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排序方式: 共有499条查询结果,搜索用时 93 毫秒
321.
Hideki Hirakawa Kenta Shirasawa Shunichi Kosugi Kosuke Tashiro Shinobu Nakayama Manabu Yamada Mistuyo Kohara Akiko Watanabe Yoshie Kishida Tsunakazu Fujishiro Hisano Tsuruoka Chiharu Minami Shigemi Sasamoto Midori Kato Keiko Nanri Akiko Komaki Tomohiro Yanagi Qin Guoxin Fumi Maeda Masami Ishikawa Satoru Kuhara Shusei Sato Satoshi Tabata Sachiko N. Isobe 《DNA research》2014,21(2):169-181
Cultivated strawberry (Fragaria x ananassa) is octoploid and shows allogamous behaviour. The present study aims at dissecting this octoploid genome through comparison with its wild relatives, F. iinumae, F. nipponica, F. nubicola, and F. orientalis by de novo whole-genome sequencing on an Illumina and Roche 454 platforms. The total length of the assembled Illumina genome sequences obtained was 698 Mb for F. x ananassa, and ∼200 Mb each for the four wild species. Subsequently, a virtual reference genome termed FANhybrid_r1.2 was constructed by integrating the sequences of the four homoeologous subgenomes of F. x ananassa, from which heterozygous regions in the Roche 454 and Illumina genome sequences were eliminated. The total length of FANhybrid_r1.2 thus created was 173.2 Mb with the N50 length of 5137 bp. The Illumina-assembled genome sequences of F. x ananassa and the four wild species were then mapped onto the reference genome, along with the previously published F. vesca genome sequence to establish the subgenomic structure of F. x ananassa. The strategy adopted in this study has turned out to be successful in dissecting the genome of octoploid F. x ananassa and appears promising when applied to the analysis of other polyploid plant species. 相似文献
322.
Tupaias, or tree shrews, are small mammals that are similar in appearance to squirrels.
The morphological and behavioral characteristics of the group have been extensively
characterized, and despite previously being classified as primates, recent studies have
placed the group in its own family, the Tupaiidae. Genomic analysis has revealed that the
genus Tupaia is closer to humans than it is to rodents. In addition,
tupaias are susceptible to hepatitis B virus and hepatitis C virus. The only other
experimental animal that has been demonstrated to be sensitive to both of these viruses is
the chimpanzee, but restrictions on animal testing have meant that experiments using
chimpanzees have become almost impossible. Consequently, the development of the tupaia for
use as an animal infection model could become a powerful tool for hepatitis virus research
and in preclinical studies on drug development. 相似文献
323.
324.
Shoji Sonoda Jun Yamashita Yozo Koshiyama Yoko Kohara Takashi Enomoto 《Applied Entomology and Zoology》2013,48(1):65-72
A population survey of insects was conducted at peach orchards in Okayama Prefecture, western Japan, every 2 weeks during May–October in 2011. Pitfall traps were used to sample more than 4000 insects at 10 orchards: 8 orchards where ground vegetation had been managed by mowing and 2 with management by herbicide application. Numbers of insect species (species richness) and numbers of insects captured in pitfall traps (trap catches) were greater after mowing. Details of the effects of mowing on insect communities were examined at four orchards that had been mowed. Results suggest that species richness and trap catches increase up to 5 days after mowing and then return to their original state. Increased species richness and trap catches were mainly attributable to the increase of ants (Formicidae) and carabids (Carabidae). These results suggest that ants and carabids actively seek prey animals that have been killed, injured, or damaged by mowing. 相似文献
325.
Yoshinobu Kohara Hideyuki Noda Kazunori Okano Hideki Kambara 《Nucleic acids research》2002,30(16):e87
A DNA analysis platform called ‘Bead-array’ is presented and its features when used in hybridization detection are shown. In ‘Bead-array’, beads of 100-µm diameter are lined in a determined order in a capillary. Each bead is conjugated with DNA probes, and can be identified by its order in the capillary. This probe array is easily produced by just arraying beads conjugated with probes into the capillary in a fixed order. The hybridization is also easily completed by introducing samples (1–300 µl) into the capillary with reciprocal flow. For hybridization detection, as little as 1 amol of fluorescent-labeled oligo DNA was detected. The hybridization reaction was completed in 1 min irrespective of the amount of target DNA. When the number of target molecules was smaller than that of probe molecules on the bead, 10 fmol, almost all targets were captured on the bead. ‘Bead-array’ enables reliable and reproducible measurement of the target quantity. This rapid and sensitive platform seems very promising for various genetic testing tasks. 相似文献
326.
Hatano Jiro Kusama Shoko Tanaka Kenya Kohara Ayaka Miyake Chikahiro Nakanishi Shuji Shimakawa Ginga 《Photosynthesis research》2022,153(1-2):113-120
Photosynthesis Research - Live cyanobacteria and algae integrated onto an extracellular electrode can generate a light-induced current (i.e., a photocurrent). Although the photocurrent is expected... 相似文献
327.
Kramer EL Deutsch GH Sartor MA Hardie WD Ikegami M Korfhagen TR Le Cras TD 《American journal of physiology. Lung cellular and molecular physiology》2007,293(2):L314-L327
Transforming growth factor-alpha (TGF-alpha) and its receptor, the epithelial growth factor receptor (EGFR), have been associated with lung remodeling in premature infants with bronchopulmonary dysplasia (BPD). The goal of this study was to target TGF-alpha overexpression to the saccular phase of lung morphogenesis and determine early alterations in gene expression. Conditional lung-specific TGF-alpha bitransgenic mice and single-transgene control mice were generated. TGF-alpha overexpression was induced by doxycycline (Dox) treatment from embryonic day 16.5 (E16.5) to E18.5. After birth, all bitransgenic pups died by postnatal day 7 (P7). Lung histology at E18.5 and P1 showed abnormal lung morphogenesis in bitransgenic mice, characterized by mesenchymal thickening, vascular remodeling, and poor apposition of capillaries to distal air spaces. Surfactant levels (saturated phosphatidylcholine) were not reduced in bitransgenic mice. Microarray analysis was performed after 1 or 2 days of Dox treatment during the saccular (E17.5, E18.5) and alveolar phases (P4, P5) to identify genes induced by EGFR signaling that were shared or unique to each phase. We found 196 genes to be altered (>1.5-fold change; P < 0.01 for at least 2 time points), with only 32% similarly altered in both saccular and alveolar phases. Western blot analysis and immunostaining showed that five genes selected from the microarrays (egr-1, SP-B, SP-D, S100A4, and pleiotrophin) were also increased at the protein level. Pathological changes in TGF-alpha-overexpressing mice bore similarities to premature infants born in the saccular phase who develop BPD, including remodeling of the distal lung septae and arteries. 相似文献
328.
Ono K Satoh M Yoshida T Ozawa Y Kohara A Takeuchi M Mizusawa H Sawada H 《In vitro cellular & developmental biology. Animal》2007,43(5-6):168-175
We developed a highly sensitive and convenient method of nested polymerase chain reaction (PCR) targeted to mitochondrial
deoxyribonucleic acid (DNA) to identify animal species quickly in cultured cells. Fourteen vertebrate species, including human,
cynomolgus monkey, African green monkey, mouse, rat, Syrian hamster, Chinese hamster, guinea pig, rabbit, dog, cat, cow, pig,
and chicken, could be distinguished from each other by nested PCR. The first PCR amplifies mitochondrial DNA fragments with
a universal primer pair complementary to the conserved regions of 14 species, and the second PCR amplifies the DNA fragments
with species-specific primer pairs from the first products. The species-specific primer pairs were designed to easily distinguish
14 species from each other under standard agarose gel electrophoresis. We further developed the multiplex PCR using a mixture
of seven species-specific primer pairs for two groups of animals. One was comprised of human, mouse, rat, cat, pig, cow, and
rabbit, and the other was comprised of African green monkey, cynomolgus monkey, Syrian hamster, Chinese hamster, guinea pig,
dog, and chicken. The sensitivity of the PCR assay was at least 100 pg DNA/reaction, which was sufficient for the detection
of each species of DNA. Furthermore, the nested PCR method was able to identify the species in the interspecies mixture of
DNA. Thus, the method developed in this study will provide a useful tool for the authentication of animal species. 相似文献
329.
Kubota T Hoshino M Aoki K Ohya K Komano Y Nanki T Miyasaka N Umezawa K 《Arthritis research & therapy》2007,9(5):R97
Inhibition of NF-kappaB is known to be effective in reducing both inflammation and bone destruction in animal models of arthritis. Our previous study demonstrated that a small cell-permeable NF-kappaB inhibitor, dehydroxymethylepoxyquinomicin (DHMEQ), suppresses expression of proinflammatory cytokines and ameliorates mouse arthritis. It remained unclear, however, whether DHMEQ directly affects osteoclast precursor cells to suppress their differentiation to mature osteoclasts in vivo. The effect of DHMEQ on human osteoclastogenesis also remained elusive. In the present study, we therefore examined the effect of DHMEQ on osteoclastogenesis using a mouse collagen-induced arthritis model, and using culture systems of fibroblast-like synovial cells obtained from patients with rheumatoid arthritis, and of osteoclast precursor cells from peripheral blood of healthy volunteers. DHMEQ significantly suppressed formation of osteoclasts in arthritic joints, and also suppressed expression of NFATc1 along the inner surfaces of bone lacunae and the eroded bone surface, while serum levels of soluble receptor activator of NF-kappaB ligand (RANKL), osteoprotegerin and macrophage colony-stimulating factor were not affected by the treatment. DHMEQ also did not suppress spontaneous expression of RANKL nor of macrophage colony-stimulating factor in culture of fibroblast-like synovial cells obtained from patients with rheumatoid arthritis. These results suggest that DHMEQ suppresses osteoclastogenesis in vivo, through downregulation of NFATc1 expression, without significantly affecting expression of upstream molecules of the RANKL/receptor activator of NF-kappaB/osteoprotegerin cascade, at least in our experimental condition. Furthermore, in the presence of RANKL and macrophage colony-stimulating factor, differentiation and activation of human osteoclasts were also suppressed by DHMEQ, suggesting the possibility of future application of NF-kappaB inhibitors to rheumatoid arthritis therapy. 相似文献
330.
Tetsuo Kubota Machiko Hoshino Kazuhiro Aoki Keiichi Ohya Yukiko Komano Toshihiro Nanki Nobuyuki Miyasaka Kazuo Umezawa 《Arthritis research & therapy》2007,9(5):R97
Inhibition of NF-κB is known to be effective in reducing both inflammation and bone destruction in animal models of arthritis.
Our previous study demonstrated that a small cell-permeable NF-κB inhibitor, dehydroxymethylepoxyquinomicin (DHMEQ), suppresses
expression of proinflammatory cytokines and ameliorates mouse arthritis. It remained unclear, however, whether DHMEQ directly
affects osteoclast precursor cells to suppress their differentiation to mature osteoclasts in vivo. The effect of DHMEQ on human osteoclastogenesis also remained elusive. In the present study, we therefore examined the effect
of DHMEQ on osteoclastogenesis using a mouse collagen-induced arthritis model, and using culture systems of fibroblast-like
synovial cells obtained from patients with rheumatoid arthritis, and of osteoclast precursor cells from peripheral blood of
healthy volunteers. DHMEQ significantly suppressed formation of osteoclasts in arthritic joints, and also suppressed expression
of NFATc1 along the inner surfaces of bone lacunae and the eroded bone surface, while serum levels of soluble receptor activator
of NF-κB ligand (RANKL), osteoprotegerin and macrophage colony-stimulating factor were not affected by the treatment. DHMEQ
also did not suppress spontaneous expression of RANKL nor of macrophage colony-stimulating factor in culture of fibroblast-like
synovial cells obtained from patients with rheumatoid arthritis. These results suggest that DHMEQ suppresses osteoclastogenesis
in vivo, through downregulation of NFATc1 expression, without significantly affecting expression of upstream molecules of the RANKL/receptor
activator of NF-κB/osteoprotegerin cascade, at least in our experimental condition. Furthermore, in the presence of RANKL
and macrophage colony-stimulating factor, differentiation and activation of human osteoclasts were also suppressed by DHMEQ,
suggesting the possibility of future application of NF-κB inhibitors to rheumatoid arthritis therapy. 相似文献