全文获取类型
收费全文 | 126篇 |
免费 | 16篇 |
专业分类
142篇 |
出版年
2022年 | 3篇 |
2021年 | 2篇 |
2020年 | 1篇 |
2018年 | 3篇 |
2016年 | 5篇 |
2015年 | 6篇 |
2014年 | 4篇 |
2013年 | 13篇 |
2012年 | 9篇 |
2011年 | 9篇 |
2010年 | 10篇 |
2009年 | 5篇 |
2008年 | 6篇 |
2007年 | 4篇 |
2006年 | 8篇 |
2005年 | 3篇 |
2004年 | 4篇 |
2003年 | 2篇 |
2002年 | 6篇 |
2001年 | 5篇 |
2000年 | 2篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1995年 | 1篇 |
1994年 | 2篇 |
1992年 | 1篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1987年 | 2篇 |
1985年 | 3篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1982年 | 3篇 |
1981年 | 3篇 |
1979年 | 1篇 |
1978年 | 2篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1973年 | 2篇 |
排序方式: 共有142条查询结果,搜索用时 15 毫秒
131.
van der Knaap MS van Berkel CG Herms J van Coster R Baethmann M Naidu S Boltshauser E Willemsen MA Plecko B Hoffmann GF Proud CG Scheper GC Pronk JC 《American journal of human genetics》2003,73(5):1199-1207
Leukoencephalopathy with vanishing white matter, also called "childhood ataxia with central nervous system hypomyelination," is the first human disease related to mutations in any of the five genes encoding subunits of eukaryotic initiation factor eIF2B or any translation factor at all. eIF2B is essential in all cells of the body for protein synthesis and the regulation of this protein synthesis under different stress conditions. It is surprising that mutations in the eIF2B genes have been reported to lead to abnormalities of the white matter of the brain only, although it has been shown recently that ovarian failure may accompany the leukoencephalopathy. Another surprising observation is that the onset of the disease varies from early childhood to adulthood, with the exception of Cree leukoencephalopathy, a disease related to a particular mutation in one of the eIF2B genes, which invariably has its onset within the first year of life. We analyzed the eIF2B genes of nine patients with an antenatal- or early-infantile-onset encephalopathy and an early demise and found mutations in eight of the patients. In addition to signs of a serious encephalopathy, we found oligohydramnios, intrauterine growth retardation, cataracts, pancreatitis, hepatosplenomegaly, hypoplasia of the kidneys, and ovarian dysgenesis. Until now, no evidence had been found for a genotype-phenotype correlation, but the consistently severe phenotype in affected siblings among our patients and in Cree encephalopathy patients suggests an influence of the genotype on the phenotype. 相似文献
132.
133.
van der Knaap Willem O. van Leeuwen Jacqueline F. N. Fahse Lorenz Szidat Sönke Studer Tanja Baumann Joël Heurich Marco Tinner Willy 《Vegetation History and Archaeobotany》2020,29(2):277-295
Vegetation History and Archaeobotany - National parks are supposed to protect large-scale ecological processes, along with species and ecosystems. Detailed knowledge about past vegetation and... 相似文献
134.
A new class of tobacco chitinases homologous to bacterial exo-chitinases displays antifungal activity 总被引:14,自引:0,他引:14
Leo S. Melchers Marion Apotheker-de Groot Jan A. van der Knaap Anne S. Ponstein Marianne B. Sela-Buurlage John F. Bol Ben J.C. Cornelissen Peter J.M. van den Elzen Huub J.M. Linthorst 《The Plant journal : for cell and molecular biology》1994,5(4):469-480
A novel chitinase gene of tobacco was isolated and characterized by DNA sequence analysis of a genomic clone and a cDNA clone. Comparative sequence analysis of both clones showed an identity of 94%. The proteins encoded by these sequences do not correspond to any of the previously characterized plant chitinases of classes I–IV and are designated as class V chitinases. Comparison of the chitinase class V peptide sequence with sequences in the Swiss Protein databank revealed significant sequence similarity with bacterial exo-chitinases from Bacillus circulans, Serratia marcescens and Streptomyces plicatus. It was demonstrated that class V chitinase gene expression is induced after treatment of tobacco with different forms of stress, like TMV-infection, ethylene treatment, wounding or ultraviolet irradiation. Two related chitinase class V proteins of 41 and 43 kDa were purified from Samsun NN tobacco leaves inoculated with tobacco mosaic virus. The proteins were purified by Chelating Superose chromatography and gel filtration. In vitro assays demonstrated that class V chitinases have endo-chitinase activity and exhibit antifungal activity toward Trichoderma viride and Alternaria radicina. In addition, it was shown that class V chitinase acts synergistically with tobacco class I β-1,3-glucanase against Fusarium solani germlings. 相似文献
135.
Connor Simon E. van Leeuwen Jacqueline F. N. van der Knaap W. O. Akindola Rufus B. Adeleye Matthew A. Mariani Michela 《Vegetation History and Archaeobotany》2021,30(5):583-594
Vegetation History and Archaeobotany - Understanding the significance of pollen diversity is key to reconstructing plant diversity over long timescales. Here we present quantitative pollen-plant... 相似文献
136.
137.
The structure and function of the cell coat of the blood cells (amoebocytes) of the freshwater snail Lymnaea stagnalis were studied with ultrahistochemical tests, including concanavalin A (Con A) labeling, and with in vitro phagocytosis experiments. The cell coat is intensely stained by ruthenium red and tannic acid. The cells possess binding sites for Con A. Proteolytic enzymes destroy the receptors for Con A and totally inhibit the phagocytic activity of amoebocytes. Incubation experiments with proteases, carbohydrases, and inhibition sugars revealed that (1) the Con A binding sites are anchored in the plasma membrane by proteins, and (2) glucose, fructose, mannose, and to a lesser extent N-acetylglucosamine and N-acetylgalactosamine, inhibit the binding of Con A to amoebocytes, suggesting that these carbohydrates might form part of these binding sites. 相似文献
138.
At L. Hof Jelle Nauta Erik R. van der Knaap Michiel A. A. Schallig D. Peter Struwe 《Journal of electromyography and kinesiology》1992,2(4):203-216
The relation between changes in potential and kinetic energy in a seven-segment model of the human body and the work of m. triceps surae was investigated in four subjects walking on a treadmill at speeds between 0.5 and 2.0 m/s. Segment energy levels were determined by means of tachometers attached with strings to various points on the subject's body. Muscle work was assessed by electromyogram to force processing. M. triceps surae is active during stance, first doing negative (eccentric) work and ending with a short period of positive (concentric) work at “push-off”. It turned out that in normal walking these muscles provide the major part of positive work for the initiation of swing at push-off. Only at large step lengths, when push-off starts well before contralateral heel contact, is there a minor pushing forward of the trunk. In the negative work phase, m. triceps surae seem to check the forward speed of the trunk. A related decrease of trunk kinetic energy is not present, however, but this may be obscured by the simultaneous action of m. quadriceps femoris and, in a later stage, by a transfer of energy from the decelerating contralateral (swing) leg to the trunk. Energy of the trunk segment shows a sharp decline in double stance and a more gradual increase in the first half of single stance. Evidence is given that this effect is due to quadriceps action in the knee flexion-extension movement during stance. The presented results are incorporated in a general picture of energy flows in human walking. 相似文献
139.
140.