Prokaryotic Diversity in the Biotopes of the Gudzhirganskoe Saline Lake (Barguzin Valley,Russia)

Microbiology - The hydrochemical water composition and the mineralogical composition of microbial mats and bottom sediments were determined for the ephemeral saline soda lake Gudzhirganskoe...     

Prevalence of human papillomavirus infection,distribution of viral types and risk factors in cervical samples from human immunodeficiency virus-positive women attending three human immunodeficiency virus-acquired immune deficiency syndrome reference centres in northeastern Brazil

Human immunodeficiency virus (HIV)-positive patients have a greater prevalence of coinfection with human papillomavirus (HPV) is of high oncogenic risk. Indeed, the presence of the virus favours intraepithelial squamous cell lesion progression and may induce cancer. The aim of this study was to evaluate the prevalence of HPV infection, distribution of HPV types and risk factors among HIV-positive patients. Cervical samples from 450 HIV-positive patients were analysed with regard to oncotic cytology, colposcopy and HPV presence and type by means of polymerase chain reaction and sequencing. The results were analysed by comparing demographic data and data relating to HPV and HIV infection. The prevalence of HPV was 47.5%. Among the HPV-positive samples, 59% included viral types of high oncogenic risk. Multivariate analysis showed an association between HPV infection and the presence of cytological alterations (p = 0.003), age greater than or equal to 35 years (p = 0.002), number of partners greater than three (p = 0.002), CD4⁺ lymphocyte count < 200/mm³ (p = 0.041) and alcohol abuse (p = 0.004). Although high-risk HPV was present in the majority of the lesions studied, the low frequency of HPV 16 (3.3%), low occurrence of cervical lesions and preserved immunological state in most of the HIV-positive patients were factors that may explain the low occurrence of precancerous cervical lesions in this population.     

Single-nucleotide polymorphism <Emphasis Type="Italic">rs</Emphasis>11562975 of the thermosensitive ion channel <Emphasis Type="Italic">TRPM</Emphasis>8 gene and human sensitivity to cold and menthol

The examination of subjects from the Russian ethnic group revealed that 20.3% of them had the heterozygous genotype containing the C-allele in the rs11562975 (G → C) single-nucleotide polymorphism located in exon 7 of the gene encoding the temperature-sensitive ion channel TRPM8. Functional differences associated with the sensitivity to cold and menthol were revealed in subjects with different rs11562975 polymorphism genotypes (GG and GC). The subjects with the heterozygous genotype GC were characterized by increased sensitivity to cold and decreased sensitivity to menthol, an agonist of the ion channel TRPM8, compared to the subjects with the homozygous genotype GG     

Kinetics of uranous iron and ferrous iron oxidation by thiobacillus ferrooxidans

Archives of Microbiology -     

Respiratory system response to local cooling in subjects with single nucleotide polymorphism rs11562975 of the TRPM8 temperature-sensitive ion channel gene

Genome studies in ethnic Russians revealed subjects having a heterozygous genotype with allele C at the single-nucleotide polymorphism rs11562975 (G → C), which is in exon 6 of the TRPM8 temperature-sensitive ion channel gene. The subjects had increased sensitivity to cold and produced a hypometabolic response to local skin cooling and to temperature-independent activation of the TRPM8 ion channel by menthol, including a decrease in gas exchange, pulmonary ventilation, and oxygen extraction coefficient. Subjects with the homozygous genotype GG had a lower sensitivity to cold, and their response to local skin cooling was adequate in terms of thermoregulation, including a decrease in respiratory heat loss and an increase in lipid metabolism.     

The inhibition of translation in Bacillus subtilis via the repression by the secreted enzyme of its synthesis]

It has been shown that inhibition of Bacillus subtilis alpha-amylase formation by the level of the active enzyme in the cultural medium leads to the decrease of translation accompanied by reduction of level of [14C] valin transport into the cells.