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21.
R Alan Harris Dorottya Nagy-Szakal Sabina AV Mir Eibe Frank Reka Szigeti Jess L Kaplan Jiri Bronsky Antone Opekun George D Ferry Harland Winter Richard Kellermayer 《Epigenetics》2014,9(8):1131-1137
Inflammatory bowel diseases (IBD) are emerging globally, indicating that environmental factors may be important in their pathogenesis. Colonic mucosal epigenetic changes, such as DNA methylation, can occur in response to the environment and have been implicated in IBD pathology. However, mucosal DNA methylation has not been examined in treatment-naïve patients. We studied DNA methylation in untreated, left sided colonic biopsy specimens using the Infinium HumanMethylation450 BeadChip array. We analyzed 22 control (C) patients, 15 untreated Crohn’s disease (CD) patients, and 9 untreated ulcerative colitis (UC) patients from two cohorts. Samples obtained at the time of clinical remission from two of the treatment-naïve UC patients were also included into the analysis. UC-specific gene expression was interrogated in a subset of adjacent samples (5 C and 5 UC) using the Affymetrix GeneChip PrimeView Human Gene Expression Arrays. Only treatment-naïve UC separated from control. One-hundred-and-twenty genes with significant expression change in UC (> 2-fold, P < 0.05) were associated with differentially methylated regions (DMRs). Epigenetically associated gene expression changes (including gene expression changes in the IFITM1, ITGB2, S100A9, SLPI, SAA1, and STAT3 genes) were linked to colonic mucosal immune and defense responses. These findings underscore the relationship between epigenetic changes and inflammation in pediatric treatment-naïve UC and may have potential etiologic, diagnostic, and therapeutic relevance for IBD. 相似文献
22.
Vodopyanov S. O. Oleinikov I. P. Goncharov E. K. Duvanova O. V. Mishan'kin M. B. Suchkov I. Yu. Vodopyanov A. S. Shishiyanu M. V. Shcherbakova E. V. Kornienko I. V. Mishan'kin B. N. 《Molecular Biology》2002,36(6):874-878
Computer analysis revealed seven potential variable-number tandem-repeat (VNTR) loci in the Vibrio cholerae genome. Specific primers were designed to amplify locus VcA located on chromosome 2 and containing a TGCTGT repeat. The locus was found in all tested strains from aV. cholerae strain collection, the repeat number varying from 3 to 23. In total, 14 VcA alleles were observed. The VcA locus was proposed as a marker for the molecular typing of V. cholerae strains. 相似文献
23.
The entire cycle of larval development of the spider crab Pugettia quadridens (de Haan, 1850) (Decapoda: Majidae), widespread in Peter the Great Bay (Sea of Japan) is studied under the laboratory conditions. The development cycle of this species comprises prezoea, zoea I, zoea II, and megalopa. At a temperature of 18–20° C larval development took from 11 to 15 days. Zoea II is described in detail for the first time. Many morphological characters are found distinguishing zoea and megalopa of P. Quadridens in Russian waters from the larvae of this species in Japanese and Korean waters. Some characters of larvae are similar in P. Quadridens and the related species of the genus Pugettia. The larvae of P. Quadridens occur in the plankton of Vostok Bay from late June to late October with a density up to 5 ind/m3 at a surface water temperature of 13–21°C. They are easily distinguished from the other brachyuran larvae of this region by the absence of lateral spines on the carapace.Original Russian Text Copyright © 2004 by Biologiya Morya, Kornienko, Korn. 相似文献
24.
Sinichkin AA Maslova MN Medvedeva IA Kornienko IE 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》1998,84(11):1247-1251
Immobilisation stress (IMS) led to a 42% decrease in erythrocyte Na, K-ATPase activity in rats. Pre-treatment of the "stressed" erythrocytes with human serum albumin (HSA) and 1-day exposition of the HSA prior to the IMS led to stabilising of enzyme activity at the control level. Absence of inhibiting effect of non-protein supernatants of the blood plasma of stressed rats on enzyme activity of normal erythrocytes was shown in presence of the HSA both in vitro and in vivo. The mechanism of the HSA protective effect on the Na,K-ATPase activity of erythrocytes in the IMS, is discussed. 相似文献
25.
26.
AV Shevchenko IG Budzanivska TP Shevchenko VP Polischuk D Spaar 《Archives Of Phytopathology And Plant Protection》2013,46(2):139-146
The work was focused on the investigation of possible dependencies between the development of viral infection in plants and the presence of high heavy metal concentrations in soil. Field experiments have been conducted in order to study the development of systemic tobacco mosaic virus (TMV) infection in Lycopersicon esculentum L. cv. Miliana plants under effect of separate salts of heavy metals Cu, Zn and Pb deposited in soil. As it is shown, simultaneous effect of viral infection and heavy metals in tenfold maximum permissible concentration leads to decrease of total chlorophyll content in experiment plants mainly due to the degradation of chlorophyll a. The reduction of chlorophyll concentration under the combined influence of both stress factors was more serious comparing to the separate effect of every single factor. Plants' treatment with toxic concentrations of lead and zinc leaded to slight delay in the development of systemic TMV infection together with more than twofold increase of virus content in plants that may be an evidence of synergism between these heavy metal's and virus' effects. Contrary, copper although decreased total chlorophyll content but showed protective properties and significantly reduced amount of virus in plants. 相似文献
27.
Mamedov FR Arutiunov NV Usachev DIu Mel'nikova-Pitskhelauri TV Piashina DV Fadeeva LM Kornienko VN 《Vestnik rentgenologii i radiologii》2011,(1):4-10
The diagnostic arsenal for extracranial stenosis includes both ultrasound techniques, such as transcranial Doppler and duplex sonography, which are not only screening methods, but also are used at surgery, and high-tech neuroradiological techniques, such as spiral computed tomographic and magnetic resonance angiography, which can visualize structural changes in the vessel walls and qualitatively and functionally evaluate blood flow in the vascular bed. The purpose of this investigation was to determine the efficacy of non-invasive diagnostic methods, such as magnetic resonance angiography, including phase-contrast magnetic resonance angiography and spiral computed tomographic angiography, in the detection and comprehensive evaluation of stenoses and to compare the feasibilities of noninvasive procedures with the gold standard--selective angiography. 相似文献
28.
Anticancer evaluation of structurally diverse Amaryllidaceae alkaloids and their synthetic derivatives 总被引:1,自引:0,他引:1
Plants of the Amaryllidaceae family have been under intense scrutiny for the presence of the specific metabolites responsible
for the medicinal properties associated with them. The study began in 1877 with the isolation of alkaloid lycorine from Narcissus pseudonarcissus and since then more than 100 alkaloids, exhibiting diverse biological activities, have been isolated from the Amaryllidaceae
plants. Based on the present scientific evidence, it is likely that isocarbostyril constituents of the Amaryllidaceae, such
as narciclasine, pancratistatin and their congeners, are the most important metabolites responsible for the therapeutic benefits
of these plant species in the folk medical treatment of cancer. Notably, Narcissus poeticus L., used by the ancient Greek physicians, is now known to contain about 0.12 g of narciclasine per kg of fresh bulbs. The
focus of the present research work is the chemistry and biology of these compounds as specifically relevant to their potential
use in medicine. In particular, the anticancer evaluation of lycorine, narciclasine as well as of other Amaryllidaceae alkaloids
and their synthetic derivatives are presented in this paper. The structure–activity relationships among some groups of Amaryllidaceae
alkaloids will be discussed. 相似文献
29.
In elite runners, the ventilation influx, ventilation debt, and ventilation demand of the exercises were calculated on the basis of the pulmonary respiration dynamics during the maximum workout and recovery. The breathing values proved to closely reproduce the changes in the main parameters of oxygen demand at high intensity and duration of the exercise and can be used for quantification and standardization of exercise loads in sports. Three important factors of the aerobic exchange in the body were found to ensure the high level of the sports achievements in running: (1) general increase in the level of pulmonary ventilation (VE), oxygen demand (VO2), and release of carbon dioxide (CO2); (2) intensity of oxygen supply from lungs to the working muscles; (3) the rate of oxygenation (StO2) and total rate of blood circulation. 相似文献
30.
Sixteen girls were observed for five years during the age period from 7 to 11. The longitudinal study included the annual
assessment of constitutional characteristics according to Shtefko-Ostrovskii and the calculation of endomorphy, mesomorphy,
and ectomorphy indices from anthropometric data according to Heath-Carter. The indices of skeletal muscle power (aerobic capacity
and power; anaerobic power, and power indexa) were determined in a two-load ergometric test using Muller’s equation. The girls demonstrated a 2.5-time more stable somatotype
than boys. The most pronounced changes in their constitutional characteristics were observed between the ages of 8 and 9,
i.e., a year earlier than in boys. These changes occurred only within the dolichomorphic and brachymorphic groups without
transitions between somatotypes. In girls of all ages, the constitutional characteristics were in good correlation with skeletal
muscle power. In general, dolichomorphs were characterized by high aerobic indices: their mean aerobic capacity was as high
as 83.1 kJ/kg vs. 4.1 kJ/kg in brachymorphs, who showed preferential development of anaerobic mechanisms. 相似文献