全文获取类型
收费全文 | 1030篇 |
免费 | 175篇 |
出版年
2019年 | 12篇 |
2016年 | 17篇 |
2015年 | 31篇 |
2014年 | 33篇 |
2013年 | 32篇 |
2012年 | 28篇 |
2011年 | 44篇 |
2010年 | 21篇 |
2009年 | 24篇 |
2008年 | 40篇 |
2007年 | 33篇 |
2006年 | 23篇 |
2005年 | 30篇 |
2004年 | 19篇 |
2003年 | 18篇 |
2002年 | 21篇 |
2001年 | 28篇 |
2000年 | 22篇 |
1999年 | 26篇 |
1998年 | 19篇 |
1997年 | 12篇 |
1996年 | 14篇 |
1995年 | 15篇 |
1994年 | 15篇 |
1993年 | 12篇 |
1992年 | 21篇 |
1991年 | 29篇 |
1990年 | 37篇 |
1989年 | 42篇 |
1988年 | 28篇 |
1987年 | 28篇 |
1986年 | 20篇 |
1985年 | 23篇 |
1984年 | 36篇 |
1983年 | 20篇 |
1982年 | 18篇 |
1981年 | 20篇 |
1980年 | 16篇 |
1979年 | 22篇 |
1978年 | 22篇 |
1977年 | 16篇 |
1976年 | 21篇 |
1975年 | 23篇 |
1974年 | 19篇 |
1972年 | 14篇 |
1971年 | 13篇 |
1970年 | 11篇 |
1969年 | 14篇 |
1968年 | 11篇 |
1967年 | 10篇 |
排序方式: 共有1205条查询结果,搜索用时 15 毫秒
21.
In the plasma membranes from several mammalian tissues (including normal and tumor tissues), a Mg2+ (or Ca2+)-dependent ATP phosphohydrolase activity is present in much greater amount than the (Na+ + K+)-ATPase. The ouabain-insensitive activity can be attributed to at least two enzymes, an ATPase (EC 3.6.1.3) and an ATP diphosphohydrolase (EC 3.6.1.5). The ATPase hydrolyzes ATP and other nucleoside triphosphates and is not inhibited by azide. The ATP diphosphohydrolase hydrolyzes both ATP and ADP (and other nucleoside tri- and diphosphates) and the hydrolysis of adenine nucleotides is strongly inhibited by 10 mM azide. The ratios of these two enzymes in the various membranes (as determined by the extent of azide inhibition) vary widely. The ATP diphosphohydrolase accounts for most of the Mg2+ (or Ca2+)-dependent ATP hydrolysis activity of the plasma membranes of liver (mouse), kidney (dog), two mouse sarcomas, and a human astrocytoma (xenograft in athymic mice). The ATPase is more dominant in the plasma membranes from mouse brain and human oat cell carcinoma. The widespread presence of the ATP diphosphohydrolase in plasma membrane from various types of tissues is demonstrated for the first time and is of particular interest in view of its relatively high activity in the plasma membranes of two sarcomas. The membrane-bound ATP diphosphohydrolase is characterized with respect to its metal ion activators, substrates, and inhibitors. These results should facilitate the distinction of this enzyme from other ATP hydrolyzing enzymes of plasma membranes in future investigations. 相似文献
22.
Isolation and growth of a Pseudomonas species that utilizes cyanide as a source of nitrogen 总被引:3,自引:0,他引:3
A simple method of isolating bacteria that utilize cyanide as a source of nitrogen for growth has been developed. This involved supplying hydrogen cyanide as a vapour to glucose-containing minimal-salts agar plates. The bacteria isolated were Gram-negative, oxidase-positive rods producing a fluorescent green pigment and were tentatively identified as strains of Pseudomonas fluorescens. Three organisms were studied further and shown to be P. fluorescens biotype II. One of these (NCIB 11764) was grown in a glucose-containing fed-batch culture with either NH4Cl or KCN as the limiting nutrient. Cyanide-grown bacteria produced stoichiometric amounts of ammonia from cyanide when pulsed with cyanide under aerobic conditions. Stimulation of oxygen uptake was seen on addition of cyanide to suspensions of cyanide-grown but not ammonia-grown bacteria. 相似文献
23.
M van de Rijn P G Lerch R W Knowles C Terhorst 《Journal of immunology (Baltimore, Md. : 1950)》1983,131(2):851-855
The human beta 2-microglobulin (beta-2m)-associated human thymocyte differentiation antigens T6 and M241 were compared using biochemical techniques. T6 and M241 antigens reside on different molecules with apparent m.w. of 49,000 and 43,000, respectively. Here we show that both proteins have a protein backbone m.w. of 33,000. In addition, T6 and M241 have a large portion of their peptides in common. When we compared the protein backbone m.w. of T6 and M241 with the murine beta-2m-associated thymus leukemia (TL) antigens, we found a considerable difference in size, suggesting that T6 and M241 may not be human homologues of TL antigens and constitute a novel type of major histocompatibility (MHC) class I antigens. 相似文献
24.
25.
A specific rabbit antibody against the 4-azido-2-nitrophenyl determinant was photo-labelled by the homologous hapten ε-(4-azido-2-nitrophenyl)-l-lysine, and by the close structural isomer ε-(5-azido-2-nitrophenyl)-l-lysine. The extents of covalent labelling of the antibody-binding site were assessed by using radioactive haptens and exhaustive displacement dialysis, which leaves the unlabelled sites empty but largely intact. A single photolysis of hapten–antibody complex suffices to label those sites that are capable of being labelled. Although there is considerable overlap among sub-populations of antibody that will bind the two haptens non-covalently, sites that can be covalently labelled by one reagent cannot be labelled by the other. 相似文献
26.
Spencer E. Knowles Ivan G. Jarrett Owen H. Filsell F. John Ballard 《The Biochemical journal》1974,142(2):401-411
1. In an attempt to define the importance of acetate as a metabolic precursor, the activities of acetyl-CoA synthetase (EC 6.2.1.1) and acetyl-CoA hydrolase (Ec 3.1.2.1) were assayed in tissues from rats and sheep. In addition, the concentrations of acetate in blood and liver were measured, as well as the rates of acetate production by tissue slices and mitochondrial fractions of these tissues. 2. Acetyl-CoA synthetase occurs at high activities in heart and kidney cortex of both species as well as in rat liver and the sheep masseter muscle. The enzyme is mostly in the cytosol fraction of liver, whereas it is associated with the mitochondrial fraction in heart tissue. Both mitochondrial and cytosol activities have a K(m) for acetate of 0.3mm. Acetyl-CoA synthetase activity in liver was not altered by changes in diet, age or alloxan-diabetes. 3. Acetyl-CoA hydrolase is widely distributed in rat and sheep tissues, the highest activity being found in liver. Essentially all of the activity in liver and heart is localized in the mitochondrial fraction. Hepatic acetyl-CoA hydrolase activity is increased by starvation in rats and sheep and during the suckling period in young rats. 4. The concentrations of acetate in blood are decreased by starvation and increased by alloxan-diabetes in both species. The uptake of acetate by the sheep hind limb is proportional to the arterial concentration of acetate, except in alloxan-treated animals, where uptake is impaired. 5. Acetate is produced by liver and heart slices and also by heart mitochondrial fractions that are incubated with either pyruvate or palmitoyl-(-)-carnitine. Liver mitochondrial fractions do not form acetate from either substrate but instead convert acetate into acetoacetate. 6. We propose that acetate in the blood of rats or starved sheep is derived from the hydrolysis of acetyl-CoA. Release of acetate from tissues would occur under conditions when the function of the tricarboxylic acid cycle is restricted, so that the circulating acetate serves to redistribute oxidizable substrate throughout the body. This function is analogous to that served by ketone bodies. 相似文献
27.
The inhibition of pepsin-catalysed reactions by products and product analogues. Kinetic evidence for ordered release of products 总被引:4,自引:4,他引:0 下载免费PDF全文
1. The inhibition of pepsin-catalysed hydrolysis of N-acetyl-l-phenylalanyl-l-phenylalanylglycine by products and product analogues was studied. 2. The non-competitive nature of the inhibition by the product N-acetyl-l-phenylalanine confirms an ordered release of products, and points to a common mechanism (involving an amino-enzyme) for pepsin-catalysed transpeptidation and hydrolysis reactions. 3. N-Acetyl-l-phenylalanine ethyl ester is also a non-competitive inhibitor, but here the inhibition is of the ;dead-end' type. No ethanol is detectable in reaction mixtures, indicating that this ester cannot act as an amino group acceptor in a transpeptidation process. 4. The same is true for N-methanesulphonyl-l-phenylalanine methyl and methyl thiol esters. No methanethiol is liberated when the methyl thiol ester is present as an inhibitor of the hydrolytic reaction, and the hope that such a thiol ester would effectively trap the amino-enzyme was not fulfilled. 相似文献
28.
Histological observations were made on normal and mutant strains of safflower in order to compare the development of the “fibrous” tissues among the various strains. The fibers of the vascular bundles of normal and F1's from crosses between normal and thin-hull mutant types had well-developed secondary cell walls, but they appeared reduced in mutant types. The anthers of all types showed a similar pattern of differentiation and maturation up to the final stages of tapetal breakdown when secondary walls and rib formations appeared in the connective regions and endothecial cells of the normal, striped and F1 types. These formations were absent from the thin-hull mutants. In both types dehiscence took place along a longitudinal fissure at the junction of the pollen sacs of one lobe of the anther. The anther flaps of normal types opened abruptly, thus effectively bringing the pollen into contact with the elongating style. Those of the mutants collapsed in place preventing the release of pollen. Hulls of the mutant strains were thin because cells were not sclerified during differentiation of the pericarp. Striped hulls resulted from the additional localization of secretory canals in the pericarp of the striped mutant. 相似文献
29.
1. The binding of three competitive inhibitors, N-acetyl-d-tryptophan, N-acetyl-l-tryptophan and N-acetyl-d-tryptophan amide, to alpha-chymotrypsin was studied over the pH range 2.20-9.65 by the technique of equilibrium dialysis. 2. Within the limits of the experimental method, the binding of the uncharged amide inhibitor is independent of pH over the range investigated. 3. The binding of each of the enantiomeric acids is dependent on the ionization of a group on the free enzyme, of apparent pK(a)7.3. 4. It is shown that the ionizing group results in the active site of the enzyme developing a net negative charge above pH7.3. 5. The enzyme groups responsible are tentatively identified, and the significance of the binding constants with respect to the enzymic catalysis is discussed. 相似文献
30.
1. The synthesis of three substrates of alpha-chymotrypsin of closely similar steric requirements but different charge type is reported. 2. The interaction of these compounds [SS-dimethyl-(l-3-carboxymethyl-3-acetamido)propyl sulphonium iodide, l-2-acetamido-5-methylhexanoic acid methyl ester and N-acetyl-l-glutamic acid alpha-methyl ester] with alpha-chymotrypsin has been studied. 3. For the charged substrates, values of k(0) are two orders of magnitude smaller than, and values of K(m) two orders of magnitude larger than, the corresponding values for the uncharged isostere. 4. The results are interpreted in terms of the known specificity of the enzyme, and the relationship between binding and kinetic specificities is discussed. 相似文献