全文获取类型
收费全文 | 652篇 |
免费 | 132篇 |
国内免费 | 9篇 |
出版年
2021年 | 4篇 |
2020年 | 7篇 |
2019年 | 10篇 |
2018年 | 4篇 |
2017年 | 6篇 |
2016年 | 11篇 |
2015年 | 25篇 |
2014年 | 25篇 |
2013年 | 21篇 |
2012年 | 34篇 |
2011年 | 28篇 |
2010年 | 28篇 |
2009年 | 18篇 |
2008年 | 24篇 |
2007年 | 20篇 |
2006年 | 23篇 |
2005年 | 23篇 |
2004年 | 13篇 |
2003年 | 21篇 |
2002年 | 25篇 |
2001年 | 22篇 |
2000年 | 20篇 |
1999年 | 26篇 |
1998年 | 15篇 |
1997年 | 17篇 |
1996年 | 21篇 |
1995年 | 19篇 |
1994年 | 19篇 |
1993年 | 13篇 |
1992年 | 27篇 |
1991年 | 26篇 |
1990年 | 20篇 |
1989年 | 22篇 |
1988年 | 10篇 |
1987年 | 21篇 |
1986年 | 13篇 |
1985年 | 18篇 |
1984年 | 11篇 |
1983年 | 8篇 |
1982年 | 7篇 |
1981年 | 4篇 |
1980年 | 4篇 |
1979年 | 11篇 |
1978年 | 5篇 |
1977年 | 8篇 |
1975年 | 4篇 |
1974年 | 7篇 |
1973年 | 5篇 |
1972年 | 4篇 |
1969年 | 3篇 |
排序方式: 共有793条查询结果,搜索用时 31 毫秒
11.
12.
M Danciger C A Kozak T Abe T Shinohara D B Farber 《Cytogenetics and cell genetics》1991,56(3-4):202-205
A water-soluble protein (called "33-kDa protein") that exhibits light-dependent phosphorylation has been shown to be a major protein of mammalian rod photoreceptors. Although the function of this protein is unknown, it has been implicated in the biochemical cascade mediating the rod visual response. Using a retinal cDNA from the rat and somatic cell hybrids, we have mapped the gene corresponding to this protein to mouse Chromosome 1 and, by analyzing the progeny of an intersubspecific backcross, have positioned it near Lamb2 (the beta 2 chain of laminin). We have designated the gene Rpr-1 (rod photoreceptor protein-1). 相似文献
13.
Common proviral integration region on mouse chromosome 7 in lymphomas and myelogenous leukemias induced by Friend murine leukemia virus. 总被引:18,自引:12,他引:6
下载免费PDF全文
![点击此处可从《Journal of virology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Friend murine leukemia virus (F-MuLV) induces a variety of hematopoietic neoplasms 2 to 12 months after inoculation into newborn mice. These neoplasms are clonal or oligoclonal and contain a small number of F-MuLV insertions in high-molecular-weight DNA. To investigate whether different tumors have proviral insertions in the same region, a provirus-cellular DNA junction fragment from an F-MuLV-induced myelogenous leukemia was cloned in lambda gtWES, and a portion of the flanking cellular DNA sequence was used in blot-hybridization studies of 34 additional F-MuLV-induced neoplasms. Three of these additional neoplasms (one myelogenous leukemia and two lymphomas) were found to have altered copies of the flanking cellular sequence. Restriction enzyme analysis of genomic DNA from these tumors revealed that in each case a proviral copy of F-MuLV had inserted into the same 1.5-kilobase region; all proviruses had the same orientation. Using mouse-Chinese hamster somatic cell hybrids, we mapped this common integration region, designated Fis-1, to mouse chromosome 7. Fis-1 is distinct from three oncogenes on mouse chromosome 7, Ha-ras, fes, and Int-2, based on restriction enzyme analysis and blot hybridization. Therefore, Fis-1 appears to be a novel sequence implicated in both lymphoid and myeloid leukemias induced by F-MuLV. 相似文献
14.
Nonecotropic murine leukemia viruses in BALB/c and NFS/N mice: characterization of the BALB/c Bxv-1 provirus and the single NFS endogenous xenotrope 总被引:10,自引:8,他引:2
下载免费PDF全文
![点击此处可从《Journal of virology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
We used hybridization probes that react specifically with xenotropic and mink cell focus-forming virus envelope sequences to characterize the nonecotropic proviruses of BALB/c and NFS/N mice. Analysis of somatic cell hybrids with different BALB/c chromosomes showed that the 9 xenotropic and more than 20 MCF virus-related proviral sequences in this mouse were present on more than nine BALB/c chromosomes. Multiple copies were found on chromosomes 1, 4, 7, 12, and probably 11, and the copies found on a single chromosome were not identical by restriction enzyme mapping. We also identified and characterized the proviral sequences that give rise to infectious xenotropic virus in both BALB/c and NFS/N mice. BALB/c contains the major locus for induction of infectious virus in inbred mice, Bxv-1, which is on chromosome 1. We showed that this locus contains a single xenotropic provirus on an 18-kilobase HindIII fragment. Restriction enzyme analysis of a hybrid cell DNA that contains only the Bxv-1 xenotropic provirus showed that the Bxv-1 provirus contains restriction enzyme sites characteristic of the infectious virus induced from BALB/c fibroblasts. The Bxv-1 provirus and its flanking sequences also contain the same restriction sites as the provirus thought to contribute U3 long terminal repeat sequences to leukemogenic (class I) AKR MCF viruses. Analysis of cell hybrids made with the nonvirus-inducible strain NFS/N showed that the single xenotropic virus env gene of NFS mice, here termed Nfxv-1, is not on chromosome 1. Unlike that of Bxv-1, the restriction map of Nfxv-1 does not resemble that of any known infectious xenotropic virus including xenotropic viruses isolated from NFS mice. These data suggest that Bxv-1, but not Nfxv-1, is a full-length xenotropic provirus that can be transcribed directly to produce infectious virus. 相似文献
15.
Bilateral lesions of basomedial amygdaloid nuclei are capable of significantly inhibiting muricidal aggression induced by oral p-chlorophenylalanine (p-CPA) in male rats. Rats lesioned in extra-amygdaloid structures or sham-lesioned show the usual p-CPA-induced muricidal activity, which ranges from 70 to 80% of treated animals. The results obtained indicate that basomedial amygdaloid nuclei play an important role in regulating p-CPA-induced muricidal aggression, even though the effect lasts for a relatively limited period of time. This fact is probably due to the intervention of still unidentified compensatory mechanisms. 相似文献
16.
C A Kozak 《Journal of virology》1984,51(3):876-879
The time course of murine leukemia virus production after chemical induction was determined in hamster-mouse somatic cell hybrids containing the xenotropic murine leukemia virus induction locus Bxv-1 or the ecotropic locus Akv-2. By using these hybrids, induction could be studied in the absence of secondary virus spread because xenotropic viruses cannot infect hybrid cells and ecotropic viruses cannot infect hybrids which have lost mouse chromosome 5. After induction, hybrids with Bxv-1 produced only a transient burst of virus, whereas those with Akv-2 continued to produce virus for periods in excess of 3 months. The presence or absence of other mouse chromosomes in the hybrid lines did not alter these induction patterns. Thus, endogenous murine leukemia virus loci differ in their response to induction, and both inducibility and the kinetics of virus expression are controlled at or near these proviral loci. 相似文献
17.
Two genetically transmitted BALB/c mouse mammary tumor virus genomes located on chromosomes 12 and 16. 总被引:13,自引:10,他引:3
下载免费PDF全文
![点击此处可从《Journal of virology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
We have examined EcoRI-restricted cellular DNA from BALB/c mouse-hamster somatic cell hybrids by blot hybridization for the presence of mouse mammary tumor virus-related sequences. Results of this analysis show that mouse mammary tumor virus-related proviral copies are located on chromosomes 16 (16-kilobase-pair fragment) and 12 (10.5- and 7.7-kilobase-pair fragments). 相似文献
18.
Hardies SC; Martin SL; Voliva CF; Hutchison CA d; Edgell MH 《Molecular biology and evolution》1986,3(2):109-125
19.
Genetic mapping of a mouse chromosomal locus required for mink cell focus-forming virus replication. 总被引:19,自引:12,他引:7
下载免费PDF全文
![点击此处可从《Journal of virology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
C A Kozak 《Journal of virology》1983,48(1):300-303
Mouse-hamster somatic cell hybrids were used to show that the recombinant mink cell focus-forming murine leukemia viruses and their ecotropic virus progenitors require different mouse chromosomes for replication. Mouse chromosome 1 was shown to carry the genetic information necessary for the replication of six different mink cell focus-forming isolates, and this gene, designated Rmc-1, was tentatively positioned at the distal end of the chromosome. 相似文献
20.
Translation of insulin-related polypeptides from messenger RNAs with tandemly reiterated copies of the ribosome binding site 总被引:25,自引:0,他引:25
Plasmids have been constructed containing reiterated copies of a 66 bp fragment, loosely referred to as the ribosome binding site, that includes the AUG initiator codon of preproinsulin. The extreme test involved plasmid 255/17, which carried four tandem copies of the ribosome binding site, with all four AUG triplets in the same reading frame as the preproinsulin coding sequence downstream. Initiation at any potential start site would generate a polypeptide precipitable with anti-insulin antiserum, and its size would reveal the AUG(s) active in initiation. One insulin-related polypeptide was synthesized in cells transfected by p255/17; its size corresponded to the product initiated at the first ribosome binding site in the tandem array. Inasmuch as the three downstream AUG triplets, which are not used, occur in a sequence context identical with that around the 5'-proximal AUG triplet, which is used, the position of an AUG triplet relative to the 5' end of the mRNA appears to be important in identifying it as a functional initiator codon. 相似文献