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81.
Richard Schwameis Christoph Grimm Edgar Petru Camilla Natter Christine Staudigl Wolfgang Lamm Heinz Koelbl Michael Krainer Thomas Brodowicz Alexander Reinthaller Stephan Polterauer 《PloS one》2015,10(8)
Objective
C-reactive protein (CRP) has previously been shown to serve as a prognostic parameter in women with gynecologic malignancies. Due to the lack of valid prognostic markers for uterine leiomyosarcoma (ULMS) this study set out to investigate the value of pre-treatment CRP serum levels as prognostic parameter.Methods
Data of women with ULMS were extracted from databases of three Austrian centres for gynaecologic oncology. Pre-treatment CRP serum levels were measured and correlated with clinico-pathological parameters. Univariate and multivariable survival analyses were performed.Results
In total, 53 patients with ULMS were included into the analysis. Mean (SD) CRP serum level was 3.46 mg/dL (3.96). Solely, an association between pre-treatment CRP serum levels and tumor size (p = 0.04) but no other clinic-pathologic parameter such as tumor stage (p = 0.16), or histological grade (p = 0.07), was observed. Univariate and multivariable survival analyses revealed that CRP serum levels (HR 2.7 [1.1–7.2], p = 0.037) and tumor stage (HR 6.1 [1.9–19.5], p = 0.002) were the only independent prognostic factors for overall survival (OS) in patients with ULMS. Patients with high pre-treatment CRP serum levels showed impaired OS compared to women with low levels (5-year-OS rates: 22.6% and 52.3%, p = 0.007).Conclusion
High pre-treatment CRP serum levels were independently associated with impaired prognosis in women with ULMS and might serve as a prognostic parameter in these patients. 相似文献82.
The Kirchbach Limestone occurs in the middle part of the early Viséan to Bashkirian Hochwipfel Formation, which was deposited in a flysch basin that formed during an extensional rifting phase in the foreland of the Noric Terrane, was filled with deep-marine synorogenic sediments and closed during the Bashkirian. The Noric Terrane split off from Gondwana and drifted towards the north, closing the flysch basin, which was part of the Paleo-Tethys. The Kirchbach Limestone is composed of bioclastic mudstone and carbonate conglomerate. Microfacies of the limestone clasts include wackestone, packstone, grainstone, and rudstone with diverse fossil assemblages. Bindstone clasts are derived from very shallow, restricted environments. Other clasts are bioclastic mudstone derived from deeper settings. All the foraminifers and algae identified correspond to the upper MFZ14 biozone, after the appearance of Bradyina; in contrast, the markers of the uppermost MFZ14 (Asteroarchaediscus, Loeblichia paraammonoides, and Warnantella) and those of MFZ15 (Janischewskina, Climacammina, and Biseriella) are totally absent. The Kirchbach markers are Cribrospira mikhailovi, Bradyina cf. flosculus, Howchinia bradyana, and Eostaffella parastruvei. Revised local taxa are Mstinia, M. minima n. comb., Consobrinellopsis n. gen., and C. ex gr. consobrina n. comb. The Kirchbach Limestone is derived from a shelf area displaying various shallow-water environments from which the clasts were transported into deeper-marine environments as sediment gravity flows. Limestone clasts of the Kirchbach Limestone indicate the presence of a shallow carbonate shelf along the northern margin of the Hochwipfel flysch basin. The late Asbian (MFZ14) limestone clasts derived from this carbonate shelf were probably subaerially exposed prior to their reworking and redeposition within the flysch sediments, which are late Brigantian (MFZ15) in age. Fossiliferous carbonate shelf sediments of Viséan–Serpukhovian (Namurian) age in the Veitsch Nappe of the eastern Graywacke Zone may be remnants of this shelf. Similar trilobite faunas of Nötsch and Veitsch indicate that they were originally adjacent and probably connected to this shelf north of the flysch basin. These data confirm that the Carnic Alps were located in the Viséan Mediterranean subprovince. 相似文献
83.
SpliceTrap: a method to quantify alternative splicing under single cellular conditions 总被引:1,自引:0,他引:1
Wu J Akerman M Sun S McCombie WR Krainer AR Zhang MQ 《Bioinformatics (Oxford, England)》2011,27(21):3010-3016
84.
85.
N. GARCÍA-MAGALLANES F. LUQUE-ORTEGA E. M. AGUILAR-MEDINA R. RAMOS-PAYÁN C. GALAVIZ-HERNÁNDEZ J. G. ROMERO-QUINTANA L. DEL POZO-YAUNER H. RANGEL-VILLALOBOS E. ARÁMBULA-MERAZ 《Journal of genetics》2014,93(2):325-330
Glucose-6-phosphate dehydrogenase deficiency (G6PD) is the most common enzyme pathology in humans; it is X-linked inherited and causes neonatal hyperbilirubinaemia, chronic nonspherocytic haemolytic anaemia and drug-induced acute haemolytic anaemia. G6PD deficiency has scarcely been studied in the northern region of Mexico, which is important because of the genetic heterogeneity described in Mexican population. Therefore, samples from the northern Mexico were biochemically screened for G6PD-deficiency, and PCR-RFLPs, and DNA sequencing used to identify mutations in positive samples. The frequency of G6PD deficiency in the population was 0.95% (n = 1993); the mutations in 86% of these samples were G6PD A?202A/376G , G6PD A?376G/968C and G6PD Santamaria376G/542T . Contrary to previous reports, we demonstrated that G6PD deficiency distribution is relatively homogenous throughout the country (P = 0.48336), and the unique exception with high frequency of G6PD deficiency does not involve a coastal population (Chihuahua: 2.4%). Analysis of eight polymorphic sites showed only 10 haplotypes. In one individual we identified a new G6PD mutation named Mexico DF193A>G (rs199474830), which probably results in a damaging functional effect, according to PolyPhen analysis. Proteomic impact of the mutation is also described. 相似文献
86.
87.
Thorsten Fuereder Volker Wacheck Sabine Strommer Peter Horak Marion Gerschpacher Wolfgang Lamm Danijel Kivaranovic Michael Krainer 《PloS one》2014,9(4)
Background
Endothelial progenitor cells (CEPs) and circulating endothelial cells (CECs) are potential biomarkers of response to anti-angiogenic treatment regimens. In the current study, we investigated the effect of docetaxel and sunitinib on CEP/CEC kinetics and clinical response in castration resistant prostate cancer (CRPC) patients.Patients and methods
Chemonaive patients with CRPC were enrolled in this study to receive either sunitinib (37.5 mg/d), in combination with docetaxel (75 mg/m2) or docetaxel alone. CEP and CEC kinetics were analyzed for every cycle. The primary objective was to compare CEP/CEC pharmacodynamics between both treatment arms. We also investigated if CEC/CEP spikes, induced by MTD docetaxel, are suppressed by sunitinib in patients treated with docetaxel/sunitinib relative to docetaxel monotherapy.Results
A total of 27 patients were enrolled. We observed a significant increase of CEP/CEC (total/viable) counts over time within each cycle (coefficients 0.29233, 0.22092 and 0.26089, respectively; p<0.001). However, no differences between the treatment groups, in terms of CEP and CEC kinetics, were detected. In the docetaxel monotherapy arm 4 (30%) patients responded to therapy with a 50% PSA decline, while 9 (64%) patients showed a PSA decline in the combination group (n.s.). The median PFS in the docetaxel monotherapy group was 3.1 months (2.6–3.6 months, 95% CI) and 6.2 months (4.9–7.4 months, 95% CI; p = 0.062) in the combination arm. Sunitinib/docetaxel was reasonably well tolerated and toxicity manageable.Conclusion
In summary, no significant differences in CEC and CEP kinetics between the treatment arms were observed, although a highly significant increase of CEPs/CECs within each cycle over time was detected. These results mirror the challenge we have to face when employing anti-angiogenic strategies in CRPC. Additional preclinical research is needed to elucidate the underlying molecular mechanisms. However, docetaxel/sunitinib therapy resulted in a better response in terms of PSA decline and a trend towards improved PFS.Trial Registery
clinicaltrialsregister.eu EudraCT 2007-003705-27 相似文献88.
Pre-mRNA splicing in the new millennium 总被引:39,自引:0,他引:39
The past year has witnessed refinements in models of spliceosome assembly pathways and in the understanding of how splicing factors of the serine/arginine-rich (SR) protein family function. The role of splicing in human genetic diseases has also received a lot of attention recently as exonic splicing enhancers become better understood. 相似文献
89.
Florian W Krainer Christian Dietzsch Tanja Hajek Christoph Herwig Oliver Spadiut Anton Glieder 《Microbial cell factories》2012,11(1):22
Βackground
The methylotrophic yeast Pichia pastoris has become an important host organism for recombinant protein production and is able to use methanol as a sole carbon source. The methanol utilization pathway describes all the catalytic reactions, which happen during methanol metabolism. Despite the importance of certain key enzymes in this pathway, so far very little is known about possible effects of overexpressing either of these key enzymes on the overall energetic behavior, the productivity and the substrate uptake rate in P. pastoris strains. 相似文献90.
Lucas Bleicher Ricardo Aparicio Fabio M Nunes Leandro Martinez Sandra M Gomes Dias Carolina Migliorini Ana Figueira Auxiliadora Morim Maria Santos Walter H Venturelli Rosangela da Silva Paulo Marcos Donate Francisco AR Neves Luiz A Simeoni John D Baxter Paul Webb Munir S Skaf Igor Polikarpov 《BMC structural biology》2008,8(1):1-13