.; Fink W. J.; Morse W. M. 《Journal of applied physiology》1986,61(3):859-863
This investigation studied the effect of an oral glucose feeding on glycogen sparing during exercise in non-glycogen-depleted and glycogen-depleted endurance-trained rats. The non-glycogen-depleted rats received via a stomach tube 2 ml of a 20% glucose solution labeled with [U-14C]glucose just prior to exercise (1 h at 25 m/min). Another group of rats ran for 40 min at higher intensity to deplete glycogen stores, after which they received the same glucose feeding and continued running for 1 h at 25 m/min. The initial 40-min run depleted glycogen in heart, skeletal muscle, and liver. In the non-glycogen-depleted rats the glucose feeding spared glycogen in the liver, primarily from the oxidation of blood-borne glucose in muscle. In the glycogen-depleted rats, muscle glycogen was repleted after the feeding, but sources other than the administered glucose also contributed to glycogen synthesis. The results suggest that glycogen depletion rather than the glucose feeding per se stimulates glycogen resynthesis in muscle during exercise in endurance-trained rats. 相似文献
644.
645.
Nathan T Kuipers Charity L Sauder Jason R Carter Chester A Ray 《Journal of applied physiology》2008,104(4):1129-1136
The purpose of this study was to determine neurovascular responses to mental stress (MS) in the supine and upright postures. MS was elicited in 23 subjects (26 +/- 1 yr) by 5 min of mental arithmetic. In study 1 (n = 9), Doppler ultrasound was used to measure mean blood flow velocity in the renal (RBFV) and superior mesenteric arteries (SMBFV), and venous occlusion plethysmography was used to measure forearm blood flow (FBF). In study 2 (n = 14), leg blood flow (LBF; n = 9) was measured by Doppler ultrasound, and muscle sympathetic nerve activity (MSNA; n = 5) was measured by microneurography. At rest, upright posture increased heart rate and MSNA and decreased LBF, FBF, RBFV, and SMBFV and their respective conductances. MS elicited similar increases in mean arterial blood pressure ( approximately 12 mmHg) and heart rate ( approximately 17 beats/min), regardless of posture. MS in both postures elicited a decrease in RBFV, SMBFV, and their conductances and an increase in LBF, FBF, and their conductances. Changes in blood flow were blunted in the upright posture in all vascular beds examined, but the pattern of the vascular response was the same as the supine posture. MS did not change MSNA in either posture (change: approximately 1 +/- 3 and approximately 3 +/- 3 bursts/min, respectively). In conclusion, the augmented sympathetic activity of the upright posture does not alter heart rate, mean arterial blood pressure, or MSNA responses to MS. MS elicits divergent vascular responses in the visceral and peripheral vasculature. These results indicate that, although the upright posture attenuates vascular responses to MS, the pattern of neurovascular responses does not differ between postures. 相似文献
646.
Nicholas JG Webster Lui-Guojing Evans Matt Caples Laura Erker Shern L Chew 《BMC molecular biology》2004,5(1):7-15
Background
Incorporation of exon 11 of the insulin receptor gene is both developmentally and hormonally-regulated. Previously, we have shown the presence of enhancer and silencer elements that modulate the incorporation of the small 36-nucleotide exon. In this study, we investigated the role of inherent splice site strength in the alternative splicing decision and whether recognition of the splice sites is the major determinant of exon incorporation. 相似文献647.
648.
649.
James B. Kramer Diane H. Boschelli David T. Connor Catherine R. Kostlan Daniel L. Flynn Richard D. Dyer Dirk A. Bornemeier John A. Kennedy Clifford D. Wright Paul J. Kuipers 《Bioorganic & medicinal chemistry letters》1992,2(12):1655-1660
Replacement of the carboxylic acid function of inodmethacin with reversed hydroxamic acids converted this selective cyclooxygenase (CO) inhibitor into dual inhibitors of CO and 5-lipoxygenase (5-LO). 相似文献