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71.
Kristin J. Bondo Craig K. R. Willis Jackie D. Metheny R. Julia Kilgour Erin H. Gillam Matina C. Kalcounis-Rueppell R. M. Brigham 《The Journal of wildlife management》2019,83(8):1753-1761
Understanding the ephemerality of trees used as roosts by wildlife, and the number of roost trees needed to sustain their populations, is important for forest management and wildlife conservation. Several studies indicate that roosts are limiting to bats, but few studies have monitored longevity of roost trees used by bats over several years. From 2004–2007 in Cypress Hills Interprovincial Park, Saskatchewan, Canada, several big brown bats (Eptesicus fuscus) from a maternity group roosted in cavities in trembling aspen (Populus tremuloides) trees approximately 7 km southeast away from their original known roosting area (RA1). Using a long-term data set of the roost trees used by bats in this area from 2000–2007, we evaluated whether the movement of bats to the new roosting area (RA4) corresponded with annual and cumulative losses of roost trees. We also determined whether longevity of the roosts from the time we discovered bats first using them differed between the 2 roosting areas based on Kaplan-Meier estimates. Bats began using RA4 in addition to RA1 in 2004, when the cumulative loss of roost trees in RA1 over 3 consecutive years reached 18%. Most bats exclusively roosted in RA4 in 2007, when the cumulative loss of roost trees over 6 consecutive years had reached 46% in RA1. Annual survival for roost trees, from when we first discovered bats using them, was generally lower in RA1 than in RA4. Our results suggest that the movement of bats to the new roosting area corresponded with high losses of roost trees in RA1. This provides additional evidence that to maintain high densities of suitable roost trees for bats in northern temperature forests over several decades, management plans need to recruit live and dead trees in multiple age classes and stages of decay that will be suitable for the formation of new cavities. © 2019 The Wildlife Society. 相似文献
72.
Aldi T. Kraja Chunyu Liu Jessica L. Fetterman Mariaelisa Graff Christian Theil Have Charles Gu Lisa R. Yanek Mary F. Feitosa Dan E. Arking Daniel I. Chasman Kristin Young Symen Ligthart W. David Hill Stefan Weiss Jian’an Luan Franco Giulianini Ruifang Li-Gao Fernando P. Hartwig Kari E. North 《American journal of human genetics》2019,104(1):112-138
73.
Christopher W. Schmidt Ashley Remy Rebecca Van Sessen John Willman Kristin Krueger Rachel Scott Patrick Mahoney Jeremy Beach Jaqueline McKinley Ruggero D'Anastasio Laura Chiu Michele Buzon J. Rocco De Gregory Susan Sheridan Jacqueline Eng James Watson Haagen Klaus Pedro Da-Gloria Jeremy Wilson Abigail Stone Paul Sereno Jessica Droke Rose Perash Christopher Stojanowski Nicholas Herrmann 《American journal of physical anthropology》2019,169(2):207-226
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Johannes R. Schmidt Sarah Vogel Stephanie Moeller Stefan Kalkhof Kristin Schubert Martin von Bergen Ute Hempel 《Journal of cellular biochemistry》2019,120(5):8706-8722
The development of novel bioactive biomaterials is urgently needed to meet the needs of an aging population. Both sulfated hyaluronic acid and dexamethasone are candidates for the functionalization of bone grafts, as they have been shown to enhance the differentiation of osteoblasts from bone marrow stromal cells in vitro and in vivo. However, the underlying mechanisms are not fully understood. Furthermore, studies combining different approaches to assess synergistic potentials are rare. In this study, we aim to gain insights into the mode of action of both sulfated hyaluronic acid and dexamethasone by a comprehensive analysis of the cellular fraction, released matrix vesicles, and the extracellular matrix, combining classical biochemical assays with mass spectrometry–based proteomics, supported by novel bioinformatical computations. We found elevated differentiation levels for both treatments, which were further enhanced by a combination of sulfated hyaluronic acid and dexamethasone. Single treatments revealed specific effects on osteogenic differentiation. Dexamethasone activates signalling pathways involved in the differentiation of osteoblasts, for example, CXC-motif chemokine receptor type 4 and mitogen-activated protein kinases. The effects of sulfated hyaluronic acid were predominantly linked to an alteration in the composition of the extracellular matrix, affecting the synthesis, secretion, and/or activity of fibrillary (fibronectin and thrombospondin-2) and nonfibrillary (transglutaminase-2, periostin, and lysyloxidase) extracellular matrix components, including proteases and their inhibitors (matrix metalloproteinase-2, tissue inhibitor of metalloproteinase-3). The effects were treatment specific, and less additive or contrary effects were found. Thus, we anticipate that the synergistic action of the treatment-specific effects is the key driver in elevated osteogenesis. 相似文献
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Anne M. Bronikowski Richard P. Meisel Peggy R. Biga James
R. Walters Judith E. Mank Erica Larschan Gerald S. Wilkinson Nicole Valenzuela Ashley Mae Conard Joo Pedro de Magalhes Jingyue
Duan Amy E. Elias Tony Gamble Rita
M. Graze Kristin E. Gribble Jill A. Kreiling Nicole C. Riddle 《Aging cell》2022,21(2)
Sex differences in aging occur in many animal species, and they include sex differences in lifespan, in the onset and progression of age‐associated decline, and in physiological and molecular markers of aging. Sex differences in aging vary greatly across the animal kingdom. For example, there are species with longer‐lived females, species where males live longer, and species lacking sex differences in lifespan. The underlying causes of sex differences in aging remain mostly unknown. Currently, we do not understand the molecular drivers of sex differences in aging, or whether they are related to the accepted hallmarks or pillars of aging or linked to other well‐characterized processes. In particular, understanding the role of sex‐determination mechanisms and sex differences in aging is relatively understudied. Here, we take a comparative, interdisciplinary approach to explore various hypotheses about how sex differences in aging arise. We discuss genomic, morphological, and environmental differences between the sexes and how these relate to sex differences in aging. Finally, we present some suggestions for future research in this area and provide recommendations for promising experimental designs. 相似文献
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Dhaval P. Bhatt C. Allie Mills Kristin A. Anderson Brbara J. Henriques Tnia G. Lucas Sara Francisco Juan Liu Olga R. Ilkayeva Alexander E. Adams Shreyas R. Kulkarni Donald S. Backos Michael B. Major Paul A. Grimsrud Cludio M. Gomes Matthew D. Hirschey 《The Journal of biological chemistry》2022,298(4)
A wide range of protein acyl modifications has been identified on enzymes across various metabolic processes; however, the impact of these modifications remains poorly understood. Protein glutarylation is a recently identified modification that can be nonenzymatically driven by glutaryl-CoA. In mammalian systems, this unique metabolite is only produced in the lysine and tryptophan oxidative pathways. To better understand the biology of protein glutarylation, we studied the relationship between enzymes within the lysine/tryptophan catabolic pathways, protein glutarylation, and regulation by the deglutarylating enzyme sirtuin 5 (SIRT5). Here, we identify glutarylation on the lysine oxidation pathway enzyme glutaryl-CoA dehydrogenase (GCDH) and show increased GCDH glutarylation when glutaryl-CoA production is stimulated by lysine catabolism. Our data reveal that glutarylation of GCDH impacts its function, ultimately decreasing lysine oxidation. We also demonstrate the ability of SIRT5 to deglutarylate GCDH, restoring its enzymatic activity. Finally, metabolomic and bioinformatic analyses indicate an expanded role for SIRT5 in regulating amino acid metabolism. Together, these data support a feedback loop model within the lysine/tryptophan oxidation pathway in which glutaryl-CoA is produced, in turn inhibiting GCDH function via glutaryl modification of GCDH lysine residues and can be relieved by SIRT5 deacylation activity. 相似文献