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101.
Koen B. Tanghe Alexis De Tiège Lieven Pauwels Stefaan Blancke Johan Braeckman 《Biology & philosophy》2018,33(3-4):23
Welch (Biol Philos 32(2):263–279, 2017) has recently proposed two possible explanations for why the field of evolutionary biology is plagued by a steady stream of claims that it needs urgent reform. It is either seriously deficient and incapable of incorporating ideas that are new, relevant and plausible or it is not seriously deficient at all but is prone to attracting discontent and to the championing of ideas that are not very relevant, plausible and/or not really new. He argues for the second explanation. This paper presents a twofold critique of his analysis: firstly, the main calls for reform do not concern the field of evolutionary biology in general but rather, or more specifically, the modern evolutionary synthesis. Secondly, and most importantly, these calls are not only inspired by the factors, enumerated by Welch, but are also, and even primarily, motivated by four problematic characteristics of the modern synthesis. This point is illustrated through a short analysis of the latest reform challenge to the modern synthesis, the so-called extended evolutionary synthesis. We conclude with the suggestion that the modern synthesis should be amended, rather than replaced. 相似文献
102.
Isolation and characterization of lectins and lectin-alliinase complexes from bulbs of garlic (Allium sativum) and ramsons (Allium ursinum) 总被引:1,自引:0,他引:1
Koen Smeets Els J.M Van Damme Fred Van Leuven Willy J Peumans 《Glycoconjugate journal》1997,14(3):331-343
A procedure developed to separate the homodimeric and heterodimeric mannose-binding lectins from bulbs of garlic (Allium sativum
L.) and ramsons (Allium ursinum L.) also enabled the isolation of stable lectin-alliinase complexes. Characterization of the
individual lectins indicated that, in spite of their different molecular structure, the homomeric and heteromeric lectins
resemble each other reasonably well with respect to their agglutination properties and carbohydrate-binding specificity. However,
a detailed analysis of the lectin-alliinase complexes from garlic and ramsons bulbs demonstrated that only the heterodimeric
lectins are capable of binding to the glycan chains of the alliinase molecules (EC 4.4.1.4). Moreover, it appears that only
a subpopulation of the alliinase molecules is involved in the formation of lectin-alliinase complexes and that the complexed
alliinase contains more glycan chains than the free enzyme. Finally, some arguments are given that the lectin-alliinase complexes
do not occur in vivo but are formed in vitro after homogenization of the tissue.
This revised version was published online in November 2006 with corrections to the Cover Date. 相似文献
103.
Isolation,characterization and molecular cloning of the mannose-binding lectins from leaves and roots of garlic (Allium sativum L.) 总被引:4,自引:0,他引:4
Smeets Koen Van Damme Els J.M. Verhaert Peter Barre Annick Rougé Pierre Van Leuven Fred Peumans Willy J. 《Plant molecular biology》1997,33(2):223-234
Two novel lectins were isolated from roots and leaves of garlic. Characterization of the purified proteins indicated that the leaf lectin ASAL is a dimer of two identical subunits of 12 kDa, which closely resembles the leaf lectins from onion, leek and shallot with respect to its molecular structure and agglutination activity. In contrast, the root lectin ASARI, which is a dimer of subunits of 15 kDa, strongly differs from the leaf lectin with respect to its agglutination activity. cDNA cloning of the leaf and root lectins revealed that the deduced amino acid sequences of ASAL and ASARI are virtually identical. Since both lectins have identical N-terminal sequences the larger Mr of the ASARI subunits implies that the root lectin has an extra sequence at its C-terminus. These results not only demonstrate that virtually identical precursor polypeptides are differently processed at their C-terminus in roots and leaves but also indicate that differential processing yields mature lectins with strongly different biological activities. Further screening of the cDNA library for garlic roots also yielded a cDNA clone encoding a protein composed of two tandemly arrayed lectin domains. Since the presumed two-domain root lectin has not been isolated yet, its possible relationship to the previously described two-domain bulb lectin could not be studied at the protein level. 相似文献
104.
Koen Uytterhoeven Jiri Sponer Luc Van Meervelt 《European journal of biochemistry》2002,269(12):2868-2877
Single-crystal X-ray structure determinations of the complex between the minor-groove binder distamycin and d(GGCCAATTGG) reveal two 1 : 1 binding modes which differ in the orientation of the drug molecule in the minor groove. The two crystals were grown from different crystallization conditions and found to diffract to 2.38 and 1.85 A, respectively. The structures were refined to completion using SHELXL-93, resulting in a residual R factor of 20.30% for the 2.38-A resolution structure (including 46 water molecules) and 19.74% for the 1.85-A resolution structure (including 74 water molecules). In both orientations, bifurcated hydrogen bonds are formed between the amide nitrogen atoms of the drug and AT base pairs. With a binding site of at least five base pairs, close contacts between the terminal distamycin atoms and guanine amino groups are inevitable. The detailed nature of several of these interactions was further investigated by ab initio quantum chemical methods. 相似文献
105.
Anton L. van Helden Alan N. Baker Merel L. Dalebout Julio C. Reyes Koen Van Waerebeek C. Scott Baker 《Marine Mammal Science》2002,18(3):609-621
Mesoplodon traversii (Gray, 1874) is shown to be a senior synonym of the recently described beaked whale Mesoplodon hahamondi Reyes et al. , 1995 on the basis of a phylogenetic analysis of mitochondrial DNA control region sequences. The mandible and teeth of M. traversii , first reported in 1873 by Hector as Dolichodon layardii . are redescribed. The species can be distinguished by features of the calvaria; including the large jugal, broad rostrum, and small distance between premaxillary foramina. The male teeth, which are large and spade-shaped with a strong terminal denticle, are also diagnostic. M. traversii is known only from Pitt Island and White Island, New Zealand and Robinson Crusoe Island, Juan Fernandez Archipelago, Chile. 相似文献
106.
107.
108.
Paul M. Sanders Anhthu Q. Bui Koen Weterings K. N. McIntire Yung-Chao Hsu Pei Yun Lee Mai Thy Truong T. P. Beals R. B. Goldberg 《Sexual plant reproduction》1999,11(6):297-322
We identified Arabidopsis thaliana sterility mutants by screening T-DNA and EMS-mutagenized lines and characterized several male-sterile mutants with defects
specific for different anther processes. Approximately 44 and 855 sterile mutants were uncovered from the T-DNA and EMS screens,
respectively. Several mutants were studied in detail with defects that included the establishment of anther morphology, microspore
production, pollen differentiation, and anther dehiscence. Both non-dehiscencing and late-dehiscencing mutants were identified.
In addition, pollenless mutants were observed with either apparent meiotic defects and/or abnormalities in cell layers surrounding
the locules. Two mutant alleles were identified for the POLLENLESS3 locus which have defects in functional microspore production that lead to the degeneration of cells within the anther locules.
pollenless3–1 contains a T-DNA insertion that co-segregates with the mutant phenotype and pollenless3–2 has a large deletion in the POLLENLESS3 gene. The POLLENLESS3 gene has no known counterparts in the GenBank, but encodes a protein containing putative nuclear localization and protein-protein
interaction motifs. The POLLENLESS3 gene was shown recently to be the same as MS5, a previously described Arabidopsis
thaliana male-sterility mutant. Three genes were identified in the POLLENLESS3 genomic region: GENEY, POLLENLESS3, and β9-TUBULIN. The segment of the Arabidopsis
thaliana genome containing the POLLENLESS3 and β9-TUBULIN genes is duplicated and present on a different chromosome. Analysis of the POLLENLESS3 expression pattern determined that the 1.3-kb POLLENLESS3 mRNA is localized specifically within meiotic cells in the anther
locules and that POLLENLESS3 mRNA is present only during late meiosis.
Received: 15 October 1998 / Revision accepted: 19 November 1998 相似文献
109.
Marcel André Fischer Koen Temmerman Ebru Ercan Walter Nickel Matthias Seedorf 《Traffic (Copenhagen, Denmark)》2009,10(8):1084-1097
Recruitment of cytosolic proteins to individual membranes is governed by a combination of protein–protein and protein–membrane interactions. Many proteins recognize phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2 ] at the cytosolic surface of the plasma membrane (PM). Here, we show that a protein–lipid interaction can also serve as a dominant signal for the sorting of integral membrane proteins. Interaction with phosphatidly-inositolphosphates (PIPs) at the PM is involved in the targeting of the polytopic yeast protein Ist2 to PM-associated domains of the cortical endoplasmic reticulum (ER). Moreover, binding of PI(4,5)P2 at the PM functions as a dominant mechanism that targets other integral membrane proteins to PM-associated domains of the cortical ER. This sorting to a subdomain of the ER abolishes proteasomal degradation and trafficking along the classical secretory (sec) pathway. In combination with the localization of IST2 mRNA to the bud tip and other redundant signals in Ist2, binding of PIPs leads to efficient accumulation of Ist2 at domains of the cortical ER from where the protein may reach the PM independently of the function of the sec-pathway. 相似文献
110.
Boundaries of the wolf and the wild: a conceptual examination of the relationship between rewilding and animal reintroduction 总被引:1,自引:0,他引:1 下载免费PDF全文
Animal reintroduction and rewilding are two widely appealing and frequently connected forms of ecological restoration. However, the critical assumption that animal reintroduction automatically helps to restore formerly wild places is under‐theorized. To fill this void, we identified three common rewilding elements from the literature—ecological functioning, wilderness experience, and natural autonomy—and screened these against a hypothetical wolf reintroduction to Scotland. Each of the rewilding elements was likely to be positively impacted by a wolf reintroduction. Yet, there is a key conceptual difficulty in that the different rewilding elements do not necessarily enforce each other, and at times may even collide. Thus, a reintroduced species like the wolf may obfuscate the clear‐cut, purified nature category to which rewilding often aspires. As a way forward, we suggest that there is merit in actively engaging with the tensions created by rewilding and reintroductions. A reconceptualisation of the nature–culture spectrum as consisting of multiple layers (e.g. ecological functioning, wilderness experience, and natural autonomy) may help to interpret ecological restoration as a tentative, deliberative, and gradual enterprise. This bears some resemblance to the notion of approaching a landscape like a ‘palimpsest’ (i.e. a text built up of different layers written on top of each other), which may support the reconciliation of conflicting views without necessarily making those disappear. When viewed as feeding into a multilayered nature–culture spectrum, animal reintroduction and rewilding can be promoted as inspiring and essentially non‐controlling forms of ecological restoration and human interaction with nature. 相似文献