Mapping the amino acid properties of constituent nucleoporins onto the yeast nuclear pore complex

Visualization of molecular structures aids in the understanding of structural and functional roles of biological macromolecules. Macromolecular transport between the cell nucleus and cytoplasm is facilitated by the nuclear pore complex (NPC). The ring structure of the NPC is large and contains several distinct proteins (nucleoporins) which function as a selective gate for the passage of certain molecules into and out of the nucleus. In this note we demonstrate the utility of a python code that allows direct mapping of the physiochemical properties of the constituent nucleoporins on the scaffold of the yeast NPC׳s cytoplasmic view. We expect this tool to be useful for researchers to visualize the NPC based on their physiochemical properties and how it alters when specific mutations are introduced in one or more of the nucleoporins. The code developed using Python is available freely from the authors.     

Improvement of plasma energy confinement in tokamak under radiative cooling of the edge plasma

Improvement of plasma energy confinement in the T-10 tokamak by injection of impurity gases was studied experimentally. Injection of Ne and He in the ohmic and ECR heating regimes allows one to separate the dependences of energy confinement on the plasma density and on the edge plasma cooling rate. It is shown that the well-known dependence of the energy confinement time on the plasma density is, in fact, the dependence on the radiative loss power. This phenomenon can be explained by plasma self-organization. The experiments are described by a thermodynamic model for self-organized plasma in which the transport coefficient depends on the difference between the actual and self-consistent pressure profiles. The reduction in the heat flux at the plasma edge due to radiative cooling leads to a decrease in the transport coefficient in this region and, accordingly, improves energy confinement. Results of approximate model calculations for experiments with Ne injection are presented.     

Charge-exchange recombination spectroscopy of the plasma ion temperature at the T-10 tokamak

Charge-exchange recombination spectroscopy (CXRS) based on a diagnostic neutral beam has been developed at the T-10 tokamak. The diagnostics allows one to measure the ion temperature profile in the cross section of the plasma column. In T-10 experiments, the measurement technique was adjusted and the elements of the CXRS diagnostics for ITER were tested. The used spectroscopic equipment makes it possible to reliably determine the ion temperature from the Doppler broadening of impurity lines (helium, carbon), as well as of the spectral lines of the working gas. The profiles of the plasma ion temperature in deuterium and helium discharges were measured at different plasma currents and densities, including with the use of active Doppler measurements of lines of different elements. The validity and reliability of ion temperature measurements performed by means of the developed CXRS diagnostics are analyzed.     

Biological and physico-chemical properties of Yersinia pseudotuberculosis bacterial culture having the fra-operon Yersinia pestis

The biological and physico-chemical properties of cultures of two isogenous recombinant variants of Yersinia pseudotuberculosis were studied. The cell genomes of the cultures are distinguished from one another only by the presence or by the absence of the fra-operon, which is a determined attribute of the plague microbe capsule-forming process. The expression of the attribute is amplified by rising the microbial biomass cultivation temperature and stimulates the decrease in the viability of the bacteria and adaptation potential in vitro. In the warm-blooded owner organism the microbes of the capsule-forming recombinant variant are characterized by the greater residual pathogenicity and immunogenic ability to the experimental plague of the laboratory animals as compared to the reference-variant cells. These specific features could be explained by more expressed colonizing ability of the capsule-forming microbes provided by owner cells' stability to the phagocyte process.     

The treatment of isolated genera

Problems presented by genera, or small groups of genera, which have been given family rank are reviewed, and the genera are divided into a number of categories according to the closeness of their affinity to other genera or families. Satellite genera that stand in close relation to families should be united with them. Binary families, that have been divided into two (or more) related families, should be re–united. Families connected by linking genera, should, logically, be united but practical considerations usually prevent this. Clusters of diverse but more or less distantly related genera present unusual problems, being treated either as several, often monogeneric families or as a loosely structured family. Truly isolated genera must be given family and often ordinal rank.     

Investigation of the H-mode during ECRH in the T-10 tokamak

An improved confinement regime with an external transport barrier (H-mode) is obtained during electron-cyclotron resonance heating of a plasma in the T-10 tokamak. A characteristic feature of this regime is a spontaneous density growth accompanied by a drop in the intensity of D_α line and an increase in β_p by a factor of ～1.6. The threshold power for the L-H transition is close to that predicted by the ITER scaling. The best characteristics of the H-mode are achieved with decreasing q _L to 2.2. It is shown that the external transport barrier arises for electrons, whereas the heat transport barrier insignificantly contributes to improved confinement.