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Qing-yi Xie Xiao-dong Bao Qing-yu Ma Fan-dong Kong Man-li Zhou Bing Yan You-xing Zhao 《Antonie van Leeuwenhoek》2018,111(1):125-133
An actinomycete strain, 2603PH03T, was isolated from a mangrove rhizosphere soil sample collected in Wenchang, China. Phylogenetic analysis of the 16S rRNA gene sequence of strain 2603PH03T indicated high similarity to Verrucosispora gifthornensis DSM 44337T (99.4%), Verrucosispora andamanensis (99.4%), Verrucosispora fiedleri MG-37T (99.4%) and Verrucosispora maris AB18-032T (99.4%). The cell wall was found to contain meso-diaminopimelic acid and glycine. The major menaquinones were identified as MK-9(H4), MK-9(H6) and MK-9(H8), with MK-9(H2), MK-10(H2), MK-9(H10) and MK-10(H6) as minor components. The characteristic whole cell sugars were found to be xylose and mannose. The phospholipid profile was found to contain phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside, phosphatidylinositol, phosphatidylserine and an unidentified phospholipid. The DNA G+C content was determined to be 70.1 mol%. The results of physiological and biochemical tests and low DNA-DNA relatedness readily distinguished the isolate from the closely related species. On the basis of these phenotypic and genotypic data, strain 2603PH03T is concluded to represent a novel species of the genus Verrucosispora, for which the name Verrucosispora rhizosphaerae sp. nov. is proposed. The type strain is 2603PH03T (=CCTCC AA 2016023T = DSM 45673T). 相似文献
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Wildfire is one of the most important global agents of disturbance affecting terrestrial and riparian vegetation. Post-fire vegetation changes can alter stream resource pathways and cause channel reorganization and sediment-laden debris flows. Yet, little is known about macroinvertebrate community recovery following wildfire and debris flows and how these communities fit into the broader stream community mosaic. We examined the effects of wildfire and debris flows on relative resource availability and macroinvertebrate assemblages at 31 streams in Idaho, USA using a space-for-time study design. Wildfire and debris flows had no apparent effects on resource standing crop. However, macroinvertebrate communities among unburned, burned, and debris flow streams were quite different. Compared to unburned streams, biomass and density were higher at streams which experienced debris flows ~ 10 years post fire, but exhibited the near-complete absence of macroinvertebrates at streams with more recent debris flows. Stream macroinvertebrate communities impacted by debris flows were distinct compared to unburned and burned streams which did not experience debris flows. When found, differences in macroinvertebrate biomass, density, richness, and community structures were largely due to the incidence of debris flows. Debris flows removed the riparian vegetation, slowing its recovery, cascading to affect macroinvertebrate community structure into the long term. 相似文献
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Jing Kong Yuanjie Dong Yiling Song Xiaoying Bai Xianyi Tian Linlin Xu Shuang Liu Zhenli He 《Journal of Plant Growth Regulation》2016,35(1):31-43
A greenhouse hydroponic experiment was performed to evaluate how peanut seedlings (Arachis hypogaea L.) responded to iron (Fe) deficiency stress in the presence of sodium nitroprusside (SNP), a nitric oxide (NO) donor. The results showed that Fe deficiency inhibited peanut plant growth, decreased chlorophyll and active Fe concentrations, and dramatically disturbed ion balance. The addition of 50, 100, 250, and 500 µM SNP, significantly promoted the absorption of Fe in the cell wall, cell organelles, and soluble fractions, increased the concentrations of active Fe and chlorophyll in peanut plants, and alleviated the excess absorption of manganese (Mn) and copper (Cu) induced by Fe deficiency. In addition, SNP also significantly increased the activities of superoxide dismutase, peroxidase, and catalase, which is beneficial to inhibit the accumulation of malondialdehyde and reactive oxygen species. Addition of 250 µM SNP had the most significant alleviating effect against Fe-deficiency stress, and after 15 days of treatment, the plants with the 250 µM SNP treatment achieved comparable NO levels with those grown under optimal nutrition conditions. However, the effects of SNP were reversed by addition of hemoglobin (Hb, a NO scavenger). These results suggest that NO released from SNP decomposition was responsible for the effect of SNP-induced alleviation on Fe deficiency. 相似文献
858.
Yancun Yin Hui Hua Minjing Li Shu Liu Qingbin Kong Ting Shao Jiao Wang Yuanming Luo Qian Wang Ting Luo Yangfu Jiang 《Cell research》2016,26(1):46-65
Mammalian target of rapamycin (mTOR) is a core component of raptor-mTOR (mTORC1) and rictor-mTOR (mTORC2) complexes that control diverse cellular processes. Both mTORC1 and mTORC2 regulate several elements downstream of type I insulin-like growth factor receptor (IGF-IR) and insulin receptor (InsR). However, it is unknown whether and how mTOR regulates IGF-IR and InsR themselves. Here we show that mTOR possesses unexpected tyrosine kinase activity and activates IGF-IR/InsR. Rapamycin induces the tyrosine phosphorylation and activation of IGF-IR/InsR, which is largely dependent on rictor and mTOR. Moreover, mTORC2 promotes ligand-induced activation of IGF-IR/InsR. IGF- and insulin-induced IGF-IR/InsR phosphorylation is significantly compromised in rictor-null cells. Insulin receptor substrate (IRS) directly interacts with SIN1 thereby recruiting mTORC2 to IGF-IR/InsR and promoting rapamycin- or ligand-induced phosphorylation of IGF-IR/InsR. mTOR exhibits tyrosine kinase activity towards the general tyrosine kinase substrate poly(Glu-Tyr) and IGF-IR/InsR. Both recombinant mTOR and immunoprecipitated mTORC2 phosphorylate IGF-IR and InsR on Tyr1131/1136 and Tyr1146/1151, respectively. These effects are independent of the intrinsic kinase activity of IGF-IR/InsR, as determined by assays on kinase-dead IGF-IR/InsR mutants. While both rictor and mTOR immunoprecitates from rictor+/+ MCF-10A cells exhibit tyrosine kinase activity towards IGF-IR and InsR, mTOR immunoprecipitates from rictor−/− MCF-10A cells do not induce IGF-IR and InsR phosphorylation. Phosphorylation-deficient mutation of residue Tyr1131 in IGF-IR or Tyr1146 in InsR abrogates the activation of IGF-IR/InsR by mTOR. Finally, overexpression of rictor promotes IGF-induced cell proliferation. Our work identifies mTOR as a dual-specificity kinase and clarifies how mTORC2 promotes IGF-IR/InsR activation. 相似文献
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