Changes in the lipid component of dried doughnut mixtures during storage

Changes in various indices of lipid components of dried doughnut mixtures during their joint or separate 3-month storage were studied. Methyl oleate oxidation model was used to determine the prooxidant properties of the initial mixture. The antioxidant activity, inhibition of oxidation, and inhibitory properties of lipids were shown to be enhanced during storage. The composition of phospholipids varied considerably due to changes in the degree of unsaturation of lipids in the mixture during storage. Joint storage of components resulted in greater stability and better preservation of lipid components (as compared to their separate storage).     

Shift of grey seal subspecies boundaries in response to climate,culling and conservation

Identifying the processes that drive changes in the abundance and distribution of natural populations is a central theme in ecology and evolution. Many species of marine mammals have experienced dramatic changes in abundance and distribution due to climatic fluctuations and anthropogenic impacts. However, thanks to conservation efforts, some of these species have shown remarkable population recovery and are now recolonizing their former ranges. Here, we use zooarchaeological, demographic and genetic data to examine processes of colonization, local extinction and recolonization of the two northern European grey seal subspecies inhabiting the Baltic Sea and North Sea. The zooarchaeological and genetic data suggest that the two subspecies diverged shortly after the formation of the Baltic Sea approximately 4200 years bp , probably through a gradual shift to different breeding habitats and phenologies. By comparing genetic data from 19th century pre‐extinction material with that from seals currently recolonizing their past range, we observed a marked spatiotemporal shift in subspecies boundaries, with increasing encroachment of North Sea seals on areas previously occupied by the Baltic Sea subspecies. Further, both demographic and genetic data indicate that the two subspecies have begun to overlap geographically and are hybridizing in a narrow contact zone. Our findings provide new insights into the processes of colonization, extinction and recolonization and have important implications for the management of grey seals across northern Europe.     

Use of factor-H sera for the differentiation of the flagellar antigens of Escherichia coli serological variants

The use of factor sera permitting the differentiation of the variants, described in earlier. works, among the flagellar antigens of E. coli, formally denoted as H2, H4, H7, H10 and H34 in accordance with their official nomenclature, has made it possible to reveal that each of these variants is widely spread among E. coli and occurs in bacteria of different O-groups. Besides, this study has shown the possibility of subdividing a number of formal H: O types into 2 or more serovars on the basis of differences in the factor composition of their antigens. The results obtained in this study suggest that in the process of the evolution of E. coli H-antigen variants differing in their factor composition have been formed as independent varieties; therefore, these variants do not reflect the features characteristic of individual strains, but constitute one of the diagnostic signs of serological classification, i. e. the differentiation of the species into various serovars.     

NMR and water relaxation of floral honey samples from wild and domesticated bees

The aqueous solutions of Bashkir floral honey of wild and domesticated bees were studied with high-resolution ¹H and ¹³C NMR and nuclear magnetic relaxation. NMR was shown to provide only qualitative data on the composition of the studied honey samples. Data on the composition of the minor components (amino acids), as well as the mobility of water protons in honey, indicate that the distinctions between honey from wild and domesticated bees are due to both the honey composition and the difference in the interactions of components with one another and with water.     

The effect of preparations of non-histone chromosomal proteins on the expression of hetero-organic membrane antigens of kidney origin in a primary rat hepatocyte culture

The expression of membrane hetero-organic antigens of kidney origin, associated with the rat hepatomas in primary culture of intact adult rat hepatocytes, was investigated by means of the indirect immunofluorescence method using a specific immune serum. These antigens were observed on the membrane of some hepatocytes after their contact with nonhistone chromosomal proteins (NHCP), which were obtained from the kidney of intact rats from cells of hepatoma 27 and Zajdela hepatoma, or from the carcinogenic liver after a single diethylnitrosamine injection. Negative results were obtained after the incubation of hepatocytes in the medium lacking some of NHCP, or in that with NHCP obtained from the liver of intact rats.     

Cytogenetic effect of barbiturate anesthetizing substances in a human peripheral blood leukocyte culture

It is found that hexenal and sodium thiopental in vitro produced a two-fold increase of frequency of chromosome aberrations as compared with the control and this effect was not dose-dependent. The anesthetics under study affected in vitro 3H-thymidine incorporation into DNA of lymphocytes, and a ten-fold hexenal dose intensified 3H-thymidine incorporation. The frequency of chromosome aberrations in vivo was at the level of the spontaneous mutation after use of sodium thiopental and slightly increased hexenal.     

Mechanism of the radioprotective effect of catecholamine receptor agonists. Inclusion in the radioprotective effect of different subtypes of beta-adrenoreceptors

Isoproterenol of ED50 = 0.16 mumol/kg is highly effective in protecting mice against ionizing radiation and has the high therapeutical index. There are three new indications that isoproterenol exerts its radioprotective action via beta-adrenoceptors. They are: the effect of isoproterenol is prevented by three additional beta-adrenoceptor antagonists, the isoproterenol effect is reproduced by the seven studied beta-adrenoceptor agonists of different chemical structure, and with chemical analogs which fail to stimulate beta-adrenoceptors the radioprotective effect is absent. Both beta 1 and beta 2 adrenoceptor agonists are protective agents. Mechanisms of the radioprotective action are discussed.