全文获取类型
收费全文 | 1756篇 |
免费 | 98篇 |
专业分类
1854篇 |
出版年
2023年 | 3篇 |
2022年 | 13篇 |
2021年 | 28篇 |
2020年 | 5篇 |
2019年 | 24篇 |
2018年 | 18篇 |
2017年 | 14篇 |
2016年 | 33篇 |
2015年 | 52篇 |
2014年 | 78篇 |
2013年 | 83篇 |
2012年 | 122篇 |
2011年 | 110篇 |
2010年 | 64篇 |
2009年 | 56篇 |
2008年 | 135篇 |
2007年 | 108篇 |
2006年 | 102篇 |
2005年 | 103篇 |
2004年 | 128篇 |
2003年 | 119篇 |
2002年 | 130篇 |
2001年 | 24篇 |
2000年 | 14篇 |
1999年 | 20篇 |
1998年 | 26篇 |
1997年 | 15篇 |
1996年 | 13篇 |
1995年 | 17篇 |
1994年 | 15篇 |
1993年 | 15篇 |
1992年 | 16篇 |
1991年 | 14篇 |
1990年 | 11篇 |
1989年 | 15篇 |
1988年 | 16篇 |
1987年 | 9篇 |
1986年 | 4篇 |
1985年 | 9篇 |
1984年 | 6篇 |
1983年 | 13篇 |
1982年 | 13篇 |
1981年 | 7篇 |
1980年 | 7篇 |
1979年 | 7篇 |
1977年 | 4篇 |
1975年 | 3篇 |
1974年 | 3篇 |
1972年 | 2篇 |
1971年 | 2篇 |
排序方式: 共有1854条查询结果,搜索用时 0 毫秒
21.
Kimura Tetsuya; Takeda Shin; Kyozuka Junko; Asahi Tadashi; Shimamoto Ko; Nakamura Kenzo 《Plant & cell physiology》1993,34(2):345-355
A precursor to the 相似文献
22.
23.
Kazuhiko Yoshida Junko Imaki Hidehiko Matsuda † Masatoshi Hagiwara 《Journal of neurochemistry》1995,65(4):1499-1504
Abstract: The signal pathway for light-induced expression of c- fos and the neuropeptide somatostatin (SS) in rat retinal cells was investigated. Flashing light induced c- fos and SS mRNA in the inner nuclear layer and the ganglion cell layer. As both c- fos and SS genes have a cyclic AMP response element (CRE) in their promoters, CRE-binding protein (CREB) phosphorylation in retinal cells was examined with a phospho-CREB-specific antibody. Both flashing light and administration of the L-type Ca2+ channel activator Bay K 8644 induced phosphorylation of CREB in the nuclei of the amacrine cells and the ganglion cells where c- fos /SS mRNAs were expressed. These cells could be double-stained with anti-calmodulin kinase II (anti-CaM kinase II) monoclonal antibody and phospho-CREB-specific polyclonal antiserum after Bay K 8644 administration, indicating the colocalization of phosphorylated CREB at Ser133 and CaM kinase II in the neural retina. 相似文献
24.
Tohru Takata Sun Nyunt Wai Akemi Takade Yoshiro Sawae Junko Ono Kazunobu Amako 《Microbiology and immunology》1995,39(9):639-645
From plate cultures of Campylobacter jejuni grown in room air a particulate protein of 62 kDa was isolated by ion-exchange chromatography. The protein had a square shape from the side view but when viewed from the top it had a star-shaped structure. The molecular size of the whole particle determined by gel filtration was 850 kDa which suggested the presence of 14 subunits of 62 kDa in each particle. The N-terminal 37 amino residues showed more than 80% homology with the sequence of these heat shock protein (HSP) 60 homologs of Chlamydia trachomatis, Helicobacter pylori, and Escherichia coli (GroEL). This protein is immunologically cross-reactive with the antiserum for the 60-kDa HSP of Yersinia enterocolitica. Production of the 62-kDa protein increased under heat stress and growth in an aerobic atmospheric environment. From these observations we concluded that the 62-kDa protein is a Campylobacter stress protein (Cj62) which belongs to the HSP 60 family. 相似文献
25.
26.
Alteration of N-terminal residues of mature human lysozyme affects its secretion in yeast and translocation into canine microsomal vesicles 总被引:2,自引:0,他引:2
Signal sequences play a central role in the initial membrane translocation of secretory proteins. Their functions depend on factors such as hydrophobicity and conformation of the signal sequences themselves. However, some characteristics of mature proteins, especially those of the N-terminal region, might also affect the function of the signal sequences. To examine this possibility, several mutants of human lysozyme modified in the N-terminal region of the mature protein were constructed, and their secretion in yeast as well as in vitro translocation into canine pancreatic microsomes were analyzed using an idealized signal sequence L8 (MR(L)8PLAALG). Our results show the following. (1) Change in the charge at the N-terminal residue of the mature protein does not affect secretion drastically. (2) Substitution of a proline residue at the N terminus prevents cleavage of the signal sequence, although translocation itself is not impaired. (3) Excessive positive charges in the N-terminal region delay translocation of the precursor protein across the membrane. (4) Polar and negatively charged residues introduced into the N-terminal region affect the secretion of the mature protein by preventing its correct folding. 相似文献
27.
Tadao Ohno Xinhai Wang Junko Kurashima Kaoru Saijo-Kurita Masafumi Hirono 《Cytotechnology》1991,7(3):165-172
We have established a novel cell line from a Vero cell derivative that is useful for expression of exogenous genes and protein production. Parental Vero-317 cells can grow in biotin-containing Eagle's MEM without supplements. By transforming this cell line with replication origin-defective SV40 DNA, which contains a temperature-sensitive tsA58 large T antigen gene, we established the Verots S3 cell line that amplified a SV40-origin containing plasmid. The cell line expressed a human growth hormone (hGH) gene insert with higher efficiency than COS-7 cells in 5% serum-containing MEM and could grow and continue hGH expression in protein-free MEM. However, temperature-sensitive shut down of hGH production was observed not immediately but 3 days after the temperature shift from 33°C to 39.5°C. 相似文献
28.
Summary Exocytotic release of the secretory granules of the endocrine cells in the midgut of a cockroach, Periplaneta americana, was studied by means of fixation with tannic acid in combination with glutaraldehyde and osmium tetroxide. A sequence of images indicative of exocytosis suggests the following steps in this process: (1) A delicate connection appears between the granule-limiting membrane and the plasma membrane. (2) The plasma membrane approaches the granule, forming a concave indentation. (3) The granule-limiting membrane fuses with the plasma membrane and opens to give rise to an omega profile. (4) The granule content is voided into extracellular space. Exocytosis occurs not only at the base of the cell but occasionally at its side facing adjacent cells. (5) The exocytotic invagination after release becomes smaller and narrower; sometimes a coated pit with bristles appears. Multiple exocytosis, and exocytosis in the endocrine cells of the nidus, i.e., the regenerative cell mass, are also described. 相似文献
29.
Benichiro Tonomura Hiroshi Nakatani Masatake Ohnishi Junko Yamaguchi-Ito Keitaro Hiromi 《Analytical biochemistry》1978,84(2):370-383
A reaction system suitable for testing the function of a stopped-flow apparatus of high performance was searched for. The reduction of 2,6-dichlorophenol-indophenol by l-ascorbic acid at pH 2.0 was recommended as the most practical test reaction. In due course of the study the reaction mechanism of the reduction was discussed. 相似文献
30.
Removal of the ganglion or severance of the nerve cords at the thorax in mature larvae of the swallowtail, Papilio xuthus, induced systemic distension of the body by swallowing excess air. Such a distension, however, was never induced by simultaneous extirpation of the brain, suboesophageal ganglion, or frontal ganglion, or by severance of the recurrent nerve. Removal of an abdominal ganglion induced distension of the posterior part of the body accompanied by shrinkage of the anterior part. The latter phenomenon appears to be induced by a different mechanism from that of systemic distension. 相似文献