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141.
Arthur H. L. From Dwight S. Fullerton Khalil Ahmed 《Molecular and cellular biochemistry》1990,94(2):157-165
The structure-activity relationships of the genin moieties of digitalis glycosides are commonly elucidated by determining the inhibitory potency of a variety of genins toward the plasma membrane Na+, K+-ATPase; qualitatively these relationships appear to be fairly independent of the specific Na+, K+-ATPase preparation utilized for the analysis. To determine whether this is the case with regard to the sugar moieties of glycosides, the inhibitory effects of 12 monoglycosides of digitoxigenin toward four Na+, K+-ATPase preparations of different origin were measured. It was found that while recognition of the major structural determinants of sugar activity appeared to be independent of enzyme source, recognition of the minor structural determinants of activity showed some source dependence. It was also observed that the intrinsic sensitivity to sugar potentiation may be source dependent and unrelated to intrinsic sensitivity to inhibition by digitoxigenin. These observations are compatible with a model of the Na+, K+-ATPase sugar binding site(s) in which intrinsic sensitivity to sugar attachment as well as recognition characteristics (for sugar structural features) both determine the extent to which a sugar moiety may contribute to the activity of monoglycosides. Further, in these studies one of the Na+, K+-ATPase preparations employed was obtained from rat brain, a tissue known to contain a mixture of ouabain sensitive and insensitive isoforms. We have observed that the rigorous purification techniques employed appear to have selectively removed from or denatured the less ouabain sensitive al isoform found in this enzyme preparation. 相似文献
142.
143.
P1 nuclease defines a subpopulation of active SV40 chromatin--a new nuclease hypersensitivity assay. 总被引:1,自引:0,他引:1
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Under exhaustive digestion conditions P1 nuclease was found to cleave a subpopulation of intracellular SV40 chromatin only once. The major P1 cleavage site in SV40 DNA was mapped at the origin of DNA replication, and the two minor sites at the SV40 enhancers. The P1-sensitive SV40 chromatin subpopulation was found to have higher superhelical density than the bulk of the intracellular SV40 chromatin. Furthermore, pulse labeled SV40 DNA which had higher superhelical density than that of the steady state viral DNA (S.S.Chen and M.T.Hsu, J.Virol 51:14-19, 1984) was also found to be preferentially cleaved by P1 nuclease. These results are consistent with a supercoil-dependent alteration of chromatin conformation near the regulatory region of the viral genome that can be recognized by P1 nuclease. Since P1 nuclease cleaves the subpopulation of SV40 chromatin only once without further degradation, this nuclease can be used as a general tool to define viral or cellular chromatin fraction with altered chromatin conformation and to map nuclease hypersensitive sites. Preliminary studies indicate that P1 makes limited double stranded cleavages in cellular chromatin to generate large DNA fragments. 相似文献
144.
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146.
Arthur Yuwiler 《Neurochemical research》1990,15(1):95-100
Isatin (Tribulin) produced a dose-dependent inhibition of both MAO A and MAO B in broken cell preparations from rat brain and pineal. However, isatin administered in vivo (80–160 mg/kg) to the intact animal significantly increased brain, but not pineal, serotonin and did not affect 5HIAA or other indoles in either brain or pineal. Further, in vivo administration did not produce detectable MAO inhibition in either tissue. In pineal organ culture, addition of isatin up to 1mM had no influence on the concentrations of pineal indoles or the activities of monoamine oxidase or serotonin N-acetyltransferase. However, the diazepam augmentation of beta adrenergic induction of serotonin N-acetyltransferase activity was blocked by isatin. The results of these studies call into question the proposed role of isatin as an endogenous monoamine oxidase inhibitor but support a possible role as a benzodiazepine receptor blocker. 相似文献
147.
传入C纤维的兴奋在中电针“足三里”激活中缝大核中的作用 总被引:6,自引:0,他引:6
The purpose of the present work is to study whether the analgesia of "Zusanli" EA was mainly produced by its noxious effect. The antidromic C waves on N. peroneus communis innervating the area of "Zusanli" point were recorded. When "Zusanli" point was stimulated by trains of stimuli, the amplitude of the antidromic C wave was obviously decreased due to collision with the orthodromic stimulation. It was suggested that EA of "Zusanli" could excite some C fibers. It was observed that when the stimulation intensity reached the threshold of C fiber, the NRM neurons were obviously activated, and when it reached or exceeded the intensity for producing the maximal C wave, the NRM neurons were highly activated. Therefore, EA analgesia is probably produced mainly by its noxious stimulus component, especially carried by C fibers, via a negative feedback mechanism in modulating pain. 相似文献
148.
149.
Arthur C. Brown Michael H. Smolensky Gilbert E. D'Alonzo Daniel P. Redman 《Chronobiology international》1990,7(2):125-133
-Twenty-three diurnally active (0705-2333), healthy persons between 22 and 54 yrs of age and without history of sleep abnormality were monitored continuously for 120 consecutive hr (five days) by wrist actigraphy. Circadian rhythms of high amplitude were detected by cosinor analysis for each participant and for the groups of 10 males and 13 females with the average span of heightened activity timed between ∼1330 and 1605. The circadian peak-trough difference in wrist movement was marked, equalling aproximately 75% of the 24-hr mean level. In 19 of 23 participants, the 24-hr mean of wrist activity varied between 140-180 movements/min, with four persons exhibiting lesser means of 110-140 movements/min. With respect to the daytime span of activity, the mean wrist movement of individual participants ranged from 155-265 movements/min, with the majority (20/23) varying between 185-245 movements/min. During nocturnal sleep the mean wrist activity level was quite low, varying between individuals from 5 to 25 movements/min for 21 of 23 persons. Wrist actigraphy proved to be well-accepted and was a most reliable means of monitoring aspects of body movement during activity and sleep in ambulatory persons adhering to usual life habits and pursuits. 相似文献
150.
DNA from Guthrie spots for diagnosis of DMD by multiplex PCR 总被引:2,自引:0,他引:2
E R McCabe Y Huang M Descartes Y H Zhang R G Fenwick 《Biochemical medicine and metabolic biology》1990,44(3):294-295