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81.
Svetlana P Chapoval Koji Iijima Eric V Marietta Michele K Smart Andrei I Chapoval Amy G Andrews Chella S David 《Journal of immunology (Baltimore, Md. : 1950)》2002,168(2):890-899
To investigate the role of HLA-DQ molecules and/or CD4(+) T cells in the pathogenesis of allergic asthma, we generated HLA-DQ6 and HLA-DQ8 transgenic mice lacking endogenous class II (Abeta(null)) and CD4 genes and challenged them intranasally with short ragweed allergenic extract (SRW). We found that DQ6/CD4(null) mice developed a strong eosinophilic infiltration into the bronchoalveolar lavage and lung tissue, while DQ8/CD4(null) mice were normal. However, neither cytokines nor eosinophil peroxidase in the bronchoalveolar lavage of DQ6/CD4(null) mice was found. In addition, the airway reactivity to methacholine was elevated moderately in DQ6/CD4(null) mice compared with the high response in DQ/CD4(+) counterparts and was only partially augmented by CD4(+) T cell transfer. The DQ6/CD4(null) mice showed Th1/Th2-type cytokines and SRW-specific Abs in the immune sera in contrast to a direct Th2 response observed in DQ6/CD4(+) mice. The proliferative response of spleen mononuclear cells and peribronchial lymph node cells demonstrated that the response to SRW in DQ6/CD4(null) mice was mediated by HLA-DQ-restricted CD4(-)CD8(-)NK1.1(-) T cells. FACS analysis of PBMC and spleen mononuclear cells demonstrated an expansion of double-negative (DN) CD4(-)CD8(-)TCRalphabeta(+) T cells in SRW-treated DQ6/CD4(null) mice. These cells produced IL-4, IL-5, IL-13, and IFN-gamma when stimulated with immobilized anti-CD3. IL-5 ELISPOT assay revealed that DN T cells were the cellular origin of IL-5 in allergen-challenged DQ6/CD4(null) mice. Our study shows a role for HLA-DQ-restricted CD4(+) and DN T cells in the allergic response. 相似文献
82.
Koji Okamoto 《FEMS microbiology letters》1986,37(3):383-385
Abstract Using a shaking culture system, we have previously shown that both cell contact and cAMP are required for pre-spore differentiation in Dictyostelium discoideum [2]. In the present study, cAMP was removed from the medium by the use of a hydrolysing enzyme after cells had formed agglomerates. This treatment left the agglomerates unchanged, but caused a rapid decrease in the activity of UDP galactose transferase, a pre-spore-specific enzyme. This result indicates that cAMP is required even after agglomerate formation to maintain pre-spore differentiation. 相似文献
83.
A phytase (EC 3.1.3.8) with a high affinity for phytic acid was found in Aspergillus niger SK-57 and purified to homogeneity in four steps by using ion-exchange chromatography (two types), gel filtration, and chromatofocusing. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the purified enzyme gave a single stained band at a molecular mass of approximately 60 kDa. The Michaelis constant of the enzyme for phytic acid (18.7 +/- 4.6 microM) was statistically analyzed. In regard to the orthophosphate released from phytic acid, a significant difference between a low K(m) phytase from A. niger SK-57 and a high K(m) phytase from Aspergillus ficuum was recognized. 相似文献
84.
Kenta Watanabe Koji Seike Rumiko Kajihara Shigeru Montani Tomohiro Kuwae 《Global Change Biology》2019,25(3):1063-1077
Because coastal habitats store large amounts of organic carbon (Corg), the conservation and restoration of these habitats are considered to be important measures for mitigating global climate change. Although future sea‐level rise is predicted to change the characteristics of these habitats, its impact on their rate of Corg sequestration is highly uncertain. Here we used historical depositional records to show that relative sea‐level (RSL) changes regulated Corg accumulation rates in boreal contiguous seagrass–saltmarsh habitats. Age–depth modeling and geological and biogeochemical approaches indicated that Corg accumulation rates varied as a function of changes in depositional environments and habitat relocations. In particular, Corg accumulation rates were enhanced in subtidal seagrass meadows during times of RSL rise, which were caused by postseismic land subsidence and climate change. Our findings identify historical analogs for the future impact of RSL rise driven by global climate change on rates of Corg sequestration in coastal habitats. 相似文献
85.
Bink RJ Habuchi H Lele Z Dolk E Joore J Rauch GJ Geisler R Wilson SW den Hertog J Kimata K Zivkovic D 《The Journal of biological chemistry》2003,278(33):31118-31127
Heparan sulfate proteoglycans function in development and disease. They consist of a core protein with attached heparan sulfate chains that are altered by a series of carbohydrate-modifying enzymes and sulfotransferases. Here, we report on the identification and characterization of a gene encoding zebrafish heparan sulfate 6-O-sulfotransferase (hs6st) that shows high homology to other heparan sulfate 6-O-sulfotransferases. When expressed as a fusion protein in cultured cells, the protein shows specific 6-O-sulfotransferase activity and preferentially acts on the iduronosyl N-sulfoglycosamine. In the developing embryo, hs6st is expressed in the brain, the somites, and the fins; the same structures that were affected upon morpholino-mediated functional knockdown. Morpholino injections significantly inhibited 6-O- but not 2-O-sulfation as assessed by HPLC. Morphants display disturbed somite specification independent of the somite oscillator mechanism and have impaired muscle differentiation. In conclusion, our results show that transfer of sulfate to specific positions on glycosaminoglycans is essential for muscle development. 相似文献
86.
Shigeko Ishimatsu Toshihiro Kawamoto Koji Matsuno Yasushi Kodama 《Biological trace element research》1995,49(1):43-52
In this study, eight kinds of nickel (Ni) compounds were orally administered to Wistar male rats and the distribution of each
compound was investigated 24 h after the administration. The Ni compounds used in this experiment were nickel metal [Ni−M],
nickel oxide (green) [NiO(G)], nickel oxide (black) [NiO(B)], nickel subsulfide [Ni3S2], nickel sulfide [NiS], nickel sulfate [NiSO4], nickel chloride [NiCl2], and nickel nitrate [Ni(NO3)2]. The solubilities of the nickel compounds in saline solution were in the following order; [Ni(NO3)2>NiCl2>NiSO4]≫[NiS>Ni3S2]>[NiO(B)>Ni−M>NiO(G)]. The Ni level in the visceral organs was higher in the rats given soluble Ni compounds; Ni(NO3)2, NiCl2, NiSO4, than that in the rats receiving other compounds. In the rats to which soluble Ni compounds were administered, 80–90% of
the recovered Ni amounts in the examined organs was detected in the kidneys. On the other hand, the Ni concentration in organs
administered scarcely soluble Ni compounds; NiO(B), NiO(G), and Ni−M were very low. The estimated absorbed fraction of each
Ni compounds was increased with the increase of the solubility. These results suggest that the kinetic behavior of Ni compounds
administered orally is closely related with the solubility of Ni compounds, and that the solubility of Ni compounds is one
of the important factors for determining the health effect of Ni compounds. 相似文献
87.
Y. Kobayashi Yufuko Takahashi Satoshi Chikayama Motomi Ikeda Nobuhiko Uoshima Shinya Kimura Koji Tanaka Katuya Wada Masaru Ozawa Tatuo Sugano Naoyuki Maruo Motoharu Kondo 《Histochemistry and cell biology》1997,108(2):115-120
We devised a new microfluorometric method for determining the ploidy of megakaryocytes identified immunologically in bone
marrow smears. The smears were immunostained by incubation with mouse monoclonal anti-glycoproteins (GP) IIb antibodies, followed
by fluorescein isothiocyanate-conjugated goat anti-mouse IgG antibodies. They were then stained with 4′,6-diamidino-2-phenylindole
(DAPI). Megakaryocytes were identified by their GPIIb immunofluorescence using a microfluorometer and, after the filters were
changed, their DNA content was assayed by measuring the intensity of DAPI fluorescence. This intensity was shown to be proportional
to the DNA content when the aperture of the objective lens was reduced. We compared these results with those obtained when
megakaryocytes were identified morphologically, using DAPI staining after Wright-Giemsa destaining. In all 12 normal controls,
the ploidy peaks were shown to be 16N by both methods, and the mean ploidy detected by the immunological method was only reduced
0.961 times relative to the estimate from the morphological method. In contrast, in eight myelodysplastic syndrome (MDS) patients,
the ploidy peaks were either 8N or 4N and the mean was reduced by 0.906 times (P=0.018). Thus we could immunologically identify small megakaryocytes which we could not identify morphologically. Therefore,
this method is useful for measuring megakaryocytic ploidy, especially in the pathological megakaryocytes of MDS patients.
Accepted: 29 April 1997 相似文献
88.
Effects of lactoferrin and lactoperoxidase‐containing food on the oral microbiota of older individuals 下载免费PDF全文
Manabu Nakano Hiroyuki Wakabayashi Hirosuke Sugahara Toshitaka Odamaki Koji Yamauchi Fumiaki Abe Jin‐Zhong Xiao Kohji Murakami Kentaro Ishikawa Shouji Hironaka 《Microbiology and immunology》2017,61(10):416-426
89.
Epigenetic mechanisms such as DNA methylation or histone modifications are essential for the regulation of gene expression and development of tissues. Alteration of epigenetic modifications can be used as an epigenetic biomarker for diagnosis and as promising targets for epigenetic therapy. A recent study explored cancer-cell specific epigenetic biomarkers by examining different types of epigenetic modifications simultaneously. However, it was based on microarrays and reported biomarkers that were also present in normal cells at a low frequency. Here, we first analyzed multi-omics data (including ChIP-Seq data of six types of histone modifications: H3K27ac, H3K4me1, H3K9me3, H3K36me3, H3K27me3, and H3K4me3) obtained from 26 lung adenocarcinoma cell lines and a normal cell line. We identified six genes with both H3K27ac and H3K4me3 histone modifications in their promoter regions, which were not present in the normal cell line, but present in ≥85% (22 out of 26) and ≤96% (25 out of 26) of the lung adenocarcinoma cell lines. Of these genes, NUP210 (encoding a main component of the nuclear pore complex) was the only gene in which the two modifications were not detected in another normal cell line. RNA-Seq analysis revealed that NUP210 was aberrantly overexpressed among the 26 lung adenocarcinoma cell lines, although the frequency of NUP210 overexpression was lower (19.3%) in 57 lung adenocarcinoma tissue samples studied and stored in another database. This study provides a basis to discover epigenetic biomarkers highly specific to a certain cancer, based on multi-omics data at the cell population level. 相似文献
90.
Ogawa S Yamakawa H Yamanoi J Nishida S Kano Y Takeshima T Tauchi K Nagashima H 《Theriogenology》1988,29(5):1083-1089
An attempt was made to detect the fluorescent bodies (F-body), using Quinacrine mustard (Q-M) staining in the spermatozoa from eight mammalian species (human, bull, boar, dog, rabbit, rat, mouse, and mastomys) as well as in the cock (used as negative control). Sperm suspension, prepared after rinsing by repeated centrifugation with phosphate buffered saline (PBS), was either stained with Q-M for 24 h or treated with protease and then stained with Q-M for 60 min. The final concentration of Q-M in the mixed staining sperm suspension was 0.025 mg/ml. The examination using a reflecting fluorescent microscope revealed that the F-body found in human sperm was also present in the sperm of all the mammals but not in the cock after 24 h of staining. The enzyme-treated specimens showed higher incidences of F-bodies than specimens stained for 24 h without enzymatic digestion. These findings strongly suggest that the F-body is commonly present in the spermatozoa of many mammalian species. 相似文献