全文获取类型
收费全文 | 346篇 |
免费 | 21篇 |
出版年
2022年 | 3篇 |
2021年 | 2篇 |
2020年 | 2篇 |
2019年 | 3篇 |
2018年 | 6篇 |
2017年 | 6篇 |
2016年 | 5篇 |
2015年 | 11篇 |
2014年 | 14篇 |
2013年 | 12篇 |
2012年 | 20篇 |
2011年 | 20篇 |
2010年 | 11篇 |
2009年 | 10篇 |
2008年 | 10篇 |
2007年 | 7篇 |
2006年 | 11篇 |
2005年 | 15篇 |
2004年 | 12篇 |
2003年 | 10篇 |
2002年 | 12篇 |
2001年 | 16篇 |
2000年 | 8篇 |
1999年 | 11篇 |
1998年 | 8篇 |
1997年 | 10篇 |
1996年 | 8篇 |
1995年 | 4篇 |
1994年 | 8篇 |
1993年 | 4篇 |
1992年 | 8篇 |
1991年 | 7篇 |
1990年 | 5篇 |
1989年 | 5篇 |
1988年 | 5篇 |
1987年 | 7篇 |
1986年 | 2篇 |
1985年 | 3篇 |
1984年 | 10篇 |
1983年 | 4篇 |
1982年 | 4篇 |
1981年 | 3篇 |
1979年 | 3篇 |
1978年 | 3篇 |
1975年 | 3篇 |
1974年 | 2篇 |
1969年 | 1篇 |
1968年 | 5篇 |
1965年 | 1篇 |
1963年 | 1篇 |
排序方式: 共有367条查询结果,搜索用时 46 毫秒
11.
T. Yanagihara 《Journal of neurochemistry》1979,32(1):169-177
Protein and RNA syntheses were investigated with bulk isolated nerve and glial cells from rabbit brain. For polypeptide synthesis, ‘intact’ cells were incubated with [3H]leucine under various conditions and the results were compared with those of polyribosomal polypeptide synthesis. For RNA synthesis ‘intact’ cells were incubated with [3H]uridine or [3H]guanosine and the results were compared with those of DNA-dependent RNA polymerase assay. The bulk isolated ‘intact’ nerve cells were more active in protein synthesis than the ‘intact’ glial cells, while the latter synthesized RNA more actively than the former, although both polyribosomal polypeptide synthesis and DNA-dependent RNA polymerase activity were higher with the nerve cells, indicating a higher potential for the nerve cells. The observed discrepancy of RNA synthesis was explained by the significantly less active uptake of nucleosides with the nerve cells. Both protein and RNA syntheses with ‘intact’ cells were sensitive to hypoxic or glucose-deficient conditions. While both the nerve and glial cells were sensitive to hypoxia to a similar extent, the nerve cells were more sensitive to glucose deficiency. It was suggested that the bulk isolated nerve and glial cells still retain certain integral cell functions as viable cells, and can be utilized for various physiological and pharmacological investigations provided caution is exercised in their application and in the interpretation of the results. 相似文献
12.
DNA sequence polymorphisms in transthyretin (TTR) genes were investigated by single-strand conformation polymorphism (SSCP) analysis of polymerase chain reaction products. The amplified DNA fragments that encode each exon of the normal TTR gene showed two bands, representing the two complementary single strands of DNA. In one patient with amyloid polyneuropathy, the exon 3 DNA showed a unique, aberrant migration pattern. Direct sequencing analysis of the amplified exon 3 revealed a single base change (G-to-T), resulting in a novel amino acid substitution (Ser-50----Ile). We also present the SSCP patterns for five known Japanese TTR variants. 相似文献
13.
Mitsuhide Hamaguchi Hironari Kamikubo Kayo N. Suzuki Yoshihisa Hagihara Itaru Yanagihara Ikuhiro Sakata Mikio Kataoka Daizo Hamada 《PloS one》2013,8(8)
Enterohaemorrhagic E. coli (EHEC) induces actin reorganization of host cells by injecting various effectors into host cytosol through type III secretion systems. EspB is the natively partially folded EHEC effector which binds to host α-catenin to promote the actin bundling. However, its structural basis is poorly understood. Here, we characterize the overall structural properties of EspB based on low-resolution structural data in conjunction with protein dissection strategy. EspB showed a unique thermal response involving cold denaturation in the presence of denaturant according to far-UV circular dichroism (CD). Small angle X-ray scattering revealed the formation of a highly extended structure of EspB comparable to the ideal random coil. Various disorder predictions as well as CD spectra of EspB fragments identified the presence of α-helical structures around G41 to Q70. The fragment corresponding to this region indicated the thermal response similar to EspB. Moreover, this fragment showed a high affinity to C-terminal vinculin homology domain of α-catenin. The results clarified the importance of preformed α-helix of EspB for recognition of α-catenin. 相似文献
14.
Hitoshi Ando Hayato Yanagihara Koh‐ichi Sugimoto Yohei Hayashi Shuichi Tsuruoka Toshinari Takamura 《Chronobiology international》2013,30(4):655-665
Recent studies have shown the gene expression of several transporters to be circadian rhythmic. However, it remains to be elucidated whether the expression of P‐glycoprotein, which is involved in the transport of many medications, undergoes 24 h rhythmicity. To address this issue, we investigated daily profiles of P‐glycoprotein mRNA and protein levels in peripheral mouse tissues. In the liver and intestine, but not in the kidney, Abcb1a mRNA expression showed clear 24 h rhythmicity. On the other hand, Abcb1b and Abcb4, the other P‐glycoprotein genes, did not exhibit significant rhythmic expression in the studied tissues. In the intestine, levels of whole P‐glycoprotein also exhibited a daily rhythm, with a peak occurring in the latter half of the light phase and a trough at the onset of the light phase. Consistent with the day‐night change of P‐glycoprotein level, the ex vivo accumulation of digoxin, an Abcb1a P‐glycoprotein substrate, into the intestinal segments at the onset of dark phase was significantly lower than it was at the onset of the light phase. Thus, Abcb1a P‐glycoprotein expression, and apparently its function, are 24 h rhythmic at least in mouse intestine tissue. This circadian variation might be involved in various chronopharmacological phenomena. 相似文献
15.
Shinya Harusawa Koichi Sawada Takuji Magata Hiroki Yoneyama Lisa Araki Yoshihide Usami Kouta Hatano Kouichi Yamamoto Daisuke Yamamoto Atsushi Yamatodani 《Bioorganic & medicinal chemistry letters》2013,23(23):6415-6420
S-Alkyl-N-alkylisothiourea compounds containing various cyclic amines were synthesized in the search for novel nonimidazole histamine H3 receptor (H3R) antagonists. Among them, four N-alkyl S-[3-(piperidin-1-yl)propyl]isothioureas 18, 19, 22, and 23 were found to exhibit potent and selective H3R antagonistic activities against in vitro human H3R, but were inactive against in vitro human H4R. Furthermore, three alkyl homologs 18–20 showed inactivity for histamine release in in vivo rat brain microdialysis, suggesting differences in antagonist affinities between species. In addition, in silico docking studies of N-[4-(4-chlorophenyl)butyl]-S-[3-piperidin-1-yl)propyl]isothiourea 19 and a shorter homolog 17 with human/rat H3Rs revealed that structural differences between the antagonist-docking cavities of rat and human H3Rs were likely caused by the Ala122/Val122 mutation. 相似文献
16.
Kouta Takeda Takuya Ishida Kiyohiko Igarashi Masahiro Samejima Hiroyuki Ohno 《Bioscience, biotechnology, and biochemistry》2013,77(7):1195-1198
Pyrroloquinoline quinone-dependent quinoprotein alcohol dehydrogenases (PQQ-ADH) require ammonia or primary amines as activators in in vitro assays with artificial electron acceptors. We found that PQQ-ADH from Pseudomonas putida KT2440 (PpADH) was activated by various primary amines, di-methylamine, and tri-methylamine. The alcohol oxidation activity of PpADH was strongly enhanced and the affinity for substrates was also improved by pentylamine as an activator. 相似文献
17.
Junichi Satoh Hiroyuki Koshino Kouta Sekino Shinsaku Ito Ryo Katsuta Kouji Takeda 《Bioscience, biotechnology, and biochemistry》2016,80(2):363-367
A new compound in cucumber, Cucumis sativus, nutrient solution that appears under iron-deficient conditions, but not under ordinary culture conditions, has been revealed by HPLC analysis. The chemical structure of this compound was identified using LC-MS and NMR techniques as that of 4′-ketoriboflavin. This is the first report to show that 4′-ketoriboflavin can be found in metabolites from organisms. 相似文献
18.
19.
20.
Yagita Y Kitagawa K Taguchi A Ohtsuki T Kuwabara K Mabuchi T Matsumoto M Yanagihara T Hori M 《Journal of neurochemistry》1999,72(4):1544-1551