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91.
92.
Wang X Zheng L Zeng Z Zhou G Chien J Qian C Vasmatzis G Shridhar V Chen L Liu W 《Biochemical and biophysical research communications》2006,347(1):22-30
Ccd1, a DIX domain containing Zebrafish protein involved in neural patterning, is a positive regulator of the Wnt signaling pathway. DIXDC1, the human homolog of Ccd1, has two predominant isoforms. The short form (s-DIXDC1) has a similar amino acid sequence compared with Ccd1, while the long form (l-DIXDC1) contains an extra N-terminal sequence containing a calponin-homology (CH) domain, suggesting additional interaction with actin that we have performed detailed analysis in this report. We show that mRNA expression of both DIXDC1 isoforms can be detected in various adult tissues by Northern blot analysis and is most abundant in cardiac and skeletal muscles. Both endogenous and ectopically expressed l-DIXDC1, but not s-DIXDC1, in cultured mammalian cells is localized to actin stress fibers at the filament ends in focal adhesion plaques. More importantly, l-DIXDC1 can directly bind to filamentous actin both in vitro and in vivo and the binding is mediated via a novel actin-binding domain (ABD) from amino acid 127 to 300. Thus, our data provide the first evidence that l-DIXDC1 may act as a novel branching component in the Wnt signaling pathway targeting both beta-catenin-TCF complex for gene expression and cytoskeleton for regulating dynamics of actin filaments. 相似文献
93.
Lance W. Noll Pragathi B. Shridhar Diana M. Dewsbury Xiaorong Shi Natalia Cernicchiaro David G. Renter T. G. Nagaraja 《PloS one》2015,10(8)
Culture-based methods to detect the six major non-O157 (O26, O45, O103, O111, O121 and O145) Shiga toxin-producing E. coli (STEC) are not well established. Our objectives of this study were to develop a culture-based method to detect the six non-O157 serogroups in cattle feces and compare the detection with a PCR method. Fecal samples (n = 576) were collected in a feedlot from 24 pens during a 12-week period and enriched in E. coli broth at 40° C for 6 h. Enriched samples were subjected to immunomagnetic separation, spread-plated onto a selective chromogenic medium, and initially pooled colonies, and subsequently, single colonies were tested by a multiplex PCR targeting six serogroups and four virulence genes, stx1, stx2, eae, and ehxA (culture method). Fecal suspensions, before and after enrichment, were also tested by a multiplex PCR targeting six serogroups and four virulence genes (PCR method). There was no difference in the proportions of fecal samples that tested positive (74.3 vs. 77.4%) for one or more of the six serogroups by either culture or the PCR method. However, each method detected one or more of the six serogroups in samples that were negative by the other method. Both culture method and PCR indicated that O26, O45, and O103 were the dominant serogroups. Higher proportions (P < 0.05) of fecal samples were positive for O26 (44.4 vs. 22.7%) and O121 (22.9 vs. 2.3%) serogroups by PCR than by the culture method. None of the fecal samples contained more than four serogroups. Only a small proportion of the six serogroups (23/640; 3.6%) isolated carried Shiga toxin genes. The culture method and the PCR method detected all six serogroups in samples negative by the other method, highlighting the importance of subjecting fecal samples to both methods for accurate detection of the six non-O157 STEC in cattle feces. 相似文献
94.
Role of peripheral and central noradrenaline and 5-hydroxytryptamine in the carrageenin-induced pedal oedema in rats was studied
using agents which influence catecholamine synthesis and receptor activity of noradrenaline and 5-hydroxytryptamine. Reserpine,
guanethidine, α-methyl-p-tyrosine, diethyldithiocarbamate, 6-hydroxydopamine, phenoxybenzamine, phentolamine, chlorpromazine and yohimbine markedly
inhibited carrageenin-induced pedal oedema. However, 6-hydroxydopamine given intracerebroventricularly, 5,6-dihydroxytryptamine,p-chlorophenylalanine, lower dose of yohimbine, pro pranolol, haloperidol, cyproheptadine and mepyramine did not alter the
carrageenin-induced oedema, whereas, cyproheptadine and mepyramine given simultaneously, markedly inhibited carrageenin-induced
oedema. Our studies indicate that the process of oedema formation in rats by carrageenin involves both the peripheral noradrenaline
and 5-hydroxytryptamine
Communication No. 58 from IDPL Research Centre, Hyderabad. 相似文献
95.
96.
A Baldini N Archidiacono R Carbone A Bolino V Shridhar O J Miller D A Miller D C Ward M Rocchi 《Cytogenetics and cell genetics》1992,59(1):12-16
We have isolated and characterized a human genomic DNA clone (PZ20, locus D20Z2) that identifies, under high-stringency hybridization conditions, an alphoid DNA subset specific for chromosome 20. The specificity was determined using fluorescence in situ hybridization. Sequence analysis confirmed our previously reported data on the great similarity between the chromosome 20 and chromosome 2 alphoid subsets. Comparative mapping of pZ20 on chimpanzee and gorilla chromosomes, also performed under high-stringency conditions, indicates that the alphoid subset has ancestral sequences on chimpanzee chromosome 11 and gorilla chromosome 19. However, no hybridization was observed to chromosomes 21 in the great apes, the homolog of human chromosome 20. 相似文献