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131.
Jin-Beom Si Eun-Ju Jang Dimitris Charalampopoulos Young-Jung Wee 《Biotechnology and Bioprocess Engineering》2018,23(2):176-182
An ammonium sulfate precipitation of fermentation broth produced by Bacillus subtilis FBL-1 resulted in 2.9-fold increase of specific protease activity. An eluted protein fraction from the column chromatographies using DEAE-Cellulose and Sephadex G-75 had 94.2- and 94.9-fold higher specific protease activity, respectively. An SDS-PAGE revealed a band of purified protease at approximately 37.6 kDa. Although purified protease showed the highest activity at 45°C and pH 9.0, the activity remained stable in temperature range from 30 to 50°C and pH range from 7.0 to 9.0. Protease activity was activated by metal ions such as Ca2+, Mg2+, Mn2+, Fe2+, Ca2+ and K+, but 10 mM Fe3+ significantly inhibited enzyme activity (53%). Protease activity was inhibited by 2 mM EDTA as a metalloprotease inhibitor, but it showed good stability against surfactants and organic solvents. The preferred substrates for protease activity were found to be casein (100%) and soybean flour (71.6%). 相似文献
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John Matsoukas Morley D. Hollenberg Thomas Mavromoustakos Dimitris Panagiotopoulos Kostas Alexopoulos Raguav Yamdagni Qiao Wu Graham J. Moore 《The protein journal》1997,16(2):113-131
The conformational properties of the pentapeptide Ser-Phe-Leu-Leu-Arg (P5), a human thrombin receptor-derived sequence forming part of a tethered ligand which activates the thrombin receptor, and its more active amide derivative Ser-Phe-Leu-Leu-Arg-NH2 (P5-NH2), have been studied by proton NMR spectroscopy in dimethylsulfoxide. Measurements of nuclear Overhauser effects, performed using two-dimensional rotating frame nuclear Overhauser (ROESY) and one-dimensional nuclear Overhauser enhancement (NOE) spectroscopy, revealed that P5 exists mainly in an extended conformation. However, proton–proton 1D-NOEs between Phe CαH and Ser CαH, Leu3 CαH and Leu3 NH, and Leu4 CαH and Leu4 NH, as well as between the Ser and Arg sidechains, also implicated a minor conformer for P5 having a curved backbone and a near-cyclic structure. In contrast to P5, measurements of NOEs and ROEs for P5-NH2 revealed a more stabilized cyclic structure which may account for its higher biological potency. Thus strong interresidue sequential NH (i)–NH (i + 1) interactions, as well as C-terminal carboxamide to N-terminal side-chain interactions, i.e., Arg CONH2 to Phe ring and Arg CONH2 to Ser $C_\alpha /C_{\beta \beta '} $ , observed at lower levels of the ROESY spectrum, supported a curved backbone structure for SFLLR-NH2. Since the higher potaency P5-NH2 analogue adopts predominantly a cyclic structure, a cyclic bioactive conformation for thrombin receptor agonist peptides is suggested. 相似文献
134.
Maria Keramida John M. Matsoukas Dimitris Panagiotopoulos Kostas Alexopoulos Elizabeth Matsoukas John Cladas Vasilis A. Tzingounis Emmanuel Cardamakis Hernâni L. S. Maia Debanaba Pati Hamid R. Habibi 《Letters in Peptide Science》1996,3(4):257-262
Summary Gonadotropin-releasing hormone (GnRH) stimulates the release and synthesis of gonadotropin hormones (GtH) and is the key regulator of reproduction. The present study was carried out to design a potent GnRH analogue containing Tyr(OMe) at position 5 and ad-amino acid at position 6. This was based on a previous study in which [Tyr(OMe)5]GnRH was shown to have reduced potency compared to GnRH. A novel GnRH peptide containing Tyr(OMe)5 andd-Glu6 in combination with other substitutions at positions 9 and 10 was synthesized in the present study and tested for binding to the rat pituitary as well as potency in terms of gonadotropin (GtH) release in the goldfish pituitary and ovulation in sea bass. The results demonstrate that the replacement of the glycine residue at position 6 with ad-Glu in combination with the substitution of proline at position 9 with azetidine (Aze) increased the binding and biological activity of [Tyr(OMe)5]GnRH. The observed increased potency is likely to be related to the improved resistance to degradation. The present findings may lead to the development of a more potent GnRH agonist for inducing ovulation in fish. 相似文献
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136.
Dimitris Varvaki Rados Lana Catani Pinto Luciana Reck Remonti Cristiane Bauermann Leit?o Jorge Luiz Gross 《PLoS medicine》2016,13(4)
BackgroundSulfonylureas are an effective and inexpensive treatment for type 2 diabetes.
There is conflicting data about the safety of these drugs regarding
mortality and cardiovascular outcomes. The objective of the present study
was to evaluate the safety of the sulfonylureas most frequently used and to
use trial sequential analysis (TSA) to analyze whether the available sample
was powered enough to support the results.ConclusionsSulfonylureas are not associated with increased risk for all-cause mortality,
cardiovascular mortality, myocardial infarction, or stroke. Current evidence
supports the safety of sulfonylureas; an absolute risk of 0.5% could be
firmly discarded.
Review registration
PROSPERO CRD42014004330 相似文献137.
Catherine Koukoulitsa Caroline Villalonga-Barber Robert Csonka Xanthippi Alexi Georgios Leonis Dimitris Dellis 《Journal of enzyme inhibition and medicinal chemistry》2016,31(1):67-77
It has been reported that beta amyloid induces production of radical oxygen species and oxidative stress in neuronal cells, which in turn upregulates β-secretase (BACE-1) expression and beta amyloid levels, thereby propagating oxidative stress and increasing neuronal injury. A series of resveratrol derivatives, known to be inhibitors of oxidative stress-induced neuronal cell death (oxytosis) were biologically evaluated against BACE-1 using homogeneous time-resolved fluorescence (TRF) assay. Correlation between oxytosis inhibitory and BACE-1 inhibitory activity of resveratrol derivatives was statistically significant, supporting the notion that BACE-1 may act as pivotal mediator of neuronal cell oxytosis. Four of the biologically evaluated resveratrol analogs demonstrated considerably higher activity than resveratrol in either assay. The discovery of some “hits” led us to initiate detailed docking studies associated with Molecular Dynamics in order to provide a plausible explanation for the experimental results and understand their molecular basis of action. 相似文献
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Despoina Miltiadou Ouranios Tzamaloukas Nicholas Genethliou Christakis Papachristoforou Dimitris Chatziplis Michael Orford 《Small Ruminant Research》2010,88(1):72-74
To assess whether established single nucleotide polymorphisms (SNPs) affecting bovine milk traits are present in small ruminants, we genotyped samples from Chios and Cyprus fat-tailed sheep, as well as Damascus and Machaeras goats for the presence of the acylCoA:diacylglycerol acyltransferase 1 (DGAT1) K232A and the growth hormone receptor (GHR) F279Y SNPs. Both of these SNPs have been previously well documented in cattle as having strong effects on milk yield and composition and have been postulated as being the causative mutations to partially explain QTLs identified on bovine chromosomes 14 and 20. Although we were able to confirm the presence of both of these mutations in bovine sample controls by both allele specific PCR reactions and direct DNA sequencing, we were unable to detect them in any of the four major pure breeds of sheep and goats supporting the dairy industry of Cyprus. 相似文献