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Restriction fragment length variants have been exploited to map genetically Rb-1, the murine homolog of the human retinoblastoma gene. Rb-1 localized to mouse chromosome 14 on the basis of results from analysis of somatic cell hybrids. In an interspecific backcross involving Mus spretus, Rb-1 and the murine homolog of the human esterase D gene (ESD), which we refer to here as Esd, were inseparable. Furthermore, the strain distribution patterns of Rb-1 and Es-10 are the same in 31 of 32 recombinant inbred strains. Close linkage of the chromosome 14 morphological marker hairless (hr) to Rb-1 is also implied. These results localize Rb-1 on the mouse linkage map and provide close genetic markers to follow Rb-1 in somatic as well as in germline genetic experiments. Additionally, the results suggest that Es-10 is the murine homolog of ESD and provide further evidence for linkage conservation during mammalian evolution. 相似文献
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W Kozak G Chesy W Kadziela M Caputa A Lachowski 《Comparative biochemistry and physiology. A, Comparative physiology》1985,81(1):165-169
Intravenous injection of E. coli LPS (0.5 micrograms/kg) produced the biphasic elevation of rectal temperature (TR) in conscious oxen. The fever was accompanied by a significant increase of the arylsulphatase A (AsA) activities in plasma and in mononuclear leucocytes. In polymorphonuclear cells a substantial decrease of the AsA activity after 1 hr fever was observed. After 3.5 hr of fever the polymorphonuclear activity of AsA restored to normal found before LPS administration. In contrast with AsA, the pyrogenic dose of LPS caused negligible changes of the acid phosphatase (AcP) activities in the sampled materials. Daily-repeated injections of pyrogen into the same oxen attenuated magnitudes of fever as well as AsA responses in plasma and granulocytes. Heat-induced hyperthermia provoked only minute changes of the AsA and AcP. 相似文献
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Qian Yuan Christine A. Kozak Wei-meng Jing Daniel Hollander James D. Watson Geoffrey W. Krissansen 《Immunogenetics》1992,35(6):403-407
Address correspondence and offprint requests to: G. W. Krissansen. 相似文献
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Using mouse probes specific to cytochrome cS and to cytochrome cT, the single-copy genes encoding these two proteins have been mapped to paralogous chromosomal regions by analysis of restriction fragment length variants in interspecific crosses. The gene for cytochrome cS, Cycs, maps to a position between Tcrb and Cbl-1 on proximal mouse Chromosome 6, and the gene for cytochrome cT, Cyct, maps between Gad-1 and Sfpi-1 on mouse Chromosome 2. 相似文献
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