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661.
Vanessa Kruse Carina Hamann Sebastian Monecke Lukas Cyganek Leslie Elsner Daniela Hübscher Lutz Walter Katrin Streckfuss-B?meke Kaomei Guan Ralf Dressel 《PloS one》2015,10(5)
Human induced pluripotent stem cells (hiPSCs) could be used to generate autologous cells for therapeutic purposes, which are expected to be tolerated by the recipient. However, iPSC-derived grafts are at risk of giving rise to teratomas in the host, if residuals of tumorigenic cells are not rejected by the recipient. We have analyzed the susceptibility of hiPSC lines to allogeneic and autologous natural killer (NK) cells. IL-2-activated, in contrast to resting NK cells killed hiPSC lines efficiently (P=1.69x10-39). Notably, the specific lysis of the individual hiPSC lines by IL-2-activated NK cells was significantly different (P=1.72x10-6) and ranged between 46 % and 64 % in 51Cr-release assays when compared to K562 cells. The hiPSC lines were killed by both allogeneic and autologous NK cells although autologous NK cells were less efficient (P=8.63x10-6). Killing was partly dependent on the activating NK receptor DNAM-1 (P=8.22x10-7). The DNAM-1 ligands CD112 and CD155 as well as the NKG2D ligands MICA and MICB were expressed on the hiPSC lines. Low amounts of human leukocyte antigen (HLA) class I proteins, which serve as ligands for inhibitory and activating NK receptors were also detected. Thus, the susceptibility to NK cell killing appears to constitute a common feature of hiPSCs. Therefore, NK cells might reduce the risk of teratoma formation even after autologous transplantations of pluripotent stem cell-derived grafts that contain traces of pluripotent cells. 相似文献
662.
Treatment of recurrent glioma with intracavitary alloreactive cytotoxic T lymphocytes and interleukin-2 总被引:4,自引:0,他引:4
C. A. Kruse Linda Cepeda Betty Owens Stephen D. Johnson John Stears Kevin O. Lillehei 《Cancer immunology, immunotherapy : CII》1997,45(2):77-87
For a single-dose toxicity assessment, five patients with recurrent malignant glioma (ages 29–46 years) were treated with
intracavitary alloreactive cytotoxic T lymphocytes (CTL) and interleukin-2 (IL-2). The trial tested the hypothesis that alloreactive
CTL, sensitized to the major histocompatibility complex (MHC) proteins of the patient, offer selective, targeted killing of
glioma cells that express MHC. Patient lymphocytes, which also express MHC, were irradiated and placed into CellMax artificial
capillary systems with lymphocytes from MHC-disparate donors and CTL developed over a 2- to 3-week period with a low concentration
of IL-2. The CTL largely expressed CD3 and CD11a/CD8 markers and lysed targets displaying patient MHC. CTL were implanted
into the tumor bed at surgery and a catheter was used for subsequent infusions. Patients received one to five treatment cycles
every other month; one cycle generally consisted of two or three CTL infusates administered within a 1- to 2-week period.
Different unrelated donors were used for each cycle. Treatment was well tolerated; transient toxicity at grades 1–3 was recorded
by NCI Common Toxicity Scale criteria. Two glioblastoma patients have died; one from tumor recurrence locally and the other
from recurrence at a site distant from the treatment. Two of the five patients completed five cycles; one anaplastic oligodendroglioma
patient shows no evidence of tumor 30 months from the start of immune therapy and an anaplastic astrocytoma patient shows
stable disease 28 months after initiation of therapy. One anaplastic oligodendroglioma patient, who dropped the protocol during
her second treatment cycle, has no evidence of tumor 28 months after recurrence.
Received: 21 May 1997 / Accepted: 17 July 1997 相似文献
663.
Neil A. Johnson Fengli Liu Phillip D. Weeks Hilary P. Kruse Mette Laursen Charles J. Costa 《Archives of biochemistry and biophysics》2009,481(2):157-29662
Recombinant Ca2+-ATPase from tomato (i.e. LCA1 for Lycopersicon esculentum [Since the identification and naming of LCA1, the scientific name for the tomato has been changed to Solanum lycopersicum.] Ca-ATPase) was heterologously expressed in yeast for structure-function characterization. We investigate the differences between plant and animal Ca pumps utilizing comparisons between chicken and rabbit SERCA-type pumps with Arabidopsis (ECA1) and tomato plant (LCA1) Ca2+-ATPases. Enzyme function was confirmed by the ability of each Ca2+-ATPase to rescue K616 growth on EGTA-containing agar and directly via in vitro ATP hydrolysis. We found LCA1 to be ∼300-fold less sensitive to thapsigargin than animal SERCAs, whereas ECA1 was thapsigargin-resistant. LCA1 showed typical pharmacological sensitivities to cyclopiazonic acid, vanadate, and eosin, consistent with it being a PIIA-type Ca2+-ATPase. Possible amino acid changes responsible for the reduced plant thapsigargin-sensitivity are discussed. We found that LCA1 also complemented K616 yeast growth in the presence of Mn2+, consistent with moving Mn2+ into the secretory pathway and functionally compensating for the lack of secretory pathway Ca-ATPases (SPCAs) in plants. 相似文献
664.
665.
666.
The present paper gives a report on changes of enzyme reactions, of the glycogen content, and the nucleolus picture in lymphocytes which are based on cytochemical investigations of blood smears taken from 110 children with different diseases. In 20 new-born babies the cytochemical responses of lymphocytes after triple immunization with Di-Pe-Te immunization matter were observed. The findings reveal significant changes to be found predominantly in the activity of alpha-naphthylacetate esterase, PAS-reaction and the nucleolus picture of lymphocytes in immunological reactions. No hints for specific immunological functions of lymphocytes could be detected. The changes may refer to B-lymphocytes and to T-lymphocytes. 相似文献
667.
Human diploid cells (CF-1) derived from newborn foreskin tissue were maintained in a non-mitotic state for as long as 177 days by reducing the serum concentration of the incubation medium to 0.5%. The cells could be returned to the proliferative state by subcultivation with normal growth medium containing 10% serum. Cells treated in such a manner reached passage levels equivalent to controls that had been continuously cultured on growth medium, but they took a proportionately longer calendar time to achieve the equivalent passage levels. Also, by using 3H-thymidine incorporation, cells held in the non-mitotic conditions showed a longer ‘predictable life span’ than control cultures. During 21-day maintenance periods there was a 10–20% cell loss and ca 30% loss of protein per cell. The finite life span of these human diploid cells was clearly related to the number of cumulative population doublings rather than to the total calendar time in vitro. 相似文献
668.
Eamonn J. Dickson Jill B. Jensen Oscar Vivas Martin Kruse Alexis E. Traynor-Kaplan Bertil Hille 《The Journal of cell biology》2016,213(1):33-48
Endoplasmic reticulum–plasma membrane (ER–PM) contact sites play an integral role in cellular processes such as excitation–contraction coupling and store-operated calcium entry (SOCE). Another ER–PM assembly is one tethered by the extended synaptotagmins (E-Syt). We have discovered that at steady state, E-Syt2 positions the ER and Sac1, an integral ER membrane lipid phosphatase, in discrete ER–PM junctions. Here, Sac1 participates in phosphoinositide homeostasis by limiting PM phosphatidylinositol 4-phosphate (PI(4)P), the precursor of PI(4,5)P2. Activation of G protein–coupled receptors that deplete PM PI(4,5)P2 disrupts E-Syt2–mediated ER–PM junctions, reducing Sac1’s access to the PM and permitting PM PI(4)P and PI(4,5)P2 to recover. Conversely, depletion of ER luminal calcium and subsequent activation of SOCE increases the amount of Sac1 in contact with the PM, depleting PM PI(4)P. Thus, the dynamic presence of Sac1 at ER–PM contact sites allows it to act as a cellular sensor and controller of PM phosphoinositides, thereby influencing many PM processes. 相似文献
669.
670.
Svenja Kruse 《Polar Biology》2009,32(9):1377-1387
In the Lazarev Sea, Southern Ocean, I studied the population structure and reproduction of Eukrohnia bathypelagica and E. bathyantarctica during winter and summer. A lack of unimodality in their population structures indicated at least two generations building
one population and a life cycle longer than 1 year. During both seasons all maturity stages were present; therefore, continuous
reproduction is very probable. Extensive breeding seasons with several releases of eggs by one generation are assumed, as
adults with empty marsupial sacs continue to build new ova. E. bathypelagica carried between 86 and 128 eggs in both marsupial sacs. E. bathyantarctica had only between 8 and 13 eggs. Although self-fertilization seemed at least to be possible in E. bathyantarctica, cross-fertilization appears to be more common in both species, as most individuals developed testes and ovaries consecutively. 相似文献