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71.
静磁场对单株人体体表正常菌生长影响的研究 总被引:1,自引:0,他引:1
本文通过40mT和120mT两种静磁场作用下表皮葡萄球菌生长过程的研究,发现试验所选强度静磁场加速了表皮葡萄球菌在对数生长期的生长速率,而在进入稳定生长期后其生长速率反而低于对照组,但就整个生长周期而言,静磁场作用下表皮葡萄球菌的总量大于对照组,表明了试验所选静磁场对表皮葡萄球菌生长有一定促进作用. 相似文献
72.
Ubiquitin receptor proteins play an important role in delivering ubiquitylated protein substrates to the proteasome for degradation. HHR23a and hPLIC2 are two such ubiquitin receptors that contain ubiquitin-like (UBL) domains, which interact with the proteasome, and ubiquitin-associated (UBA) domains, which interact with ubiquitin. Depending on their abundance UBL/UBA family members can either promote or inhibit the degradation of other proteins, which suggests their participation in the delivery of substrates to the proteasome is highly regulated. In previous work, we determined UBL/UBA domain interactions to promote intramolecular interactions in hHR23a that are abrogated with the addition of either ubiquitin or the proteasome component S5a. In yeast, we determined the hHR23a ortholog (Rad23) to interact with another UBL/UBA family member (Ddi1) and to bind a common tetraubiquitin chain. Here, we use NMR spectroscopy to reveal that hHR23a interacts with hPLIC2 via UBL/UBA domain interactions and to map their binding surfaces. In addition, we demonstrate that these two proteins associate in mammalian cells. Intriguingly, inhibition of the proteasome mitigates hHR23a/hPLIC2 interaction. 相似文献
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How the cyclin became a cyclin: regulated proteolysis in the cell cycle. 总被引:48,自引:0,他引:48
75.
Phenol treatment of the glycolipoprotein fromPseudomonas aeruginosa, followed by gel filtration and anion-exchange chromatography, produced a glycolipid fragment free of protein. A second, lipid-rich fragment was obtained as a precipitate of dilute acetic-acid hydrolysis of the glycolipoprotein. After solubilization, the lipid-rich fragment was processed on Sephadex G-200. Both lipid-containing fragments were immunologically homogeneous in Ouchterlony plates against rabbit antiserum to crude glycolipoprotein. The precipitin bands indicated that polysaccharide residues conferred the antigenic specificity. General chemical analyses were made on each fragment. All lipid-containing fragments were lethal, to varying degrees, on injection in mice. Proteinase K digestion of the glycolipoprotein did not reduce its lethality. All lipid-containg fragments stimulated varying levels of antibody capable of passively protecting mice against lethal challenge with viablePseudomonas aeruginosa. The contribution of the carbohydrate moiety to the expression of lethality is discussed. 相似文献
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Jeniffer Farias dos Santos Emily Marques dos Reis Fernanda Vieira Berti Guilherme Colla Janice Koepp Viviane Abreu Nunes 《Biotechnology and bioengineering》2023,120(12):3602-3611
Currently, there is a great need for the development of three-dimensional (3D) in vitro lung models. Particularly, the production of a suitable 3D model of pulmonary epithelium for understanding the pathophysiology of diseases such as the COVID-19 must consider the tissue architecture and presence, for example, of the angiotensin-converting enzyme-2 (ACE-2) in the cells. Different polymeric membranes are being used to support cell culturing, especially of lung cells, however, there is still no information about the culture of these cells onto bacterial nanocellulose (BNC) matrices. We have used the BNC matrix CellFate® as a support for the assembly of a 3D in vitro model of lung epithelium, composed of human lung fibroblasts (HLF) and lung adenocarcinoma cells (CALU-3). CellFate® matrices were made from bacterial fermentation resulting in a natural and biocompatible biopolymer. Cells were cultured onto CellFate® and maintained in a 5% CO2 humidified atmosphere at 37°C. Cell viability was assessed by the resazurin method The samples were, then, exposed to the air–liquid interface (ALI), and histologically analyzed. ACE-2 activity was verified on the hydrolyze of the fluorogenic substrate Mca-APK(Dnp)-OH, and its presence was evaluated by flow cytometry. The expression of the anionic transporter SLCO3A1 was evaluated by qPCR. Cell viability analysis indicates that CellFate® was not toxic to these cells. By flow cytometry, the presence of the ACE-2 was identified in the CALU-3 cells surface corroborating the results obtained from enzymatic activity analysis. The SLCO3A1 transporter expression was identified in cells cultured onto CellFate®, but not in cells cultured onto the transwell (control). CALU-3 cells cultivated onto CellFate® resulted in a pseudostratified organization, a typical morphology of the human respiratory tract epithelium. The current model opens perspectives for studies involving physiological characterization, improving its relevance for the understanding of the pathophysiology of diseases as well as the response to drugs. 相似文献