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11.
Briones Carlos Koroutchev Kostadin Amils Ricardo 《Origins of life and evolution of the biosphere》1998,28(4-6):571-582
In order to study the functional phylogeny of organisms, forty different protein synthesis inhibitors with diverse domain and funcional specificities have been used to analyze forty archaeal, bacterial and eukaryotic translational systems. The inhibition curves generated with the different ribosome-antibiotic pairs have shown very interesting similarities among organisms belonging to the same phylogenetic group, confirming the feasibility of using such information in the development of evolutionary studies. A new method to extract most of the information contained in the inhibition curves is presented. Using a statistical treatment based on the principal components analysis of the data, we have defined coordinates for the organisms which have allowed us to perform a functional clustering of them. The phenograms obtained are very similar to those generated by 16/18S rRNA sequence comparison. These results prove the phylogenetic value of our functional analysis and suggest an interesting intersection between genotypic and phenotypic (functional) information. 相似文献
12.
Nicolas Friess Jrg C. Müller Pablo Aramendi Claus Bssler Martin Brndle Christophe Bouget Antoine Brin Heinz Bussler Kostadin B. Georgiev Radosaw Gil Martin M. Gossner Jacob Heilmann‐Clausen Gunnar Isacsson Anton Kritín Thibault Lachat Laurent Larrieu Elodie Magnanou Alexander Maringer Ulrich Mergner Martin Mikol Lars Opgenoorth Jürgen Schmidl Miroslav Svoboda Simon Thorn Kris Vandekerkhove Al Vrezec Thomas Wagner Maria‐Barbara Winter Livia Zapponi Roland Brandl Sebastian Seibold 《Diversity & distributions》2019,25(5):783-796
13.
The amplification of the TLO (for telomere-associated) genes in Candida albicans, compared to its less pathogenic, close relative Candida dubliniensis, suggests a role in virulence. Little, however, is known about the function of the Tlo proteins. We have purified the Mediator coactivator complex from C. albicans (caMediator) and found that Tlo proteins are a stoichiometric component of caMediator. Many members of the Tlo family are expressed, and each is a unique member of caMediator. Protein expression analysis of individual Tlo proteins, as well as the purification of tagged Tlo proteins, demonstrate that there is a large free population of Tlo proteins in addition to the Mediator-associated population. Coexpression and copurification of Tloα12 and caMed3 in Escherichia coli established a direct physical interaction between the two proteins. We have also made a C. albicans med3Δ/Δ strain and purified an intact Mediator from this strain. The analysis of the composition of the med3Δ Mediator shows that it lacks a Tlo subunit. Regarding Mediator function, the med3Δ/Δ strain serves as a substitute for the difficult-to-make tloΔ/Δ C. albicans strain. A potential role of the TLO and MED3 genes in virulence is supported by the inability of the med3Δ/Δ strain to form normal germ tubes. This study of caMediator structure provides initial clues to the mechanism of action of the Tlo genes and a platform for further mechanistic studies of caMediator's involvement in gene regulatory patterns that underlie pathogenesis. 相似文献