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E. N. Kosobokova K. A. Skrypnik M. V. Pinyugina A. I. Shcherbakov V. S. Kosorukov 《Applied Biochemistry and Microbiology》2014,50(8):773-779
A method for the production and purification of biologically active recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF), expressed in Escherichia coli, has been developed. Washing of an inclusion body fraction which allows the removal of numerous ballast proteins and on-column refolding of rhGM-CSF are specific characteristics of the method. The refolding efficiency reached 70%; the purity of the preparation constituted 95%. The biological activity was similar to those of other recombinant hGM-CSF analogs. 相似文献