Structure of major complex carbohydrate chains of influenza virus A/Leningrad/385/80 (H3N2) hemagglutinin

The structure of four oligosaccharides which are the main carbohydrate chains of hemagglutinin of influenza virus A/Leningrad/385/80 (H3N2) has been elucidated. It was shown by means of enzymatic and mild acid hydrolysis, Smith degradation and acetolysis that the oligosaccharides have very similar structures (noncomplete triantennary) and differ from each other only in the number (0, 1 or 2) and position of fucose residues. The peculiarities of glycosylation of H3 hemagglutinin from different strains of influenza virus were discussed.     

A carbohydrate-containing copolymer with the specificity of a capsular polysaccharide of type 3 Streptococcus pneumoniae

The synthesis of allyl beta-glycoside of cellobiuronic acid by chemical modification of cellobiose was described. The carbohydrate-containing polymers with different content of determinant groups were obtained via radical copolymerization of this hapten with acrylamide. The copolymer which contained 27% carbohydrates and had molecular mass about 100-300 kilodaltons had the serological specificity of the capsular polysaccharide Streptococcus pneumoniae type 3 as shown by an enzyme linked immunosorbent assay.     

Conformation analysis of carbohydrate chains of group-specific substances

Theoretical conformational analysis of bi- and three-antennary carbohydrate chains of H-specific group substances has been carried out. It has been shown that O-glycosylating oligosaccharides can form compact Y-shaped structures with effective non-bonded interactions between the antennae residues.     

Antigenic polysaccharides of bacteria. 37. Structure of the polysaccharide chain of Pseudomonas syringae pv.tabaci (serogroup VII) lipopolysaccharide]

The structure of the O-specific polysaccharide chain of Pseudomonas syringae pv. tabaci strain 223 (serogroup VII) lipopolysaccharide was established on the basis of one- and two-dimensional 1H NMR analysis, 13C NMR analysis and calculation of optical rotation. The structure determined by the non-destructive way was confirmed by acid hydrolysis and methylation. (Sequence: see text). O-Antigen of the strain studied is similar in structure and serological properties to O-antigens of Pseudomonas syringae strains belonging to serogroup I.     

Chemico-enzymatic synthesis of O-specific polysaccharide of Salmonella anatum and its analogs using partially purified polymerase preparation

A partial purified polymerase from S. anatum was used for the synthesis of polysaccharide [-6) [14C]Man(beta 1-4)Rha(alpha 1-3)Gal(alpha 1-]n and its analogues containing D-glucose residue instead of D-galactose or D-mannose. Structures of these polymers were confirmed by methylation analysis and radioimmunochemical tests.     

Structure of Proteus mirabilis O27 O-specific polysaccharide containing amino acids and phosphoethanolamine

The following structure of the repeating unit of the O-specific polysaccharide of Proteus mirabilis O27, containing amides of D-glucuronic and D-galacturonic acids with L-lysine and L-alanine, respectively, N-acetyl-D-glucosamine and phosphoethanolamine, has been determined: (sequence; see text) The main methods used for structural analysis of the polysaccharide were selective solvolysis with anhydrous hydrogen fluoride and Smith degradation, as well as NMR spectroscopy and mass spectrometry. The immunodominant role of the lateral N-acetyl-D-glucosamine and phosphoethanolamine in manifesting the serological specificity of P. mirabilis O27 has been established.