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排序方式: 共有126条查询结果,搜索用时 16 毫秒
81.
N. A. Kozub I. A. Sozinov T. A. Sobko V. T. Kolyuchii S. V. Kuptsov A. A. Sozinov 《Cytology and Genetics》2009,43(1):55-62
Genotypes at the gliadin loci Gli-A1, Gli-B1, Gli-D1 and the high-molecular-weight glutenin subunit loci Glu-A1, Glu-B1, Glu-D1 were identified in 77 winter common wheat cultivars developed in the Central Forest Steppe of Ukraine in different periods
of time. The highest level of variation was observed at the Gli-A1 locus. Predominant alleles (one or two per locus) were revealed. The comparison of allele frequencies in groups of cultivars
developed in different periods of time (before 1996 and in 1996–2007) has demonstrated appearance of new alleles and change
of frequencies of existing alleles at the storage protein loci. The high frequency of cultivars with the wheat-rye 1BL/1RS
translocation was detected (about 40%). The wheat rye 1AL/1RS translocation was identified in six cultivars developed in the
last decade. Four gliadin alleles, Gli-A1w (a marker for the 1AL/1RS translocation), Gli-A1x, Gli-A1y and Gli-B1x, were proposed for cataloging.
The article is published in the original. 相似文献
82.
Szklarczyk R Wanschers BF Cuypers TD Esseling JJ Riemersma M van den Brand MA Gloerich J Lasonder E van den Heuvel LP Nijtmans LG Huynen MA 《Genome biology》2012,13(2):R12-14
Background
Orthology is a central tenet of comparative genomics and ortholog identification is instrumental to protein function prediction. Major advances have been made to determine orthology relations among a set of homologous proteins. However, they depend on the comparison of individual sequences and do not take into account divergent orthologs.Results
We have developed an iterative orthology prediction method, Ortho-Profile, that uses reciprocal best hits at the level of sequence profiles to infer orthology. It increases ortholog detection by 20% compared to sequence-to-sequence comparisons. Ortho-Profile predicts 598 human orthologs of mitochondrial proteins from Saccharomyces cerevisiae and Schizosaccharomyces pombe with 94% accuracy. Of these, 181 were not known to localize to mitochondria in mammals. Among the predictions of the Ortho-Profile method are 11 human cytochrome c oxidase (COX) assembly proteins that are implicated in mitochondrial function and disease. Their co-expression patterns, experimentally verified subcellular localization, and co-purification with human COX-associated proteins support these predictions. For the human gene C12orf62, the ortholog of S. cerevisiae COX14, we specifically confirm its role in negative regulation of the translation of cytochrome c oxidase.Conclusions
Divergent homologs can often only be detected by comparing sequence profiles and profile-based hidden Markov models. The Ortho-Profile method takes advantage of these techniques in the quest for orthologs. 相似文献83.
Hofmanová J Ciganek M Slavík J Kozubík A Stixová L Vaculová A Dušek L Machala M 《The Journal of nutritional biochemistry》2012,23(6):539-548
The present study highlights the important association between lipid alterations and differentiation/apoptotic responses in human colon differentiating (FHC) and nondifferentiating (HCT-116) cell lines after their treatment with short-chain fatty acid sodium butyrate (NaBt), polyunsaturated fatty acids (PUFAs), and/or their combination. Our data from GC/MS and LC/MS/MS showed an effective incorporation and metabolization of the supplemented arachidonic acid (AA) or docosahexaenoic acid (DHA), resulting in an enhanced content of the respective PUFA in individual phospholipid (PL) classes and an altered composition of the whole cellular fatty acid spectrum in both FHC and HCT-116 cells. We provide novel evidence that NaBt combined with PUFAs additionally modulated AA and DHA cellular levels and caused their shift from triacylglycerol to PL fractions. NaBt increased, while AA, DHA and their combination with NaBt decreased endogenous fatty acid synthesis in FHC but not in HCT-116 cells. Fatty acid treatment also altered membrane lipid structure, augmented cytoplasmic lipid droplet accumulation, reactive oxygen species (ROS) production and dissipation of the mitochondrial membrane potential. All these parameters were significantly enhanced by combined NaBt/PUFA treatment, but only in FHC cells was this accompanied by highly increased apoptosis and suppressed differentiation. Moreover, the most significant changes of ROS production, differentiation and apoptosis among the parameters studied, the highest effects of combined NaBt/PUFA treatment and a lower sensitivity of HCT-116 cells were confirmed using two-way ANOVA. Our results demonstrate an important role of fatty acid-induced lipid alterations in the different apoptotic/differentiation response of colon cells with various carcinogenic potential. 相似文献
84.
85.
Andrea Staršíchová Lukáš Kubala Eva Lincová Zuzana Pernicová Alois Kozubík Karel Souček 《Biological procedures online》2009,11(1):316-324
The plasticity of differentiated adult cells could have a great therapeutic potential, but at the same time, it is characteristic
of progression of serious pathological states such as cancer and fibrosis. In this study, we report on the application of
a real-time noninvasive system for dynamic monitoring of cellular plasticity. Analysis of the cell impedance profile recorded
as cell index using a real-time cell analyzer revealed its significant increase after the treatment of prostate epithelial
cells with the transforming growth factor-β1. Changes in the cell index profile were paralleled with cytoskeleton rebuilding
and induction of epithelial–mesenchymal transition and negatively correlated with cell proliferation. This novel application
of such approach demonstrated a great potential of the impedance-based system for noninvasive and real-time monitoring of
cellular fate. 相似文献
86.
The effects of parenteral lipid emulsions on cancer and normal human colon epithelial cells in vitro
Hofmanová J Zadák Z Hyspler R Mikeska J Zdánský P Vaculová A Netíková J Kozubík A 《Physiological research / Academia Scientiarum Bohemoslovaca》2005,54(4):409-418
Differences in lipid metabolism of tumor and normal tissues suggest a distinct response to available lipid compounds. In this study, the in vitro effects of five types of commercial parenteral lipid emulsions were investigated on human cell lines derived from normal fetal colon (FHC) or colon adenocarcinoma (HT-29). Changes of the cellular lipid fatty acid content, cell oxidative response, and the cell growth and death rates were evaluated after 48 h. No effects of any type of emulsions were detected on cell proliferation and viability. Compared to the controls, supplementation with lipid emulsions resulted in a multiple increase of linoleic and linolenic acids in total cell lipids, but the content of arachidonic, eicosapentaenoic, and docosahexaenoic acids decreased particularly in HT-29 cells. The concentration of emulsions which did not affected HT-29 cells increased the percentage of floating and subG0/G1 FHC cells probably due to their higher reactive oxygen species production and lipid peroxidation. Co-treatment of cells with antioxidant Trolox reduced the observed effects. Our results imply that lipid emulsions can differently affect the response of colon cells of distinct origin. 相似文献
87.
Effect of an introgression from Aegilops cylindrica host on manifestation of productivity traits in winter common wheat F2 plants 总被引:1,自引:0,他引:1
The effect of introgression of a chromosome 1D segment from Aegilops cylindrica to winter common wheat on productivity traits in F2 plants was studied using storage protein loci as genetic markers. An allele of the gliadin-coding Gli-D1 locus served as a marker of the introgression. Using of two- and three-locus interaction models, it was shown that the introgression tagged with Gli-D1 affected the manifestation of productivity traits (productive tillering, grain weight per plant and grain number per plant) through interaction with other marker storage protein loci: Glu-B1, Glu-D1, and Gli-B2. 相似文献
88.
Segregation at the Gli-B1 locus was studied in F2 seeds of common wheat from crosses between near-isogenic lines with respect to this locus. Segregations differed from the expected ratio in hybrids involving the lines with the allele Gli-B1l (Gli-B1-3), which is a marker for the 1BL/1RS translocation, as well as in the hybrid between the lines with the alleles Gli-B1b (1) and Gli-B1e (4). Reduced transmission of the chromosome with the 1BL/1RS translocation through pollen was observed in the hybrids involving the line with this translocation. In the cross GLI-B1-1 x GLI-B1-4, the significantly lower frequency of female gametes with the allele Gli-B1e (4) was detected. This is due to linkage of the Gli-B1 locus to a factor responsible for segregation distortion in female gametes. We proposed to designate this locus Sd3. The line with the gliadin block Gli-B1e differs in alleles at the Sd3 locus from the lines with the blocks Gli-B1b and Gli-B1o. 相似文献
89.
90.
Asensio JL; Canada FJ; Bruix M; Gonzalez C; Khiar N; Rodriguez-Romero A; Jimenez-Barbero J 《Glycobiology》1998,8(6):569-577
The specific interaction of hevein with GlcNAc-containing oligosaccharides
has been analyzed by1H-NMR spectroscopy. The association constants for the
binding of hevein to a variety of ligands have been estimated from1H-NMR
titration experiments. The association constants increase in the order
GlcNAc-alpha(1-->6)-Man < GlcNAc < benzyl-beta-GlcNAc <
p-nitrophenyl-beta-GlcNAc < chitobiose < p-
nitrophenyl-beta-chitobioside < methyl-beta-chitobioside <
chitotriose. Entropy and enthalpy of binding for different complexes have
been obtained from van't Hoff analysis. The driving force for the binding
process is provided by a negative DeltaH0which is partially compensated by
negative DeltaS0. These negative signs indicate that hydrogen bonding and
van der Waals forces are the major interactions stabilizing the complex.
NOESY NMR experiments in water solution provided 475 accurate protein
proton-proton distance constraints after employing the MARDIGRAS program.
In addition, 15 unambiguous protein/carbohydrate NOEs were detected. All
the experimental constraints were used in a refinement protocol including
restrained molecular dynamics in order to determine the highly refined
solution conformation of this protein- carbohydrate complex. With regard to
the NMR structure of the free protein, no important changes in the protein
nOe's were observed, indicating that carbohydrate-induced conformational
changes are small. The average backbone rmsd of the 20 refined structures
was 0.055 nm, while the heavy atom rmsd was 0.116 nm. It can be deduced
that both hydrogen bonds and van der Waals contacts confer stability to the
complex. A comparison of the three-dimensional structure of hevein in
solution to those reported for wheat germ agglutinin (WGA) and hevein
itself in the solid state has also been performed. The polypeptide
conformation has also been compared to the NMR-derived structure of a
smaller antifungical peptide, Ac-AMP2.
相似文献