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411.
ESTELA NOVAK EDNA FREYMULLER HAAPALAINEN SOLANGE DA SILVA JOSE FRANCO DA SILVEIRA 《The Journal of eukaryotic microbiology》1988,35(3):375-378
Here we describe a method that allows the isolation of intact trypanosomatid symbionts in amounts sufficient for biochemical analysis. The isolated symbionts retain their characteristic morphological features and are reasonably free of subcellular debris. They actively incorporate [3H]leucine and [35S]methionine into proteins. Chloramphenicol and rifampicin at 50 μg/ml almost completely inhibit the incorporation of protein precursors. The inhibition of protein synthesis by the antibiotics provides direct evidence for the existence of a prokaryotic protein-synthesizing system in this unusual intracellular structure. The pattern of protein synthesis of the isolated symbionts is complex. Several symbiont polypeptides are absent or poorly represented in the flagellate. 相似文献
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The inter-relationship between the age of carrot roots at harvest and infection by Mycocentrospora acerina in storage 总被引:1,自引:0,他引:1
Infection of carrot roots by Mycocenlrospora acerina in chill storage (3.5 °C) following inoculation with chlamydospores was studied in 1973–74 and 1974–75. AREAS of intact periderm were only rarely infected, and the high level of periderm resistance predominated over other variables. However, wound infection tended to increase with depth of wound and with increasing age of the plants at harvest. Irrespective of age of root or depth of wound, roots were comparatively resistant to infection at harvest and early in storage, resistance being expressed as a restriction of mycelial growth on the wound surface or localisation of the lesion. Increasing susceptibility with time in storage, depth of wounds, or age at harvest, resulted in larger numbers of inoculated sites becoming infected and a more rapid development from localised to progressive lesions. 相似文献
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C. Dudok De Wit E. Borst-Eilers Ch.M. v. d. Weerdt G. J. Kloosterman 《BMJ (Clinical research ed.)》1968,4(5629):477-479
A controlled clinical trial was carried out to test the effectiveness of a comparatively low dose of anti-D immunoglobulin (250 μg) in preventing rhesus immunization.In the control group 17 out of 329 women (5%) formed rhesus antibodies, whereas in the treated group only 3 out of 333 women (0.9%) showed active immunization, all three of whom had an exceptionally large transplacental bleeding. 相似文献
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