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911.
Haruhiko Yokoi Shingo Mori Jun Hirose Sachio Hayashi Yoshiyuki Takasaki 《Biotechnology letters》1998,20(9):895-899
A photosynthetic bacterium having ability to produce H2 from acetic, butyric and lactic acids, Rhodobacter sp. M-19 was isolated. H2 was produced from starch in a batch culture by Clostridium butyricum and in a two-step batch culture by C. butyricum and Rhodobacter sp. M-19 in yields of 1.9 and 3.6 mol H2/mol glucose, respectively. A mixed culture of C. butyricum and Rhodobacter sp. M-19 produced H2 from starch with a yield of 6.6 mol H2/mol glucose in a fed-batch culture. © Rapid Science Ltd. 1998 相似文献
912.
913.
H2 production from starch by a mixed culture of Clostridium butyricum and Enterobacter aerogenes 总被引:6,自引:0,他引:6
Haruhiko Yokoi Tadafumi Tokushige Jun Hirose Sachio Hayashi Yoshiyuki Takasaki 《Biotechnology letters》1998,20(2):143-147
A mixed continuous culture of Clostridium butyricum and Enterobacter aerogenes removed O2 in a reactor and produced H2 from starch with yield of more than 2 mol H2/mol glucose without any reducing agents in the medium. Co-immobilized cells of the bacteria on porous glass beads evolved H2 from starch at 1.3 l/l.h, with H2 yield of 2.6 mol H2/ mol glucose at dilution rate of 1.0 h–1 in a continuous culture. 相似文献
914.
We developed a novel flow particle analyzer that automatically classifies airborne pollen grains. The design of the particle
counter (model KP-1000) is based on that of a flow cytometer, applied to the measurement of airborne particles. The counter
classifies pollen species by simultaneously detecting both scattered light and the characteristic fluorescence excited by
ultraviolet light in the flow cell. We observed airborne pollen using KP-1000 pollen counters and Durham samplers to compare
their performance at three study sites in Japan during the spring pollen season. The pollen counter followed the variation
in pollen concentrations, and its daily pollen counts were significantly correlated with the results of the Durham sampling
method at all study sites. Although the counter over- or under-counted 2 target pollen species (Cryptomeria japonica and Chamaecyparis obtusa) when they coexisted, a data correction based on the Durham sampling results improved the accuracy of pollen classification
of the counter. Our results indicate that the new pollen counter has a strong potential for counting and identifying airborne
pollen grains in real time, and it requires further improvement, field trials, and tests with other common airborne pollen
grains. 相似文献
915.
Autoantibodies recognizing proteins copurified with PCNA in patients with connective tissue diseases
Ken Takeuchi Kazuhiko Kaneda Ikuko Kawakami Yoshinari Takasaki Hiroshi Hashimoto 《Molecular biology reports》1996,23(3-4):243-246
Objective. Proliferating cell nuclear antigen (PCNA), one of the target antigen recognized by lupus sera, has been reported to be present as a subnuclear multi-peptide complex. But autoantibodies reacting with components of PCNA complex are poorly understood. To study the specificity of those autoantibodies, immunoreactivities of autoimmune sera against purified PCNA antigen were studied. Methods. PCNA antigens were purified from rabbit thymus extract by affinity column using murine monoclonal antibodies (mAbs) to PCNA, TOB7, TO17 and TO30. Immunoreactivities of autoimmune sera against purified PCNA were analyzed by WB. Results. PCNA antigen purified by serum AK predominantly showed a 34 kD band specific for PCNA in SDS-PAGE. When antigens were purified by anti-PCNA mAb TOB7 and TO30 which are known to be targeting different epitopes on PCNA antigen, SDS-PAGE analysis showed various mol. wt of proteins in addition to the 34 kD PCNA while both AK and mAbs reacted only with 34 kD PCNA in WB. In WB using PCNA purified by TOB7, various immunoreactivities were observed at 150, 66, 58, 48, 45, 37, 32 and 16 kDa in sera from patients with connective tissue diseases. Conclusions. These results suggested that many of the proteins copurified with PCNA were also targets of autoimmune responses and these autoantibody experssion may be induced through antigen-driven mechanisms.Abbreviations mAb
monoclonal antibody
- PCNA
proliferating cell nuclear antigen
- PCNA/AK
PCNA affinity purified by antibodies from patient serum AK
- PCNA/TO30
PCNA purfied by mAb TO30
- PCNA/TOB7
PCNA purified by mAb TOB7
- SLE
systemic lupus erythematosus 相似文献
916.
Kotaro T. Yamamoto 《Journal of plant research》1990,103(4):469-491
Phytochrome is a proteinaceous pigment that acts as a photoreceptor for photomorphogenetic responses in plants. It exists
as two stable absorbing forms, Pr and Pfr, which are interconvertible reversibly by irradiation with red or far-red light. The present review discusses (i) the primary
and higher-order structures of phytochrome that permit the reversible photoreaction; (ii) the molecular properties which change
accompanying the phototransformation; and (iii) the four-leaved shape model which has recently been proposed as a model of
quaternary structure of phytochrome. 相似文献