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91.
Shen YH Song GX Liu YQ Sun W Zhou LJ Liu HL Yang R Sheng YH Qian LM Kong XQ 《Journal of bioenergetics and biomembranes》2012,44(3):317-323
Fatty acid binding protein 3 (FABP3) (also known as H-FABP) is a member of the intracellular lipid-binding protein family, and is mainly expressed in cardiac muscle tissue. The in vivo function of FABP3 is proposed to be in fatty acid metabolism, trafficking, and cell signaling. Our previous study found that FABP3 is highly regulated in patients with ventricular septal defect (VSD), and may play a significant role in the development of human VSD. In the present study, we aimed to investigate the impact of FABP3 knockdown by RNA interference (RNAi) on apoptosis and mitochondrial function of embryonic carcinoma (P19) cells. The results revealed that downregulated FABP3 expression promoted apoptosis, and resulted in mitochondrial deformation, increased mitochondrial membrane potential (MMP), and decreased intracellular ATP synthesis. In addition, the knockdown of FABP3 also led to excess intracellular ROS production. However, there was no obvious influence on the amount of mitochondrial DNA. Collectively, our results indicated that FABP3 knockdown promoted apoptosis and caused mitochondrial dysfunction in P19 cells, which might be responsible for the development of human VSD. 相似文献
92.
Association between the gene encoding 5-lipoxygenase-activating protein and stroke replicated in a Scottish population 总被引:6,自引:0,他引:6 下载免费PDF全文
Helgadottir A Gretarsdottir S St Clair D Manolescu A Cheung J Thorleifsson G Pasdar A Grant SF Whalley LJ Hakonarson H Thorsteinsdottir U Kong A Gulcher J Stefansson K MacLeod MJ 《American journal of human genetics》2005,76(3):505-509
Cardiovascular diseases, including myocardial infarction (MI) and stroke, most often occur on the background of atherosclerosis, a condition attributed to the interactions between multiple genetic and environmental risk factors. We recently reported a linkage and association study of MI and stroke that yielded a genetic variant, HapA, in the gene encoding 5-lipoxygenase-activating protein (ALOX5AP), that associates with both diseases in Iceland. We also described another ALOX5AP variant, HapB, that associates with MI in England. To further assess the contribution of the ALOX5AP variants to cardiovascular diseases in a population outside Iceland, we genotyped seven single-nucleotide polymorphisms that define both HapA and HapB from 450 patients with ischemic stroke and 710 controls from Aberdeenshire, Scotland. The Icelandic at-risk haplotype, HapA, had significantly greater frequency in Scottish patients than in controls. The carrier frequency in patients and controls was 33.4% and 26.4%, respectively, which resulted in a relative risk of 1.36, under the assumption of a multiplicative model (P=.007). We did not detect association between HapB and ischemic stroke in the Scottish cohort. However, we observed that HapB was overrepresented in male patients. This replication of haplotype association with stroke in a population outside Iceland further supports a role for ALOX5AP in cardiovascular diseases. 相似文献
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油菜细胞质雄性不育系叶绿体DNA特异片段的分子克隆 总被引:7,自引:0,他引:7
采用高离子浓度缓冲液法分别提取油菜不育系及保持系的叶绿体DNA。经Sepharcse 4B柱层析纯化后,得到具有较高纯度的叶绿体DNA样品。将其分别用Eco RI、Bam HI、HimdHI、PstI和XhoI 5种限制性内切酶酶解,得到5种限制性内切酶图谱。其中除PstI图谱外,其它4种酶谱均显示出明显的两系间叶绿体DNA结构差异。以pBR 322为载体,分别克隆了不育系Bam HI图谱上的3个特异片段。得到的3个克隆,经克隆杂交及电泳分析后,证实分别带有上述3个目的片段。这些特异片段的特性及其与花粉育性的关系尚在研究中。 相似文献
95.
Zhiwei Wang Aamir Ahmad Yiwei LiDejuan Kong Asfar S. AzmiSanjeev Banerjee Fazlul H. Sarkar 《生物化学与生物物理学报:癌评论》2010
Platelet-derived growth factor-D (PDGF-D) can regulate many cellular processes, including cell proliferation, apoptosis, transformation, migration, invasion, angiogenesis and metastasis. Therefore PDGF-D signaling has been considered to be important in human malignancies, and thus PDGF-D signaling may represent a novel therapeutic target, and as such suggests that the development of agents that will target PDGF-D signaling is likely to have a significant therapeutic impact on human cancers. This mini-review describes the mechanisms of signal transduction associated with PDGF-D signaling to support the role of PDGF-D in the carcinogenesis. Moreover, we summarize data on several PDGF-D inhibitors especially naturally occurring “chemopreventive agent” such an indole compound, which we believe could serve as a novel agent for the prevention of tumor progression and/or treatment of human malignancies by targeted inactivation of PDGF-D signaling. 相似文献
96.
植物凝集素(PHA)对小鼠白细胞及血清乳酸脱氢酶同工酶的影响 总被引:1,自引:0,他引:1
本文就 PHA 在不同时间内对小鼠白细胞及血清 LDH,同工酶的影响进行了观察,结果表明,PHA 对小鼠白细胞及血清的 LDH 酶活性有一定的影响,24h,48h,72h 三个不同时间组均与对照组有显著性差异(P<0.01),其中以48h 组酶活性最强,同时淋巴细胞的酶活性明显高于粒细胞;血清同工酶谱也有明显的变化,LDH_(1-4)均低于正常(P<0.01),LDH_5明显增高(P<0.01),同时出现了 LDH_5(?)亚带. 相似文献
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98.
甜樱桃品种SSR-PCR反应体系的优化 总被引:1,自引:0,他引:1
以甜樱桃品种那翁为试材,研究了樱桃SSR技术中PCR反应体系的主要成分对SSR扩增结果的影响,并比较了采用聚丙稀酰胺凝胶及琼脂糖电泳检测扩增产物多态性的差异.结果表明:在PCR反应体系中,DNA最适浓度30~45 ng;Mg2+的最适浓度范围为1.5~3.0 mmol/L;dNTP最适浓度为0.2~0.3 mmol/L;引物的最适浓度为0.3~0.4 μmol/L;Taq聚合酶在20 μl反应体系中宜加入0.5 U.利用此反应体系,对24份樱桃代表资源进行了SSR反应,用6%的非变性聚丙稀酰胺凝胶电泳检测,扩增产物在100~250 bp之间,不同品种间DNA谱带多态性丰富.琼脂糖电泳检测的DNA多态性不如聚丙稀酰胺凝胶丰富. 相似文献
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100.
Angiogenin (Ang) is known to induce cell proliferation and inhibit apoptosis by cellular signaling pathways and by direct nuclear functions of Ang, but the mechanism of action for Ang is not yet clear. The aim of present study was to identify novel binding partner of Ang and to explore the underlying mechanism. With the use of yeast two-hybrid screening system, Ang was used as the bait to screen human fetal hepatic cDNA library for interacting proteins. Four and a half LIM domains 3 (FHL3) was identified as a novel Ang binding partner. The interaction between Ang and the full length FHL3 was further confirmed by yeast two-hybrid assay, co-immunoprecipitation and GST pull-down assays. Furthermore, FHL3 was required for Ang-mediated HeLa cell proliferation and nuclear translocation of Ang. These findings suggest that the interaction between Ang and FHL3 may provide some clues to the mechanisms of Ang-regulated cell growth and apoptosis. 相似文献