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61.
Sara Alberghini Elisa Polone Viviana Corich Milena Carlot Flavio Seno Antonio Trovato & Andrea Squartini 《FEMS microbiology letters》2009,292(2):149-161
Cell-to-cell bacterial communication via diffusible signals is addressed and the conceptual framework in which quorum sensing is usually described is evaluated. By applying equations ruling the physical diffusion of the autoinducer molecules, one can calculate the gradient profiles that would occur either around a single cell or at the center of volumes of increasing size and increasing cell densities. Water-based matrices at 25 °C and viscous biofilms at colder temperatures are compared. Some basic consequences relevant for the field of microbial signalling arise. As regards induction, gradient-mixing dynamics between as little as two cells lying at a short distance appears to be sufficient for the buildup of a concentration reaching the known thresholds for quorum sensing. A straight line in which the highest concentrations occur is also created as a consequence of the gradient overlap geometry, providing an additional signal information potentially useful for chemotactic responses. In terms of whole population signalling, it is shown how the concentration perceived by a cell in the center is critically dependent not only on the cell density but also on the size of the biofilm itself. Tables and formulas for the practical prediction of N -acyl homoserine lactones concentrations at desired distances in different cell density biofilms are provided. 相似文献
62.
Joshua M. Thurman Maria Wong Brandon Renner Ashley Frazer-Abel Patricia C. Giclas Melanie S. Joy Diana Jalal Milena K. Radeva Jennifer Gassman Debbie S. Gipson Frederick Kaskel Aaron Friedman Howard Trachtman 《PloS one》2015,10(9)
Background
Recent pre-clinical studies have shown that complement activation contributes to glomerular and tubular injury in experimental FSGS. Although complement proteins are detected in the glomeruli of some patients with FSGS, it is not known whether this is due to complement activation or whether the proteins are simply trapped in sclerotic glomeruli. We measured complement activation fragments in the plasma and urine of patients with primary FSGS to determine whether complement activation is part of the disease process.Study Design
Plasma and urine samples from patients with biopsy-proven FSGS who participated in the FSGS Clinical Trial were analyzed.Setting and Participants
We identified 19 patients for whom samples were available from weeks 0, 26, 52 and 78. The results for these FSGS patients were compared to results in samples from 10 healthy controls, 10 patients with chronic kidney disease (CKD), 20 patients with vasculitis, and 23 patients with lupus nephritis.Outcomes
Longitudinal control of proteinuria and estimated glomerular filtration rate (eGFR).Measurements
Levels of the complement fragments Ba, Bb, C4a, and sC5b-9 in plasma and urine.Results
Plasma and urine Ba, C4a, sC5b-9 were significantly higher in FSGS patients at the time of diagnosis than in the control groups. Plasma Ba levels inversely correlated with the eGFR at the time of diagnosis and at the end of the study. Plasma and urine Ba levels at the end of the study positively correlated with the level of proteinuria, the primary outcome of the study.Limitations
Limited number of patients with samples from all time-points.Conclusions
The complement system is activated in patients with primary FSGS, and elevated levels of plasma Ba correlate with more severe disease. Measurement of complement fragments may identify a subset of patients in whom the complement system is activated. Further investigations are needed to confirm our findings and to determine the prognostic significance of complement activation in patients with FSGS. 相似文献63.
64.
65.
Milena Gisela Casafús Marília Melo Favalesso Micaela Andrea Gritti Juan Manuel Coronel Ana Tereza Bittencourt Guimares Maria Elisa Peichoto 《PLoS neglected tropical diseases》2021,15(7)
BackgroundEnvenomation by the South American Lonomia saturniid caterpillars, named lonomism, constitutes an emerging and somewhat neglected public health issue in Argentina and neighboring countries. Considering that there is an intricate relationship between environment and human health in such cases, this study aimed to analyze the eco-epidemiological profile of 40 accidents and 33 occurrences of Lonomia spp. in Misiones (Argentina) between January 2014 and May 2020.Methodology/Principal findingsWe described the eco-epidemiological variables and characterized the abiotic scenario of such cases. Additionally, we obtained a density map that shows the punctual intensity of Lonomia records throughout Misiones. Most of the accidents occurred in the Department of Guaraní and involved male victims younger than 20 years old. The accidental/occasional occurrence of Lonomia spp. (considering both adult and caterpillar stages together) was significantly higher in the rural area, whereas only adult specimens were found in urban areas. We determined that the presence of this insect in Misiones is positively related to higher temperatures and solar radiation, and larger precipitation and evapotranspiration throughout the year.Conclusion/SignificanceThis study represents an initial step towards the global understanding of lonomism as a public health problem in Argentina. It provides a map of the risk level for this envenomation in Misiones, which could help authorities address public health policy efforts to implement sustainable strategies for prevention and response to this threat in Northeastern Argentina and neighboring regions. 相似文献
66.
Yeasts isolated from olive mill wastewaters from southern Italy: technological characterization and potential use for phenol removal 总被引:1,自引:0,他引:1
Milena Sinigaglia Nilde Di Benedetto Antonio Bevilacqua Maria Rosaria Corbo Angela Capece Patrizia Romano 《Applied microbiology and biotechnology》2010,87(6):2345-2354
Olive mill wastewater (OMW) samples from two traditional varieties (Peranzana and Ogliarola Garganica) of Apulian region (southern
Italy) and produced through continuous and traditional methods were microbiologically and chemically examined; thus, 104 yeasts
were isolated and selected for further analyses. The strains were identified as Candida boidinii, Pichia holstii, Pichia membranifaciens, and Saccharomyces cerevisiae and analyzed to assess their suitability to metabolize phenols. Based on phenol metabolism, 27 strains were selected and
inoculated into OMW aliquots to determine their ability to reduce phenols in vivo; then, five strains (identified with the
codes 682—C. boidinii and 625, 642, 647, and 941—P. holstii) were used as a cocktail in wastewaters for a final validation step. In this last experiment, the effects of the temperature
(10–30°C) and (NH4)2SO4 (0.0–6.0 g l−1) were studied through a central composite design approach, and the results highlighted that the cocktail was able to reduce
phenols by 40% at 10°C with 6.0 g l−1 of (NH4)2SO4 added. 相似文献
67.
Influence of Cross-linked Waxy Maize Starch on the Aggregation Behavior of Casein Micelles During Acid-induced Gelation 总被引:1,自引:0,他引:1
The influence of cross-linked waxy maize starch on the aggregation behavior of casein micelles was investigated using a combination
of physico-chemical techniques. Milk was homogenized at two different temperatures (55 and 65 °C) and then heated at 95 °C
for 5 min in a pilot scale system. The possible interactions between modified starch and milk proteins during lactic acid
fermentation were evaluated. While 1% starch did not show differences in the whey protein complexes formed during heating
compared to milk with no starch (as measured by size exclusion chromatography), a higher (2.5%) concentration of starch clearly
showed an increased amount of heat-induced whey protein aggregates. The gelation pH also increased significantly with 2.5%
starch compared to that of the control samples. The storage modulus (G′) increased with increasing levels of starch, and confocal
microscopy confirmed that the microstructure of the casein gels was altered by the presence of modified starch. Milk-starch
mixtures preheated and homogenized at 55 or 65 °C exhibited similar physico-chemical behavior during acidification. The results
suggested a lack of interaction between starch granules and casein micelles during acidification, and scanning electron microscopy
images collected with a self-assembled monolayer technique also confirmed that starch granules were not attached to milk caseins
but only embedded in the protein gel matrix. 相似文献
68.
Nacira Darghal Nadia Bouchemal Carlos F.G.C. Geraldes Milena Salerno 《Journal of inorganic biochemistry》2010,104(1):47-54
Alzheimer’s disease (AD) is the most commonly form of dementia in the elderly. The development of molecules able to detect biomarkers characteristic of AD is critical to its understanding and treatment. However, such molecules must be able to pass blood-brain barrier (BBB) which is a major impediment to the entry of many therapeutic drugs into the brain. Such a limitation applies to the development of magnetic resonance imaging molecular neuroimaging agents using biomarkers of AD-like β-amyloid deposits, as the common extracellular contrast agents (CAs) are not able to cross an intact BBB. In this work, we have studied the ability of a series of simple Eu3+ complexes to enter cells overexpressing or not the ABCB1 (P-gp or P-glycoprotein) protein, which is expressed at the BBB and in human embryonic astrocytes. The intracellular uptake of the Eu3+ complexes of linear and macrocyclic polyaminocarboxylate ligands with different charges and lipophilicities was followed by atomic absorption spectrometry. Based on biochemical argument, we propose that lipophilic contrast agents can be efficiently taken up by cells and accumulate inside mitochondria when they are positively charged. The important point is that they are not P-gp substrates, which is one of the major obstacles for them to cross the BBB. 相似文献
69.
Yongsheng Chen Imad Zein Everton Alen Brenner Jeppe Reitan Andersen Mathias Landbeck Milena Ouzunova Thomas Lübberstedt 《BMC plant biology》2010,10(1):12
Background
Reduced lignin content leads to higher cell wall digestibility and, therefore, better forage quality and increased conversion of lignocellulosic biomass into ethanol. However, reduced lignin content might lead to weaker stalks, lodging, and reduced biomass yield. Genes encoding enzymes involved in cell wall lignification have been shown to influence both cell wall digestibility and yield traits. 相似文献70.
Śárka Králíková Miloś Buděšínský Milena Masojídková Ivan Rosenberg 《Nucleosides, nucleotides & nucleic acids》2013,32(7):1159-1183
Abstract In this report we present a novel, simple way for the synthesis of 3′-C-phosphonate derivatives of all four basic 2′-deoxynucleosides in both fully protected and deprotected forms. The reactivity of the geminal hydroxy phosphonate moiety located at the 3′-carbon atom of the nucleoside was studied with respect to the use of this type of nucleoside phosphonic acid for the preparation of short oligonucleotides, namely, dinucleoside monophosphate analogues. 相似文献