从睡眠剥夺树鼩（TBC）尿液提取内源性“睡眠因子”（sleep factor）的研究

本研究报道从睡眠剥夺（ＳＤ）４８—７２ｈ的灵长类原宗（ｐｒｉｍｉｔｉｖｅｓｔｏｃｋ）Ｔｕｐａｉａｂｅｌａｎｇｅｒｉｃｈｉｎｅｎｓｉｓ（ＴＢＣ）提取内源性“睡眠因子”（ｓｌｅｅｐｆａｃｔｏｒ）Ｓ２Ｃ和Ｓ４Ｂ。收集的尿液经超滤,清液冻干经ＳｅｐｈａｄｅｘＧ１５分离得到ＦｒａｃｔｉｏｎⅠ－Ⅴ。活性测定发现Ｆｒａｃｔｉｏｎ－Ⅲ（Ｓ２Ｃ）呈现显著δ－增强促眠效应。经ＳｅｐｈａｄｅｘＧ２５和ＳｅｐｈａｄｅｘＬＨ２０进一步净化的Ｓ４Ｂ也呈现显著δ－增强促眠效应。     

P物质对GABAA和GABAB受体介导的DRG神经元膜反应的调制作用

实验在幼年大鼠ＤＲＧ标本上进行。应用细胞内记录观察了ＳＰ对ＧＡＢＡ反应的调制作用。结果证明：（１）单独滴加ＳＰ（５×１０（－６）－４×１０（－５）ｍｏｌ／Ｌ）或浴槽灌流ＳＰ（１０（－６）－５×１０（－６）ｍｏｌ／Ｌ）不引起膜电位的改变或仅有轻微的去极化,但却能使ＧＡＢＡ引起的去极化反应减小５０．８±２０．２％（±ＳＤ）（２０／３０）;（２）单独滴加ＳＰ可使多数受检细胞ＡＰＤ５０延长２８．７±９．１％（±ＳＤ）（１０／１８）;（３）在预加ＳＰ后,能使ｂａｃｌｏｆｅｎ所引起的ＡＰＤ５０缩短效应（２０．６±２．９％,±ＳＤ）完全消除（４／１２）或翻转成ＡＰＤ（５０）延长１９．３±８．９％（±ＳＤ）（８／１２）;（４）预加ＧＡＢＡＢ受体激动剂ｂａｃｌｏｆｅｎ（１０（－４）－１０（－３）ｍｏｌ／Ｌ）３０—９０ｓ后明显地抑制ｍｕｓｃｉｍｏｌ（１０－４－１０－３ｍｏｌ／Ｌ）引起的去极化反应,其抑制效应达５４．４±１８．８％（±ＳＤ）（１７／２０）。由于ＤＲＧ神经元的胞体通常可用来作为研究初级传入终末的模型,因而本文实验结果提示：介导伤害性刺激信息的Ｐ物质在背角的释放,可能作用于初级传入终末,从而产生对抗ＧＡＢＡ介导的突触     

纵坑切梢小蠹蛀梢期空间分布

在昆明地区,纵坑切梢小蠹(Tomicus piniperda)成虫蛀梢多集中在蛀干木附近。 种群密度以蛀干木为中心向周围呈指数递减,散布半径约30m。在蛀梢过程中,该种群逐渐向新区扩张。在树冠内,纵坑切梢小蠹主要分布在4-10轮枝上。第7轮枝虫口百分率最高。6-7轮枝受害率最大。 树冠上层受害较其下层严重。从树冠水平层次考察,树冠外层虫量相对集中,约为树冠中、内层虫量之和。 树冠内层虫量最少。纵坑切梢小蠹在树冠内的种群分布系由梢径、种群密度、蛀梢行为、降落方式、光照等因素综合影响的结果。     

马尾松毛虫的光周期反应

马尾松毛虫Dendrolimus punctatus Walker和油松毛虫 Dendrolimus tabulaeformisTsai et Liu都属于长日照型昆虫。它们的光周期反应却存在着明显的差开。马尾松毛虫初龄幼虫对光照时数虽然敏感,可是只有在整个幼虫期都处在短光照周期条件下才能维持幼虫的滞育。 而且, 幼虫的滞育强度明显较弱,解除滞育所要求的条件也不如油松毛虫严格。在较高温度条件下,幼虫的滞育更不稳定,一般仅表现为生长的短暂停滞和幼虫历期的相对延长。温度和光周期还可影响到幼虫的存活和性比。马尾松毛虫幼虫呈典型的浅滞育现象。正是由于这一特性,在环境条件的影响下,长江沿岸省份不同年份马尾松毛虫二、三代分化的比例才有较大的波动,这直接关系着当地的种群动态,也是当年当地高虫口区二代虫能否成灾的关键。     

Chaetomella acutiseta produces chaetomellic acids A and B which are reversible inhibitors of farnesyl-protien transferase

Chaetomellic acids A and B, isolated from Chaetomella acutiseta, are specific inhibitors of farnesyl-protein transferase that do not inhibit geranylgeranyl transferase type 1 or squalene synthase. Chaetomellic acids A and B are reversible inhibitors, resemble farnesyl diphosphate and probably inhibit FPTase by substituting for farnesyl diphosphate. Chaetomellic acid production appears to be widespread within the genus Chaetomella. Correspondence to: R. B. Lingham     

Influence of submergence on growth of seedlings of Scirpus lacustris and Phragmites australis

• 1 Seeds of Scirpus lacustris and Phragmites australis were germinated in early June, and twenty-four seedlings of each species were subsequently exposed to submerged conditions (eight seedlings at each of the water depths 0.2, 0.4 and 0.8m), in outdoor 500–1 tanks in southern Sweden. Weight and shoot length of the plants were measured in September.
• 2 The Phragmites seedlings did not show any significant growth when submerged. The Scirpus seedlings, however, developed submerged leaves and exhibited considerable submerged growth. One Scirpus plant, in shallow water (0.2m), had developed an aerial shoot by September. Shoot length of the remaining (submerged) Scirpus plants was positively related to plant weight within water depth treatments, and was higher, in relation to plant weight, in deeper water. Mean weight in September of the submerged Scirpus plants decreased with increased water depth.
• 3 In south Swedish lakes with a lowered water table, Scirpus often occupies large areas on the lakeward side of the reed belt, which is generally dominated by Phragmites. The differences between the two species, in performance of submerged seedlings, suggest that this zonation may be created through successful submerged seedling establishment of Scirpus on the lakeward side of Phragmites.

     

牛生长激素释放因子的融合表达及其产物的化学加工

通过寡核苷酸引导的定位突变,在人工全合成的第２７位为Ｉｌｅ的牛生长激素释放因子［Ｉｌｅ２７］ｂＧＲＦ（１－４４）ＯＨ基因的５＇端ＡＴＧ后插入Ｔｒｐ密码子序列,并分别了构建了Ｐｌ ｐｒｏｍｏｔｅｒ控制下、以β－半乳糖苷酶和ｐｒｏｔｅｉｎ Ａ结合ＩｇＧ ｄｏｍａｉｎＢ、Ｃ为载体蛋白的融合型基因表达质粒ｐＢＬＥ３１０和ｐＢＬＰＡＥ２Ｄ,在大肠杆菌中得到高效表达。经ＳＤＳ－ＰＡＧＥ分析,表达产物β－Ｇａｌ     

假菠萝麻蛋白酶分离纯化及特性研究

从假菠萝麻叶汁中用乙醇分部沉淀法得到一种蛋白酶,对酪蛋白有强烈的水解活性,经Ｓｅｐｈａｄｅｘ Ｇ－１００柱层析和ＳＰ－Ｓｅｐｈａｄｅｘ Ｃ－５０离子交换柱层析等步骤纯化,可得到六角形结晶。结晶酶液经ＰＡＧＥ圆盘电泳,每条胶柱上只显示一条蛋白带,其活性在ｐＨ４。５－１０、５５℃范围内较稳定,最适ｐＨ８．５最适温度５０℃,Ｋｍ（酪蛋白）值为０．１８５％（Ｗ／Ｖ）。用ＳＤＳ－ＰＡＧＥ和Ｓｅｐｈａｄｅｘ     

The inhibitory effect of transthyretin gene on growth of human hepatoma cells

Transthyretin(TTR) gene was highly expressed in normal liver and it has been found to be deleted in part of DNA samples from human hepatic cancer.Its mRNA expression was suppressed in most hepatoma samples.In order to study the biological effect of TTR gene on the growth of hepatoma cells,a recombinant vector containing TTR cDNA was constructed by pCMV,then it was transfected into hepatoma cell lines SMMC-7721 and Q3.It has been demonstrated that the inhibition of growth rate of TTR cDNA transfected hepatoma cells was about 50% in strength compared with that of the control.This inhibition was further enhanced when the transfected hepatoma cells were treated with all-trans retinoic acid.Hepatoma cells of cell lines PLC/PRF/5,SMMC-7721 and Q3 as well as hepatoma cells SMMC-7721 transfected with pCMV or pCMV-TTR were analyzed for TTR expression by Northern hybridization.The low level of TTR expression was found in both hepatoma cell lines and in SMMC-7721 cells transfected with pCMV alone.However,a remarkable TTR mRNA expression was observed in hepatoma SMMV-7721 cells transfected with pCMV-TTR.It seems possible that TTR gene might be a candidate of cancer suppressor gene for human hepatic cancer.     

急性脑出血综合治疗的几个问题探讨：附死亡80例分析

通过对８０例急性脑出血死亡组分析,发现死于脑疝的占６２．５％,脑疝与一种或二种以上合并症同存者占７７．５％,显著高于存活组（Ｐ＜０．０１）．它们互为因果,加速死亡。因此,应重视早期或超早期采用简易定向锥颅脑内血肿碎吸术吸除血肿,同时注意维持生命体征稳定,加强脱水降颅压,预防、控制合并症等综合治疗。且要全面分析,相互兼顾,正确处置。这是帮助机体渡过调控障碍难关,挽救生命的有效治疗措施。