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971.
Hironori Toyama Kazuhiro Bessho Liangliang Huang Shun K. Hirota Yuichi Kano Keiko Mase Tatsuro Sato Akiyo Naiki Jianhua Li Yukihiro Shimatani Tetsukazu Yahara 《Freshwater Biology》2020,65(4):632-645
- Water pollution is one of the most serious aquatic environmental problems worldwide. In China, recent agricultural and industrial development has resulted in rapid changes in aquatic ecosystems. Here, we reveal the effects of water pollution on the phylogenetic community structure of aquatic macrophytes in the Tiaoxi River, China.
- We placed a rectangular plot at 47 sites within the Tiaoxi River from the mouth of the river to 88.5 km upstream, in which we recorded species abundance and measured 22 physico-chemical variables. Bayesian phylogeny using the rbcL and matK gene sequences was employed to quantify phylogenetic α- and β-diversity, and test the phylogenetic signal in four growth forms: emergent, floating-leaved, free-floating, and submerged.
- Within communities, water contamination and phytoplankton abundance decreased species richness and phylogenetic diversity, which resulted in phylogenetic clustering; species within communities were more closely related to each other than expected. Between communities, differences in geographical distance and phytoplankton abundance resulted in phylogenetic dissimilarity among plots. Aquatic macrophytes showed phylogenetic signals in which related species responded more similarly to disturbance.
- Thus, the observed patterns could be explained by environmental filtering and suggested that water pollution by human activity has added more filters to the existing environmental filters that drive the species assembly of macrophyte communities.
972.
973.
Hitoshi Murata Akiyoshi Yamada Satoru Yokota Tsuyoshi Maruyama Naoki Endo Kohei Yamamoto Tatsuro Ohira Hitoshi Neda 《Mycorrhiza》2014,24(4):315-321
We previously reported that Tricholoma matsutake and Tricholoma fulvocastaneum, ectomycorrhizal basidiomycetes that associate with Pinaceae and Fagaceae, respectively, in the Northern Hemisphere, could interact in vitro as a root endophyte of somatic plants of Cedrela odorata (Meliaceae), which naturally harbors arbuscular mycorrhizal fungi in South America, to form a characteristic rhizospheric colony or “shiro”. We questioned whether this phenomenon could have occurred because of plant–microbe interactions between geographically separated species that never encounter one another in nature. In the present study, we document that these fungi formed root endophyte interactions and shiro within 140 days of inoculation with somatic plants of Prunus speciosa (=Cerasus speciosa, Rosaceae), a wild cherry tree that naturally harbors arbuscular mycorrhizal fungi in Japan. Compared with C. odorata, infected P. speciosa plants had less mycelial sheath surrounding the exodermis, and the older the roots, especially main roots, the more hyphae penetrated. In addition, a large number of juvenile roots were not associated with hyphae. We concluded that such root endophyte interactions were not events isolated to the interactions between exotic plants and microbes but could occur generally in vitro. Our pure culture system with a somatic plant allowed these fungi to express symbiosis-related phenotypes that varied with the plant host; these traits are innately programmed but suppressed in nature and could be useful in genetic analyses of plant–fungal symbiosis. 相似文献
974.
Tadashi Kasahara Hirotomo Harada Kenji Ogata Tadashi Kawai Kohei Shioiri-Nakano 《Microbiology and immunology》1981,25(12):1345-1356
Significant immunoglobulin (Ig) production by human peripheral blood lymphocytes was induced in vitro by stimulating the cells with pokeweed mitogen (PWM) and Staphylococcus aureus Cowan I (SpA CoI). IgG, IgM, and IgA were determined by a combination of the latex fixation test and radioimmunoassay. High levels (1,000 to 5,000 μg/ml) of IgG and IgM and a lesser amount of IgA were constantly produced during 7 to 8 days of incubation with both stimulants. Ig production induced by SpA Col stimulation was independent of the presence of T cells, while Ig production induced by PWM required T cells exclusively. Depletion of monocytes in the culture caused but a slight decrease in Ig production (particularly in the case of IgG). While the addition of a small number of monocytes enhanced IgG induction by both stimulants, coculture with an excess number of monocytes inhibited Ig induction (particularly IgG) by PWM stimulation but not by SpA CoI stimulation. Marked suppression of Ig production (IgG, IgM, and IgA) was observed in cocultures with Con A-activated T cells. The phenomena of suppression were observed in both the SpA Col-stimulated and PWM-stimulated lymphocytes. These data indicate that Ig production from B cells stimulated with a polyclonal B cell activator, SpA CoI, was independent of T cells and relatively of independent of monocytes, but could be subjected to the regulation of the Con A-induced suppressor T cells. 相似文献
975.
976.
Haruka Ishihara Kohei Ogura Tohru Miyoshi-Akiyama Masahiko Nakamura Hiroyasu Kaya Shigefumi Okamoto 《Microbiology and immunology》2020,64(2):113-122
Streptococcus dysgalactiae subsp. equisimilis (SDSE) causes severe invasive streptococcal infections, especially in elderly people. Between 2013 and 2018, 88 streptococci were isolated from clinical blood culture in a hospital in Toyama prefecture, Japan. The collection included six Group A SDSE (ASD) strains, which are rarely isolated. Multilocus sequence typing categorized five of the six strains into ST128 and the remaining strain into a new type. Maximum-likelihood phylogenetic analysis revealed that the six ASD strains had highly similar genome sequences. Bayesian analysis indicated that the most recent common ancestor of the strains appeared 39 years ago. The ASD strains possessed carbohydrate synthase genes that are conserved in Streptococcus pyogenes strains, whereas one strain featured a different arrangement of the gene cluster. The carbohydrate synthase genes varied by Lancefield type (A, C, and G). 相似文献
977.
Natsuko Saito Shunsuke Kimura Tomomi Miyamoto Sanae Fukushima Misato Amagasa Yoshinori Shimamoto Chieko Nishioka Shiki Okamoto Chitoku Toda Kohei Washio Atsushi Asano Ichiro Miyoshi Eiki Takahashi Hiroshi Kitamura 《Biochemistry and Biophysics Reports》2017
We previously reported that ubiquitin-specific protease (USP) 2 in macrophages down-regulates genes associated with metabolic diseases, suggesting a putative anti-diabetic role for USP2 in macrophages. In this study, we evaluate this role at both cellular and individual levels. Isolated macrophages forcibly expressing Usp2a, a longer splicing variant of USP2, failed to modulate the insulin sensitivity of 3T3-L1 adipocytes. Similarly, macrophage-selective overexpression of Usp2a in mice (Usp2a transgenic mice) had a negligible effect on insulin sensitivity relative to wild type littermates following a three-month high-fat diet. However, Usp2a transgenic mice exhibited fewer M1 macrophages in their mesenteric adipose tissue. Following a six-month high-fat diet, Usp2a transgenic mice exhibited a retarded progression of insulin resistance in their skeletal muscle and liver, and an improvement in insulin sensitivity at an individual level. Although conditioned media from Usp2a-overexpressing macrophages did not directly affect the insulin sensitivity of C2C12 myotubes compared to media from control macrophages, they did increase the insulin sensitivity of C2C12 cells after subsequent conditioning with 3T3-L1 cells. These results indicate that macrophage USP2A hampers obesity-elicited insulin resistance via an adipocyte-dependent mechanism. 相似文献
978.
Direct production of 4-hydroxybenzoic acid from cellulose using cellulase-displaying Pichia pastoris
Kentaro Inokuma Shunya Miyamoto Kohei Morinaga Yuma Kobayashi Ryota Kumokita Takahiro Bamba Yoichiro Ito Akihiko Kondo Tomohisa Hasunuma 《Biotechnology and bioengineering》2023,120(4):1097-1107
4-hydroxybenzoic acid (4-HBA) is an industrially important aromatic compound, and there is an urgent need to establish a bioprocess to produce this compound in a sustainable and environmentally friendly manner from renewable feedstocks such as cellulosic biomass. Here, we developed a bioprocess to directly produce 4-HBA from cellulose using a recombinant Pichia pastoris strain that displays heterologous cellulolytic enzymes on its cell surface via the glycosylphosphatidylinositol (GPI)-anchoring system. β-glucosidase (BGL) from Aspergillus aculeatus, endoglucanase (EG) from Trichoderma reesei, and cellobiohydrolase (CBH) from Talaromyces emersonii were co-displayed on the cell surface of P. pastoris using an appropriate GPI-anchoring domain for each enzyme. The cell-surface cellulase activity was further enhanced using P. pastoris SPI1 promoter- and secretion signal sequences. The resulting strains efficiently hydrolyzed phosphoric acid swollen cellulose (PASC) to glucose. Then, we expressed a highly 4-HBA-resistant chorismate pyruvate-lyase (UbiC) from Providencia rustigianii in the cellulase-displaying strain. This strain produced 975 mg/L of 4-HBA from PASC, which corresponding to 36.8% of the theoretical maximum yield, after 96 h of batch fermentation without the addition of commercial cellulase. This 4-HBA yield was over two times higher than that obtained from glucose (12.3% of the theoretical maximum yield). To our knowledge, this is the first report on the direct production of an aromatic compound from cellulose using cellulase-displaying yeast. 相似文献
979.
Chikage Yoshimizu Kohei Yoshiyama Ichiro Tayasu Tadatoshi Koitabashi Toshi Nagata 《Limnology》2010,11(3):233-239
Detailed cross-sectional temperature and oxygen distributions during winter were examined over 5 years (2003–2007) in large,
warm, monomictic Lake Biwa. The distribution patterns showed year-to-year variation in the degree of spatial heterogeneity
and indicated that both convective mixing and gravity currents contributed to the delivery of oxygen to the profundal zone.
We encountered a warm winter in 2007, when deep-water oxygenation was delayed by more than a month relative to normal years.
Data obtained during the period of oxygenation in 2007 suggest that the timing of complete oxygenation of the bottom water
(or lower layers of the hypolimnion) was controlled by physical processes responsible for destruction of the weak thermal
stratification near the bottom. This study revealed that Lake Biwa is sensitive to year-to-year variations in winter meteorological
conditions. Specifically, the bottom environments are highly susceptible to a warm winter, especially when it follows a cold
winter. 相似文献