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171.
Phoebe Barnes Brian R. Wilson Nick Reid Terry B. Koen Peter Lockwood David W. Lamb 《Plant and Soil》2011,345(1-2):339-352
Scattered paddock trees are a keystone feature of temperate grazing landscapes of Australia. However, our understanding of their influence on their immediate environment, and specifically the spatial distribution and characteristics of litter, is still limited. Here, we quantified the spatial pattern of litter around 4 Eucalyptus species (Eucalyptus melliodora A. Cunn. Ex Schauer, E. viminalis Labill., E. blakelyi Maiden and E. michaeliana Blakely) in grazing landscapes on the Northern Tablelands of NSW, Australia. We examined the effect of species and soil parent material (basalt, granite and meta-sediments) on litter chemistry and chemical pools. Between 54–145 kg of litter was found around individual trees and litter density consistently declined with distance from the tree (330 g.m?2 in the inner canopy to 4 g.m?2 in the open paddock). However, an equivalent quantity of litter was found beneath and beyond the canopy indicating that a large quantity of the litter and nutrients fell beyond the edge of the canopy. Overall, leaf litter accounted for 23 to 34% of litterfall and had larger nutrient concentrations and pools than bark or stick litter. Most litter nutrients concentrations were independent of tree species or parent material but our results suggest that P, K and S were removed in foliage prior to abscission whilst Ca and Fe concentrations increased. The spatial patterns of litter distribution around scattered trees coincide with spatial patterns in soil properties that are frequently observed in these environments, and provide strong evidence of a significant link between these factors. Our results suggest that the removal of scattered trees from pastoral landscapes in this region of Australia will result in the loss of a significant litter input to the soil surface and will diminish this potentially important source of soil nutrients. 相似文献
172.
In recent years, plants have been developed as an alternative expression system to mammalian hosts for the production of therapeutic proteins. Many modifications to the plant glycosylation machinery have been made to render it more human because of the importance of glycosylation for functionality, serum half-life, and the safety profile of the expressed proteins. These modifications include removal of plant-specific β1,2-xylose and core α1,3-fucose, and addition of bisecting N-acetylglucosamine, β1,4-galactoses, and sialic acid residues. Another glycosylation step that is essential for the production of complex human-type glycans is the synthesis of multiantennary structures, which are frequently found on human N-glycans but are not generated by wild-type plants. Here, we report both the magnICON-based transient as well as stable introduction of the α1,3-mannosyl-β1,4-N-acetylglucosaminyltransferase (GnT-IV isozymes a and b) and α1,6-mannosyl-β1,6-N-acetylglucosaminyltransferase (GnT-V) in Nicotiana benthamiana plants. The enzymes were targeted to the Golgi apparatus by fusing their catalytic domains to the plant-specific localization signals of xylosyltransferase and fucosyltransferase. The GnT-IV and -V modifications were tested in the wild-type background, but were also combined with the RNA interference-mediated knockdown of β1,2-xylosyltransferase and α1,3-fucosyltransferase. Results showed that triantennary Gn[GnGn] and [GnGn]Gn N-glycans could be produced according to the expected activities of the respective enzymes. Combination of the two enzymes by crossing stably transformed GnT-IV and GnT-V plants showed that up to 10% tetraantennary [GnGn][GnGn], 25% triantennary, and 35% biantennary N-glycans were synthesized. All transgenic plants were viable and showed no aberrant phenotype under standard growth conditions. 相似文献
173.
Wang P Kopittke PM De Schamphelaere KA Zhao FJ Zhou DM Lock K Ma YB Peijnenburg WJ McGrath SP 《The New phytologist》2011,192(2):414-427
Assessing environmental risks of metal contamination in soils is a complex task because the biologically effective concentrations of metals in soils vary widely with soil properties. The factors influencing the toxic effect of nickel (Ni) on root growth of barley (Hordeum vulgare) were re-evaluated using published data from both soil and hydroponic cultures. The electrical potential (ψ(0) (o) ) and ion activities ({I(z) }(0) (o) ) at the outer surfaces of root-cell plasma membranes (PMs) were computed as the basis of the re-evaluation. The reanalyses demonstrated that root growth was related to: the Ni(2+) activity at the PM surface, ({Ni(2+) }(0) (o) ); calcium (Ca) deficiency (related to {Ca(2+) }(0) (o) ); osmotic effects; and modification of intrinsic Ni(2+) toxicity by magnesium (Mg(2+) ; this appeared to exert an intrinsic (specific) ameliorating effect on intrinsic Ni(2+) toxicity). Electrostatic toxicity models (ETM) were developed to relate root growth to these factors (R(2) > 0.751). Based on the ETM developed in soil culture and a Ni(2+) solid-solution partitioning model, critical metal concentrations in soils linked to a biological effect were well predicted for 16 European soils with a wide range of properties, indicating the potential utility of ETM in risk assessment of metals in terrestrial ecosystems. 相似文献
174.
Verhees KJ Schols AM Kelders MC Op den Kamp CM van der Velden JL Langen RC 《American journal of physiology. Cell physiology》2011,301(5):C995-C1007
Skeletal muscle atrophy commonly occurs in acute and chronic disease. The expression of the muscle-specific E3 ligases atrogin-1 (MAFbx) and muscle RING finger 1 (MuRF1) is induced by atrophy stimuli such as glucocorticoids or absence of IGF-I/insulin and subsequent Akt signaling. We investigated whether glycogen synthase kinase-3β (GSK-3β), a downstream molecule in IGF-I/Akt signaling, is required for basal and atrophy stimulus-induced expression of atrogin-1 and MuRF1, and myofibrillar protein loss in C(2)C(12) skeletal myotubes. Abrogation of basal IGF-I signaling, using LY294002, resulted in a prominent induction of atrogin-1 and MuRF1 mRNA and was accompanied by a loss of myosin heavy chain fast (MyHC-f) and myosin light chains 1 (MyLC-1) and -3 (MyLC-3). The synthetic glucocorticoid dexamethasone (Dex) also induced the expression of both atrogenes and likewise resulted in the loss of myosin protein abundance. Genetic ablation of GSK-3β using small interfering RNA resulted in specific sparing of MyHC-f, MyLC-1, and MyLC-3 protein levels after Dex treatment or impaired IGF-I/Akt signaling. Interestingly, loss of endogenous GSK-3β suppressed both basal and atrophy stimulus-induced atrogin-1 and MuRF1 expression, whereas pharmacological GSK-3β inhibition, using CHIR99021 or LiCl, only reduced atrogin-1 mRNA levels in response to LY294002 or Dex. In conclusion, our data reveal that myotube atrophy and myofibrillar protein loss are GSK-3β dependent, and demonstrate for the first time that basal and atrophy stimulus-induced atrogin-1 mRNA expression requires GSK-3β enzymatic activity, whereas MuRF1 expression depends solely on the physical presence of GSK-3β. 相似文献
175.
Galactose-modified iNKT cell agonists stabilized by an induced fit of CD1d prevent tumour metastasis
Aspeslagh S Li Y Yu ED Pauwels N Trappeniers M Girardi E Decruy T Van Beneden K Venken K Drennan M Leybaert L Wang J Franck RW Van Calenbergh S Zajonc DM Elewaut D 《The EMBO journal》2011,30(11):2294-2305
Invariant natural killer T (iNKT) cells are known to have marked immunomodulatory capacity due to their ability to produce copious amounts of effector cytokines. Here, we report the structure and function of a novel class of aromatic α-galactosylceramide structurally related glycolipids with marked Th1 bias in both mice and men, leading to superior tumour protection in vivo. The strength of the Th1 response correlates well with enhanced lipid binding to CD1d as a result of an induced fit mechanism that binds the aromatic substitution as a third anchor, in addition to the two lipid chains. This induced fit is in contrast to another Th1-biasing glycolipid, α-C-GalCer, whose CD1d binding follows a conventional key-lock principle. These findings highlight the previously unexploited flexibility of CD1d in accommodating galactose-modified glycolipids and broaden the range of glycolipids that can stimulate iNKT cells. We speculate that glycolipids can be designed that induce a similar fit, thereby leading to superior and more sustained iNKT cell responses in vivo. 相似文献
176.
Koen Vandelannoote Kurt Jordaens Pieter Bomans Herwig Leirs Lies Durnez Dissou Affolabi Ghislain Sopoh Julia Aguiar Delphin Mavinga Phanzu Kapay Kibadi Sara Eyangoh Louis Bayonne Manou Richard Odame Phillips Ohene Adjei Anthony Ablordey Leen Rigouts Fran?oise Portaels Miriam Eddyani Bouke C. de Jong 《Applied and environmental microbiology》2014,80(3):1197-1209
Buruli ulcer is an indolent, slowly progressing necrotizing disease of the skin caused by infection with Mycobacterium ulcerans. In the present study, we applied a redesigned technique to a vast panel of M. ulcerans disease isolates and clinical samples originating from multiple African disease foci in order to (i) gain fundamental insights into the population structure and evolutionary history of the pathogen and (ii) disentangle the phylogeographic relationships within the genetically conserved cluster of African M. ulcerans. Our analyses identified 23 different African insertion sequence element single nucleotide polymorphism (ISE-SNP) types that dominate in different areas where Buruli ulcer is endemic. These ISE-SNP types appear to be the initial stages of clonal diversification from a common, possibly ancestral ISE-SNP type. ISE-SNP types were found unevenly distributed over the greater West African hydrological drainage basins. Our findings suggest that geographical barriers bordering the basins to some extent prevented bacterial gene flow between basins and that this resulted in independent focal transmission clusters associated with the hydrological drainage areas. Different phylogenetic methods yielded two well-supported sister clades within the African ISE-SNP types. The ISE-SNP types from the “pan-African clade” were found to be widespread throughout Africa, while the ISE-SNP types of the “Gabonese/Cameroonian clade” were much rarer and found in a more restricted area, which suggested that the latter clade evolved more recently. Additionally, the Gabonese/Cameroonian clade was found to form a strongly supported monophyletic group with Papua New Guinean ISE-SNP type 8, which is unrelated to other Southeast Asian ISE-SNP types. 相似文献
177.
Shifts in species distributions due to environmental change may affect the spatial pattern of genetic structure within a species' range, including possible changes to the adaptive potential of populations. We investigated spatial patterns of neutral genetic diversity and differentiation at the southern edge of the Canada lynx Lynx canadensis distribution in Ontario, Canada. We analyzed provincial fur harvest records (1972–2010) and collected and genotyped lynx pelt samples (2007–2009) from 702 lynx at 14 microsatellite loci. We show that the southern range boundary of lynx in central Canada has contracted northward by > 175 km since the 1970s, and that high winter temperature, low snow depth, and low proportion of suitable habitat are strongly correlated with low neutral genetic diversity and high genetic differentiation at the trailing range edge. Our work tests fundamental ideas about species range limits and demonstrates that environmental conditions can have a marked influence on neutral genetic structure. Our results suggest that changes in environmental conditions will result in further loss of genetic diversity and possibly reduce adaptive potential in southern peripheral lynx populations. 相似文献
178.
Comparing the Device Physics and Morphology of Polymer Solar Cells Employing Fullerenes and Non‐Fullerene Acceptors
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Jason T. Bloking Tommaso Giovenzana Andrew T. Higgs Andrew J. Ponec Eric T. Hoke Koen Vandewal Sangwon Ko Zhenan Bao Alan Sellinger Michael D. McGehee 《Liver Transplantation》2014,4(12)
There is a need to find electron acceptors for organic photovoltaics that are not based on fullerene derivatives since fullerenes have a small band gap that limits the open‐circuit voltage (VOC), do not absorb strongly and are expensive. Here, a phenylimide‐based acceptor molecule, 4,7‐bis(4‐(N‐hexyl‐phthalimide)vinyl)benzo[c]1,2,5‐thiadiazole (HPI‐BT), that can be used to make solar cells with VOC values up to 1.11 V and power conversion efficiencies up to 3.7% with two thiophene polymers is demonstrated. An internal quantum efficiency of 56%, compared to 75–90% for polymer‐fullerene devices, results from less efficient separation of geminate charge pairs. While favorable energetic offsets in the polymer‐fullerene devices due to the formation of a disordered mixed phase are thought to improve charge separation, the low miscibility (<5 wt%) of HPI‐BT in polymers is hypothesized to prevent the mixed phase and energetic offsets from forming, thus reducing the driving force for charges to separate into the pure donor and acceptor phases where they can be collected. 相似文献
179.
Ronald C. Kessler Sherri Rose Karestan C. Koenen Elie G. Karam Paul E. Stang Dan J. Stein Steven G. Heeringa Eric D. Hill Israel Liberzon Katie A. McLaughlin Samuel A. McLean Beth E. Pennell Maria Petukhova Anthony J. Rosellini Ayelet M. Ruscio Victoria Shahly Arieh Y. Shalev Derrick Silove Alan M. Zaslavsky Matthias C. Angermeyer Evelyn J. Bromet José Miguel Caldas de Almeida Giovanni de Girolamo Peter de Jonge Koen Demyttenaere Silvia E. Florescu Oye Gureje Josep Maria Haro Hristo Hinkov Norito Kawakami Viviane Kovess‐Masfety Sing Lee Maria Elena Medina‐Mora Samuel D. Murphy Fernando Navarro‐Mateu Marina Piazza Jose Posada‐Villa Kate Scott Yolanda Torres Maria Carmen Viana 《World psychiatry》2014,13(3):265-274
Post‐traumatic stress disorder (PTSD) should be one of the most preventable mental disorders, since many people exposed to traumatic experiences (TEs) could be targeted in first response settings in the immediate aftermath of exposure for preventive intervention. However, these interventions are costly and the proportion of TE‐exposed people who develop PTSD is small. To be cost‐effective, risk prediction rules are needed to target high‐risk people in the immediate aftermath of a TE. Although a number of studies have been carried out to examine prospective predictors of PTSD among people recently exposed to TEs, most were either small or focused on a narrow sample, making it unclear how well PTSD can be predicted in the total population of people exposed to TEs. The current report investigates this issue in a large sample based on the World Health Organization (WHO)'s World Mental Health Surveys. Retrospective reports were obtained on the predictors of PTSD associated with 47,466 TE exposures in representative community surveys carried out in 24 countries. Machine learning methods (random forests, penalized regression, super learner) were used to develop a model predicting PTSD from information about TE type, socio‐demographics, and prior histories of cumulative TE exposure and DSM‐IV disorders. DSM‐IV PTSD prevalence was 4.0% across the 47,466 TE exposures. 95.6% of these PTSD cases were associated with the 10.0% of exposures (i.e., 4,747) classified by machine learning algorithm as having highest predicted PTSD risk. The 47,466 exposures were divided into 20 ventiles (20 groups of equal size) ranked by predicted PTSD risk. PTSD occurred after 56.3% of the TEs in the highest‐risk ventile, 20.0% of the TEs in the second highest ventile, and 0.0‐1.3% of the TEs in the 18 remaining ventiles. These patterns of differential risk were quite stable across demographic‐geographic sub‐samples. These results demonstrate that a sensitive risk algorithm can be created using data collected in the immediate aftermath of TE exposure to target people at highest risk of PTSD. However, validation of the algorithm is needed in prospective samples, and additional work is warranted to refine the algorithm both in terms of determining a minimum required predictor set and developing a practical administration and scoring protocol that can be used in routine clinical practice. 相似文献
180.