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22.
Pierre Bize Aurélie Klopfenstein Caroline Jeanneret Alexandre Roulin 《Journal of Ornithology》2007,148(4):387-393
The Alpine swift (Apus melba) forages on insects caught exclusively on the wing, implying that dependent nestlings face acute food shortage in periods
of cold and rainy weather. Therefore, there should be strong selection on nestling swifts to evolve physiological strategies
to cope with periods of undernutrition. We have investigated intra-individual changes in nestling pectoral muscle and body
temperature in response to a 1-week period of inclement weather. The pectoral muscle is the largest reserves of proteins,
and nestlings have to devote a large amount of energy in the maintenance of body temperature. The results show that nestling
pectoral muscle size and body temperature were significantly reduced during the episode of inclement weather. Assuming that
these physiological changes are adaptive, our study suggests that nestling swifts spare energy by a pronounced reduction (up
to 18°C) in body temperature and use proteins from the pectoral muscle as a source of extra energy to survive prolonged periods
of fasting. 相似文献
23.
Terry Klopfenstein 《Animal Feed Science and Technology》1996,60(3-4):201-207
The use of distillers byproducts as energy sources would expand the market for these byproducts. Drying of these byproducts may produce changes that affect the nutritional value. A series of experiments was conducted to evaluate distillers byproducts as an energy source for finishing cattle and to compare wet vs. dried byproducts. The energy value of wet distillers byproducts was 132 to 174% the value of dry rolled corn for finishing cattle when the byproducts were fed as 40% of the diet dry matter. Distillers dried grains plus solubles were 130% the energy value of corn. Acid detergent insoluble nitrogen level of the dried grains did not affect feeding value. Distillers dried grains plus solubles were equal in protein value to wet byproducts when fed to growing calves. Acid detergent insoluble nitrogen level did not affect protein utilization of dried distillers byproducts. 相似文献
24.
Terry Klopfenstein 《Animal Feed Science and Technology》1996,60(3-4):191-199
Grazing cattle require rumen degradable protein to meet the needs of the microbial population and rumen undegradable protein to meet the productive needs of the animal. The need for rumen degradable protein is about 130 g kg−1 of digestible organic matter. However, when low-quality forage is consumed, rate of passage is reduced, microbial growth rate is reduced and conversion efficiency of microbial growth is reduced. This probably lowers the rumen degradable protein requirement. Escape protein is necessary to meet cattle requirements for growth or lactation. Microbial protein is usually adequate for animals at or near maintenance. The protein in both cool-season and warm-season grasses is highly degraded. Therefore, growing cattle and lactating cows responded to supplemental escape protein. Knowledge of the rumen degradable and undegradable protein contents of grazed forages is essential to more precisely predict animal performance and the animal response to protein supplements. 相似文献
25.
JB Parentes-Vieira PV Lopes-Costa CG Pires AR dos Santos JD Pereira-Filho BB da Silva 《International Seminars in Surgical Oncology : ISSO》2007,4(1):22
Background
The objective of this study was to evaluate angiogenesis according to CD34 antigen expression in estrogen receptor (ER)-positive and negative breast carcinomas.Methods
This study comprised 64 cases of infiltrating ductal carcinoma in postmenopausal women divided into two groups: Group A: ER-positive, n = 35; and Group B: ER-negative, n = 29. The anti-CD34 monoclonal antibody was used as a marker for endothelial cells. Microvessel count was carried out in 10 fields per slide using a 40× objective lens (magnification 400×). Statistical analysis of the data was performed using Student's t-test (p < 0.05).Results
The mean number of vessels stained with the anti-CD34 antibody in the estrogen receptor-positive and negative tumors was 23.51 ± 1.15 and 40.24 ± 0.42, respectively. The number of microvessels was significantly greater in the estrogen receptor-negative tumors (p < 0.001).Conclusion
ER-negative tumors have significantly greater CD34 antigen expression compared to ER-positive tumors.26.
Teng YK Verburg RJ Verpoort KN Diepenhorst GM Bajema IM van Tol MJ Jol-van der Zijde EC Toes RE Huizinga TW van Laar JM 《Arthritis research & therapy》2007,9(5):R106
In order to identify pathogenic correlates of refractory rheumatoid arthritis (RA), antibodies against anti-cyclic citrullinated
protein (ACPAs) were investigated in RA patients in whom the dysregulated immune system had been ablated by high-dose chemotherapy
(HDC) and autologous haematopoietic stem cell transplantation (HSCT). Six patients with refractory RA were extensively characterized
in terms of levels of total immunoglobulins, RA-specific autoantibodies (ACPAs and rheumatoid factor) and antibodies against
rubella, tetanus toxoid (TT) and phosphorylcholine before and after HDC plus HSCT. Additionally, the avidity of ACPAs was
measured before and after treatment and compared with the avidity of TT antibodies following repeated immunizations. Synovial
biopsies were obtained by arthroscopy before HDC plus HSCT, and analyzed by immunohistochemistry. In the three patients with
clinically long-lasting responses to HDC plus HSCT (median 423 days), significant reductions in ACPA-IgG levels after therapy
were observed (median level dropped from 215 to 34 arbitrary units/ml; P = 0.05). In contrast, stable ACPA-IgG levels were observed in three patients who relapsed shortly after HDC plus HSCT (median
of 67 days). Clinical responders had ACPA-IgG of lower avidity (r = 0.75; P = 0.08) and higher degree of inflammation histologically (r = 0.73; P = 0.09). Relapse (after 38 to 530 days) in all patients was preceded by rising levels of low avidity ACPA-IgG (after 30 to
388 days), in contrast to the stable titres of high avidity TT antibodies. In conclusion, humoral autoimmune responses were
differentially modulated by immunoablative therapy in patients with synovial inflammation and low avidity ACPA-IgG autoantibodies
as compared with patients with high levels of high avidity ACPA-IgG. The distinct clinical disease course after immunoablative
therapy based on levels and avidity of ACPA-IgG indicates that refractory RA is not a single disease entity. 相似文献
27.
Bize P Gasparini J Klopfenstein A Altwegg R Roulin A 《Evolution; international journal of organic evolution》2006,60(11):2370-2380
Two mutually exclusive hypotheses have been put forward to explain the evolution and adaptive function of melanin-based color traits. According to sexual selection theory melanism is a directionally selected signal of individual quality, whereas theory on the maintenance of genetic polymorphism proposes that alternative melanin-based variants achieve equal fitness. Alpine swift (Apus melba) males and females have a conspicuous patch of white feathers on the breast with their rachis varying continuously from white to black, and hence the breast varies from white to striated. If this trait is a sexually selected signal of quality, its expression should be condition dependent and the degree of melanism directionally selected. If variation in melanism is a polymorphism, its expression should be genetically determined and fitness of melanin-based variants equal. We experimentally tested these predictions by exchanging eggs or hatchlings between randomly chosen nests and by estimating survival and reproduction in relation to melanism. We found that breast melanism is heritable and that the environment and body condition do not significantly influence its expression. Between 5 and 50 days of age nestlings were heavier and their wings longer when breast feathers of their biological father were blacker, and they also fledged at a younger age. This shows that aspects of offspring quality covary positively with the degree of melanism. However, this did not result in directional selection because nestling survival and recruitment in the local breeding population were not associated with father breast melanism. Furthermore, adult survival, age at first reproduction and probability of skipping reproduction did not covary with the degree of melanism. Genetic variation in breast melanism is therefore maintained either because nonmelanic males achieve fitness similar to melanic males via a different route than producing fast-growing offspring, or because the advantage of producing fast-growing offspring is not sufficiently pronounced to result in directional selection. 相似文献
28.
29.
High-throughput approaches are beginning to have an impact on many areas of yeast biology. Two recent studies, using different experimental platforms, provide insight into new pathways involved in the response of yeast to DNA damage. 相似文献
30.
Uniparentally inherited mitochondrial (mt)DNA and chloroplast (cp)DNA microsatellites (cpSSRs) were used to examine population genetic structure and biogeographic patterns of bird-dispersed seed and wind-disseminated pollen of whitebark pine (Pinus albicaulis Engelm.). Sampling was conducted from 41 populations throughout the range of the species. Analyses provide evidence for an ancestral haplotype and two derived mtDNA haplotypes with distinct regional distributions. An abrupt contact zone between mtDNA haplotypes in the Cascade Range suggests postglacial biogeographic movements. Among three cpSSR loci, 42 haplotypes were detected within 28 cpSSR sample populations that were aggregated into six regions. Analysis of molecular variance (amova) was used to determine the hierarchical genetic structure of cpSSRs. amova and population pairwise comparisons (FST ) of cpSSR, and geographical distribution of mtDNA haplotypes provide insights into historical changes in biogeography. The genetic data suggest that whitebark pine has been intimately tied to climatic change and associated glaciation, which has led to range movements facilitated by seed dispersal by Clark's nutcracker (Nucifraga columbiana Wilson). The two hypotheses proposed to explain the genetic structure are: (i) a northward expansion into Canada and the northern Cascades in the early Holocene; and (ii) historical gene flow between Idaho and the Oregon Cascades when more continuous habitat existed in Central Oregon during the late Pleistocene. Genetic structure and insights gained from historical seed movements provide a basis on which to develop recovery plans for a species that is at risk from multiple threats. 相似文献