全文获取类型
收费全文 | 110篇 |
免费 | 8篇 |
国内免费 | 4篇 |
专业分类
122篇 |
出版年
2022年 | 2篇 |
2018年 | 6篇 |
2017年 | 3篇 |
2016年 | 7篇 |
2015年 | 11篇 |
2014年 | 14篇 |
2013年 | 6篇 |
2012年 | 5篇 |
2011年 | 6篇 |
2010年 | 7篇 |
2009年 | 6篇 |
2008年 | 5篇 |
2007年 | 2篇 |
2006年 | 3篇 |
2005年 | 5篇 |
2004年 | 5篇 |
2003年 | 4篇 |
2002年 | 1篇 |
2001年 | 3篇 |
2000年 | 2篇 |
1999年 | 1篇 |
1998年 | 5篇 |
1995年 | 1篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1990年 | 1篇 |
1988年 | 1篇 |
1985年 | 1篇 |
1983年 | 4篇 |
1978年 | 2篇 |
1973年 | 1篇 |
排序方式: 共有122条查询结果,搜索用时 125 毫秒
71.
Albert Eduardo Silva Martins Norma Lucena-Silva Renan Gomes Garcia Stefan Welkovic Aureliana Barboza Maria Luiza Bezerra Menezes Magda Maruza Terezinha Tenório Ricardo AA Ximenes 《Memórias do Instituto Oswaldo Cruz》2014,109(6):738-747
Human immunodeficiency virus (HIV)-positive patients have a greater prevalence ofcoinfection with human papillomavirus (HPV) is of high oncogenic risk. Indeed, thepresence of the virus favours intraepithelial squamous cell lesion progression andmay induce cancer. The aim of this study was to evaluate the prevalence of HPVinfection, distribution of HPV types and risk factors among HIV-positive patients.Cervical samples from 450 HIV-positive patients were analysed with regard to oncoticcytology, colposcopy and HPV presence and type by means of polymerase chain reactionand sequencing. The results were analysed by comparing demographic data and datarelating to HPV and HIV infection. The prevalence of HPV was 47.5%. Among theHPV-positive samples, 59% included viral types of high oncogenic risk. Multivariateanalysis showed an association between HPV infection and the presence of cytologicalalterations (p = 0.003), age greater than or equal to 35 years (p = 0.002), number ofpartners greater than three (p = 0.002), CD4+ lymphocyte count <200/mm3 (p = 0.041) and alcohol abuse (p = 0.004). Although high-riskHPV was present in the majority of the lesions studied, the low frequency of HPV 16(3.3%), low occurrence of cervical lesions and preserved immunological state in mostof the HIV-positive patients were factors that may explain the low occurrence ofprecancerous cervical lesions in this population. 相似文献
72.
Qingdong Guan Peyman Ezzati Victor Spicer Oleg Krokhin Donna Wall John A. Wilkins 《Clinical proteomics》2017,14(1):26
Background
Mesenchymal stem/stromal cells (MSC) display a range of immunoregulatory properties which can be enhanced by the exposure to cytokines such interferon γ (IFN-γ). However the compositional changes associated with the ‘licensing’ of these cells have not been clearly defined. The present study was undertaken to provide a detailed comparative proteomic analysis of the compositional changes that occur in human bone marrow derived MSC following 20 h treatment with IFN-γ.Methods
2D LC MSMS analysis of control and IFN-γ treated cells from 5 different healthy donors provided confident identification of more than 8400 proteins.Results
In total 210 proteins were shown to be significantly altered in their expression levels (≥|2SD|) following IFN-γ treatment. The changes for several of these proteins were confirmed by flow cytometry. STRING analysis determined that approximately 30% of the altered proteins physically interacted in described interferon mediated processes. Comparison of the list of proteins that were identified as changed in the proteomic analysis with data for the same proteins in the Interferome DB indicated that ~35% of these proteins have not been reported to be IFN-γ responsive in a range of cell types.Conclusions
This data provides an in depth analysis of the proteome of basal and IFN-γ treated human mesenchymal stem cells and it identifies a number of novel proteins that may contribute to the immunoregulatory capacity if IFN-γ licensed cells.73.
74.
Uwe Thissen Suzan Wopereis Sjoerd AA van den Berg Ivana Bobeldijk Robert Kleemann Teake Kooistra Ko Willems van Dijk Ben van Ommen Age K Smilde 《BMC bioinformatics》2009,10(1):52
Background
In the fields of life sciences, so-called designed studies are used for studying complex biological systems. The data derived from these studies comply with a study design aimed at generating relevant information while diminishing unwanted variation (noise). Knowledge about the study design can be used to decompose the total data into data blocks that are associated with specific effects. Subsequent statistical analysis can be improved by this decomposition if these are applied on selected combinations of effects. 相似文献75.
Background
Monetary incentives are an effective way of increasing response rates to surveys, though they are generally less effective in physicians, and are more effective when the incentive is paid up-front rather than when made conditional on completion. 相似文献76.
Geert Zegels Geert AA Van Raemdonck Wiebren AA Tjalma Xaveer WM Van Ostade 《Proteome science》2010,8(1):63
Cervicovaginal fluid has an important function in the homeostasis and immunity of the lower female genital tract. Analysis
of the cervicovaginal fluid proteome may therefore yield important information about the pathogenesis of numerous gynecological
pathologies. Additionally, cervicovaginal fluid has great potential as a source of biomarkers for these conditions. 相似文献
77.
Thymol and Carvacrol Prevent Cisplatin‐Induced Nephrotoxicity by Abrogation of Oxidative Stress,Inflammation, and Apoptosis in Rats 下载免费PDF全文
El‐Sayed EM Abd‐Allah AR Mansour AM EL‐Arabey AA 《Journal of biochemical and molecular toxicology》2015,29(4):165-172
The aim of the present study is to assess the possible protective effects of thymol and carvacrol against cisplatin (CP)‐induced nephrotoxicity. A single dose of CP {6 mg/kg, intraperitoneally (i.p.)} injected to male rats revealed significant increases in serum urea, creatinine, and tumor necrosis factor alpha levels. It also increased kidney contents of malondialdehyde and caspase‐3 activity with significant reduction in serum albumin, kidney content of reduced glutathione as well as catalase, and superoxide dismutase activity as compared to that of the control group. In contrast, administration of thymol {20 mg/kg, orally (p.o.)} and/or carvacrol (15 mg/kg, p.o.) for 14 days before CP injection and for 7 days after CP administration restored the kidney function and examined oxidative stress parameters. In conclusion, thymol was more effective nephroprotective than carvacrol. Moreover, a combination of thymol and carvacrol had a synergistic nephroprotective effect that might be attributed to antioxidant, anti‐inflammatory, and antiapoptotic activities. 相似文献
78.
McQueen P Spicer V Rydzak T Sparling R Levin D Wilkins JA Krokhin O 《Proteomics》2012,12(8):1160-1169
We have developed a real-time graphic-processor-unit-based search engine capable of high-quality peptide identifications in <500 μs per spectrum. The steps of peptide/protein identification, in-silico prediction of all possible tryptic peptides from these proteins, and the prediction of their expected retention times and m/z values take less than 5 s per cycle over ~3000 MS/MS spectra. This lays the foundation for information-dependent acquisition with exclusion lists generated on-the-fly, as the instrument continues to acquire data. While a complete evaluation of the dynamic exclusion system requires the participation from instrument vendors, we conducted a series of model experiments using a whole cell tryptic digestion of the bacterium Clostridium thermocellum. We ran a series of five iterative LC-MS/MS runs, adding a new exclusion list at each of four chromatographic "tripping points" - the elution times of the four standard peptides spiked into the sample. Retention times of these standard peptides were also used for real-time "chromatographic calibration." The dynamic exclusion approach gave a ≈ 5% increase in confident protein identification (for typical 2 h LC-MS/MS run), and reduced the average number of identified peptides per protein from 4.7 to 2.9. Its application to a two-times shorter gradient gave a ≈ 17% increase in proteins identified. Further improvements are possible for instruments with better mass accuracy, by employing a more accurate retention prediction algorithm and by developing better understanding of the possible chemical modifications and fragmentations produced during electrospray ionization. 相似文献
79.
80.
Integrated chorda tympani nerve responses to NaCl were studied in two mouse
strains, an NaCl-preferring NZB/B1NJ and an NaCl-avoiding CBA/J. The NaCl
responses of both strains had similar magnitude and were suppressed by
amiloride to a similar extent. This suggests that peripheral gustatory
responsiveness to NaCl is not the only mechanism underlying mouse strain
variation in NaCl acceptance.
相似文献