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861.
Odiere MR Opisa S Odhiambo G Jura WG Ayisi JM Karanja DM Mwinzi PN 《Parasitology》2011,138(12):1569-1577
This cross-sectional study determined the prevalence and distribution of schistosome and soil-transmitted helminth (STH) infections among 1,308 children aged 10-18 years in 34 primary schools in 8 informal urban settlements in Kisumu City, western Kenya. Stool samples were collected and examined for eggs of Schistosoma mansoni and STH (Hookworms, Ascaris lumbricoides and Trichuris trichiura) using the Kato-Katz technique. Haematuria was used as a proxy indicator of urinary schistosomiasis. Schools and water bodies were mapped using a geographical information system. Overall, 34% of children were infected with one or more helminth species whereas 16·2% of children were infected with one or more STH species. Schools in closest proximity to Lake Victoria and River Nyamasaria had the highest S. mansoni prevalence while schools with STH were more homogenously distributed. Mean school prevalence of S. mansoni infection was 21% (range=0-69·7%), S. haematobium 3·6% (range=0-12%), hookworms 6·1% (range=0-20%), A. lumbricoides 4·9% (range=0-18·4%), and T. trichiura 7·7% (range=0-18·6%). Helminth-related morbidities were not associated with infection. Our study demonstrates that schistosomiasis and STH are important health priorities among schools in informal settlements of Kisumu City, and highlights the need for routine deworming in similar settings. 相似文献
862.
Zeck A Pohlentz G Schlothauer T Peter-Katalinić J Regula JT 《Journal of proteome research》2011,10(7):3031-3039
Human leukocyte receptor IIIa (hFcγRIIIa) plays a prominent role in the elimination of tumor cells by antibody-based cancer therapies. In previous studies, a major impact of the presence of carbohydrates at Asn-162 on the binding between the receptor and the Fc part of wild type fucosylated or glycoengineered nonfucosylated antibodies has been shown. In this study, we performed a site directed carbohydrate analysis at hFcγRIIIa derived from human embryonic kidney (HEK) and Chinese hamster ovary (CHO) cells, respectively. Using mass spectrometry (MS) and a multienzyme protein digest, we analyzed the proteolysis-generated glycopeptides in detail. We could show that hFcγRIIIa expressed by HEK cells was mostly bearing multifucosylated biantennary Asn162-glycans with a major fraction terminating with GalNAc residues replacing the more common Gal. We could demonstrate that the glycan antennae with terminal GalNAc could be sialylated as indicated by a novel reporter ion HexNAcHexNAcNeuAc(+) (m/z 698.28) using a source induced dissociation (SID) scan in the MS cycle. In contrast to the hFcγRIIIa Asn-162 glycosylation pattern from HEK cells, the CHO cells derived receptor contains bi- and triantennary galactosylated and highly sialylated carbohydrates. Our data suggest that the type of expression host system was a dominating factor for formation of distinct glycopatterns of hFcγRIIIa, while the protein sequence and the site of glycosylation remained unchanged for both types of cells. Using surface plasmon resonance (SPR) interaction analysis, we show that the cell type and site specific glycosylation pattern of hFcγRIIIa influences its binding behavior to immunoglobulin molecules. 相似文献
863.
Biallelic Mutations in FUT8 Cause a Congenital Disorder of Glycosylation with Defective Fucosylation
864.
865.
K. Skowron N. Wiktorczyk-Kapischke K. Grudelwska-Buda M. Zacharski Z. Paluszak E. Gospodarek-Komkowska 《Letters in applied microbiology》2022,74(4):482-497
The main purpose of micro-organisms elimination from the air and surfaces is to ensure microbiological safety in health care facilities or food production plants. Currently, many disinfection methods are used, both physical, chemical and, increasingly, biological. Scientists seek new solutions with high antimicrobial effectiveness (especially against the drug-resistant strains of bacteria), low production and operating costs, and, above all, the safety of patients and food consumers. The limitation of the methods used so far is primarily the micro-organisms acquire the resistance, mainly to antimicrobial agents. One of the new and alternative methods of disinfection is radiant catalytic ionization (RCI). RCI is an active method of air and surface purification. The technology proved high efficiency against viruses, Gram-positive and -negative bacteria, and fungi, both in the air and on surfaces (planktonic forms and biofilm). RCI has many advantages as well as some minor limitations. This overview summarizes the current knowledge about RCI technology. 相似文献
866.
B. Szajáni Z. Buzás K. Dallmann I. Gimesi J. Krisch M. Tóth 《Applied microbiology and biotechnology》1996,46(2):122-125
Saccharomyces cerevisiae cells were immobilized on preformed cellulose beads by adsorption. The fermentation capacity of the immobilized yeast cells
was found to be practically independent of the hydrogen ion concentration between pH 3.1 and 6.25. The fermentation capacity
was maximal at 30 °C. The immobilized yeast cells were used for continuous production of ethanol in a fluidized-bead reactor.
The average values characteristic for the process were an ethanol concentration of 41.9±0.1 g l-1, a fermentation efficiency of 82.9±2.1% and a volumetric productivity of 3.94±0.52 g l-1 h-1.
Received: 9 October 1995/Accepted: 22 April 1996 相似文献
867.
Björn Cochlovius Volker Zawadzki Astrid Perschl M. Zöller 《Cancer immunology, immunotherapy : CII》1998,46(1):61-66
The adoptive transfer of in vitro generated tumor-specific cytotoxic T lymphocytes (CTL) is considered a promising perspective
in cancer therapy. One possible drawback lies in the inappropriate homing of in vitro cultured lymphocytes, which could be
circumvented by introducing the appropriate targeting molecules. Here we describe a protocol that allows a rapid and stable
transfection of cytotoxic T cell clones. As a model system we used a CTL clone specific for the melanoma-associated antigen
gp100 and a cDNA encoding for murine CD14 containing the variant exen v10 which is supposed to facilitate lymphocyte homing
towards the skin. CD44v10 cDNA was ligated into the retroviral vector pMV-7, which was used to transfect the ecotropic GP-E-86
and the amphotropic PA317 cells. After several cycles of transduction to increase the viral titre, supernatants of the amphotropic
PA317-CD44v10 line were used for transduction of CD44v10 into a human CTL clone. After three cycles of transduction at 12-h
intervals, low but stable expression of CD44v10 was observed throughout the culture period of 10 weeks. The phenotype of the
transduced CTL clone was unaltered and the cytotoxic potential was only slightly reduced as compared to the parental clone.
The efficiency of stable transduction within a period of 1 week makes the protocol well suited for the in vivo transfer of
transduced cells and, in the special case, should guarantee appropriate homing of the transduced CTL clone.
Received: 14 August 1997 / Accepted: 20 November 1997 相似文献
868.
Somatic hybridization of Lycopersicon peruvianum and Petunia hybrida was carried out to transfer cytoplasmic male sterility from Petunia to Lycopersicon. Cytological, morphological and biochemical analyses were performed to characterize the regenerated plants. Two regenerated plants, R3 and R6, were male sterile. R3 possessed chromosomes morphologically similar to those of both parental types. Leaf morphologies of these two plants and a third plant, R7, were intermediate between the two parents. The stability of RUBPCase was verified during parental plant development and after in vitro culture. Plant R7 presented a new form of the large subunit of RUBPCase. 相似文献
869.
A. Wiese M. Münstermann T. Gutsmann B. Lindner K. Kawahara U. Zähringer U. Seydel 《The Journal of membrane biology》1998,162(2):127-138
We have studied the interaction of the polycationic peptide antibiotic polymyxin B (PMB) with asymmetric planar bilayer membranes
via electrical measurements. The bilayers were of different compositions, including those of the lipid matrices of the outer
membranes of various species of Gram-negative bacteria. One leaflet, representing the bacterial inner leaflet, consisted of
a phospholipid mixture (PL; phosphatidylethanolamine, -glycerol, and diphosphatidylglycerol in a molar ratio of 81:17:2).
The other (outer) leaflet consisted either of lipopolysaccharide (LPS) from deep rough mutants of PMB-sensitive (Escherichia coli F515) or -resistant strains (Proteus mirabilis R45), glycosphingolipid (GSL-1) from Sphingomonas paucimobilis IAM 12576, or phospholipids (phosphatidylglycerol, diphytanoylphosphatidylcholine). In all membrane systems, the addition
of PMB to the outer leaflet led to the induction of current fluctuations due to transient membrane lesions. The minimal PMB
concentration required for the induction of the lesions and their size correlated with the charge of the lipid molecules.
In the membrane system resembling the lipid matrix of a PMB-sensitive strain (F515 LPS/PL), the diameters of the lesions were
large enough (d= 2.4 nm ± 8%) to allow PMB molecules to permeate (self-promoted transport), but in all other systems they were too small.
A comparison of these phenomena with membrane effects induced by detergents (dodecyltriphenylphosphonium bromide, dodecyltrimethylammonium
bromide, sodiumdodecylsulfate) revealed a detergent-like mechanism of the PMB-membrane interaction.
Received: 16 September 1997/Revised: 25 November 1997 相似文献
870.
The comparative analysis of the osteometric dimensions of metacarpal bones in three groups of males (aged from 23 to 63 years) differing with respect to the presence of the professionally connected long-term exposure to the vibration is performed. The sample encompasses forest workers employed in wood industry (exposed to heavy physical work and to the daily use of hand-held vibrating tools) from two regions of Croatia: Podravina (n = 192) and Gorski Kotar (n = 115). The control group is formed using the random sample (selected according to age criterion) of phenotypically healthy male inhabitants of rural communities of Eastern Adriatic islands and peninsula (n = 200). Additionally, the influence of the level of calcium in diet, as a regional nutritional characteristic of particular regions of Croatia, is also considered. The regression of the percent cortical area (PCA) of the second left metacarpal bone and age showed that significant decrease of PCA in older age can be observed only in males from Eastern Adriatic (the control group), while that is not so in either group of males professionally exposed to vibration. Authors conclude that the analysis of the osteometric dimensions performed on males professionally daily exposed to vibration missed to provide evidence to support the hypothesis of long-term exposure to vibration as a risk for accelerated osteoporosis of metacarpal bones. 相似文献