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We have examined whether heavy meromyosin (HMM) consists of a single kind of active site by analyzing the changes in the relative MgATPase activity and the relative amplitude of the ATP-induced fluorescence enhancement of the protein when the fraction of HMM "affinity"-labeled by vanadate plus ADP was varied. The analysis is based on a prediction that these two changes should be proportional to each other if myosin consists of a single kind of active site and generates the rate-limiting myosin**product complex emitting enhanced fluorescence. Although the difference between these two changes was very small with native HMM, it was large with HMM in which 5 fast-reactive sulfhydryl-groups per head were pre-modified with thimerosal. The difference indicated the existence of heterogeneous active sites in the SH-modified HMM. The results were best explained in terms of the hypothesis that fifty percent of the active site splits MgATP by a mechanism giving a fluorescence enhancement whereas the other fifty percent splits MgATP by another mechanism giving no fluorescence enhancement. Two possible explanations for the existence of heterogeneous active sites in the SH-modified HMM are discussed. One assumes the pre-existence of some sort of 1:1 heterogeneity in the micro-environment of the active sites and the other, which is considered less likely, assumes the introduction of the heterogeneity as a result of the SH-modification. 相似文献
997.
Transformation of arachidonic acid into thromboxane B2 by the homogenates of activated macrophages 总被引:4,自引:0,他引:4
The homogenates of activated macrophages obtained from liquid paraffin-injected guinea pig peritoneum were incubated with [14C]arachidonic acid or with radioactive prostaglandin endoperoside [14C]prostaglandin H2. The major radioactive metabolite in both cases was thromboxane B2, which was identified by NaBH4 reduction, rechromatography and autoradiography. 相似文献
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9 halogenated alkanols, 9 corresponding tris (haloalkyl)phosphates, and 2 bis-(2,3-dibromopropyl)phosphate salts were evaluated for mutagenicity against Salmonella typhimurium TA98, TA100, TA1535, TA1537 and TA1538, with and without rat liver in vitro metabolic activation system (S9 mix). Most of the test samples showed mutagenic activity in the strains TA100 and TA1535, but not in the strains TA98, TA1537 and TA1538. In general, the mutagenic activities of the phosphates obtained with S9 mix were greater than the activities obtained without S9 mix. Among the phosphates, several structure--activity relationships were found; i.e., (i) the bromoalkyl derivatives were more mutagenic than the corresponding chloroalkyl derivatives, (ii) the beta-haloethyl derivatives were more mutagenic than the gamma-halopropyl derivatives, (iii) the phosphates having adjacent beta and gamma halogen atoms in the alkyl moiety, e.g., tris-(2,3-dibromopropyl)phosphate, were particularly potent mutagens, (iv) the branched carbon chain reduced the mutagenic activities in spite of the presence of beta-halogen atoms, e.g., tris(1-bromomethyl-2-bromoethyl)phosphate. However, such relations did not necessarily apply to the halogenated alkanols. It is concluded that the metabolic activation pathway via haloalkanols to mutagens must not be in common with all tris-BP-like phosphates. 相似文献
1000.
A Kitamura Y Tanigawa T Yamamoto M Kawamura S Doi M Shimoyama 《Biochemical and biophysical research communications》1979,87(3):725-733
The effect of glucocorticoid hormone administration on the nuclear poly(ADP-ribose) synthetase activity of chick embryoliver was investigated. Compared with the values obtained with control nuclei, the enzyme activity was markedly reduced in the nuclei of liver prepared from chick embryo treated with 0.1 mg hydrocortisone for 12 hours or longer. The possible relationship between the reduction of poly(ADP-ribose) synthetase activity and decrease in DNA synthesis is discussed. 相似文献