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21.
Human brain cDNA libraries were screened with cDNA inserts corresponding to the mRNA for the Na+,K(+)-ATPase alpha-subunit from pig kidney. The results obtained demonstrate the existence of two highly homologous mRNAs encoding the alpha- and alpha III-isoforms of the Na+,K(+)-ATPase catalytic subunit. 相似文献
22.
O. A. Bezborodova I. V. Alekseenko E. R. Nemtsova A. A. Pankratov O. B. Filyukova R. I. Yakubovskaya M. B. Kostina V. K. Potapov E. D. Sverdlov 《Doklady. Biochemistry and biophysics》2018,483(1):326-328
In this study, we evaluated the antitumor activity of a gene therapy complex in which the tumor-specific control of the expression of the effector suicide gene FCU1 was performed using a two-vector system based on the site-specific Cre–LoxP recombinase system. The complex of interest showed a high therapeutic potential in a mouse colon adenocarcinoma model. 相似文献
23.
Floras of Oshin-Boro-Udzyur-Ula, Tsagan-Ovoo, and Nariin-Sukhait of Mongolia are reviewed. Their composition suggests a Middle Jurassic age for these floras. They resemble the synchronous floras of the West Siberian Province of the Siberian Paleofloristic Region. The boundary between this province and North Chinese Province of the same region should be drawn further to the south than was previously thought. The Middle Jurassic floras of Mongolia existed in a warm temperate, humid climate. 相似文献
24.
G. G. Chestukhina S. A. Tyurin L. I. Kostina A. L. Osterman I. A. Zalunin O. A. Khodova V. M. Stepanov 《Journal of Protein Chemistry》1990,9(4):501-507
N-Terminal domain (65 kD) of -endotoxin produced byBacillus thuringiensis ssp.alesti, as shown by limited proteolysis, consists of two subdomains of molecular mass 30 and 33 kD that correspond, respectively, to conservative and variable regions of the -endotoxin primary structure. Furthermore, proteolysis of these subdomains leads to their conversion into at least two fragments of molecular mass 10 kD stable to proteinase action. Such a pattern of molecular organization appears to be common for several structurally related -endotoxins that belong to thekurstaki group. Entomicidal protein produced by ssp.israelensis (70 kD), which differs strongly fromalesti and otherkurstaki group -endotoxins, retains a similar type of molecular organization and consists of two subdomains with molecular mass of 35 kD. Apparently, the characteristic pattern of the -endotoxins' molecular structure reflects separation of functions (e.g., host recognition and toxicityper se) between domains and subdomains of these proteins. 相似文献
25.
26.
G. G. Chestukhina S. A. Tyurin L. I. Kostina A. L. Osterman I. A. Zalunin O. A. Khodova V. M. Stepanov 《The protein journal》1990,9(4):501-507
N-Terminal domain (65 kD) of δ-endotoxin produced byBacillus thuringiensis ssp.alesti, as shown by limited proteolysis, consists of two subdomains of molecular mass 30 and 33 kD that correspond, respectively, to conservative and variable regions of the δ-endotoxin primary structure. Furthermore, proteolysis of these subdomains leads to their conversion into at least two fragments of molecular mass 10 kD stable to proteinase action. Such a pattern of molecular organization appears to be common for several structurally related δ-endotoxins that belong to thekurstaki group. Entomicidal protein produced by ssp.israelensis (70 kD), which differs strongly fromalesti and otherkurstaki group δ-endotoxins, retains a similar type of molecular organization and consists of two subdomains with molecular mass of ~35 kD. Apparently, the characteristic pattern of the δ-endotoxins' molecular structure reflects separation of functions (e.g., host recognition and toxicityper se) between domains and subdomains of these proteins. 相似文献
27.
28.
V. A. Ryabinin E. V. Kostina A. N. Sinyakov 《Russian Journal of Bioorganic Chemistry》2013,39(3):338-340
A version of the universal oligonucleotide hybridization microchip with the size of 6 × 5 spots (4 × 4 mm) has been proposed, which operates on the principle of “one spot-one subtype.” This microchip may be the prototype of a biosensor for fixation of influenza A virus and typing of 15 subtypes of hemagglutinin and 9 subtypes of neuraminidase. 相似文献
29.
V. A. Ryabinin E. V. Kostina A. A. Neverov G. A. Maksakova A. N. Sinyakov 《Russian Journal of Bioorganic Chemistry》2010,36(5):634-644
A microarray for the subtyping of influenza A neuraminidase is presented. The selection of oligoprobes proceeded in two steps.
The first step included the selection of peptides specific for each subtype of neuraminidase. At the second step, the oligoprobes
were calculated using the found peptide structures with the subsequent additional selection of the most specific and representative
probes. From 19 to 24 probes were used for the determination of each neuraminidase subtype. The microarray testing for 19
samples with the most widespread types (N1 and N2) specifies in an unequivocal definition 18 of them and only 1 isolate has
not been identified. 相似文献
30.
G S Ovchinnikov YuAMonastyrskaya N E Broude A M Ushkaryov YuAMelkov I V Smirnov YuVMalyshev R L Allikmets M B Kostina I E Dulubova 《FEBS letters》1988,233(1):87-94
The primary structure of a gene of the Na+, K+-ATPase multigenic family in the human genome has been determined. The gene corresponds to a hypothetical alpha III-form of the enzyme catalytic subunit. The gene comprises over 25,000 bp, and its protein coding region includes 23 exons and 22 introns. Possible correlation between structural features of the protein and location of introns in the gene are discussed. 相似文献