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151.
WASP is a remodeler of the actin cytoskeleton, but its mechanistic contribution to neutrophil migration is unclear. In this issue, Brunetti et al. (2021. J. Cell Biol. https://doi.org/10.1083/jcb.202104046) show that WASP is recruited to substrate-induced membrane deformations near the cell front, where it induces Arp2/3 complex–mediated local actin assembly to direct migration.Neutrophils are the most abundant type of white blood cells in humans and constitute an important first line of defense of the innate immune system. Neutrophils exit the blood stream in response to chemoattractants that signal danger in the form of damage, infection, or inflammation, ultimately removing dangerous particles by phagocytosis. Efficient migration is crucial to properly execute such functions, but how precisely the coordination of polarity establishment and complex shape changes needed for migratory processes occurs in these cells is just beginning to be elucidated. The dynamic remodeling of actin filaments, which can differentially assemble and disassemble at both ends, is key to the development of pulling and pushing forces below the plasma membrane of most eukaryotic cells or on the surfaces of their intracellular organelles (1).Actin filaments are organized into either bundles or networks, with networks most commonly generated and maintained by the continuous filament branching activity of the actin-related protein 2/3 (Arp2/3) complex (2). Arp2/3 complex–driven branching in actin networks has often been associated with the development of pushing forces, even in a mechano-responsive fashion (3, 4), and less so linked with pulling or traction forces as developed, for instance, by adhesions. The heteropentameric Arp2/3 complex, which catalyzes the formation of daughter filaments off the sides of mother filaments, is intrinsically inactive, but can be activated by nucleation promoting factors (NPFs). The canonical NPFs include the Wiskott-Aldrich syndrome protein (WASP), its ubiquitous orthologue neural WASP (N-WASP), three WASP family verprolin-homologous protein (WAVE) isoforms as well as WASH, WHAMM, and JMY (5). The C-termini of all these factors physically interact with the Arp2/3 complex, driving it into an active conformation, and aid daughter filament polymerization by adding actin monomers onto Arp-2 and -3, forming the base of the branch. Coordinating the branching of daughter filaments by Arp2/3 complex and their elongation appears to be a major function of NPFs such as WASP. The role of WASP in stimulating actin assembly and the observation that patients with Wiskott-Aldrich syndrome harboring mutations in this gene have white blood cells that are unable to reach the sites of infections (6) points to WASP as a regulator of guided migration of immune cells. In this issue, Brunetti et al. establish the precise cellular function by which WASP controls neutrophil migration: WASP is recruited to sites of inward membrane deformations to stimulate the formation of spot-like actin structures that aid in the adhesion to or in the grabbing onto extracellular structures of high diversity and flexibility such as extracellular matrix fibrils, thus linking substrate topology, cell polarity, and migration (7).The authors first determined the spatiotemporal dynamics of EGFP-tagged, endogenous WASP in the human neutrophil–like cell line HL-60 using total internal reflection fluorescence microscopy. WASP formed puncta that were largely devoid of clathrin accumulation, thus likely not embodying endocytic pits, and exogenously triggered by submicron-sized beads, microfabricated ridges, or collagen fibers. Substratum-associated WASP puncta exhibited adhesive functions, as integrin inactivation by ion chelation largely dissociated them, stimulating their sliding and centripetal displacement. Surprisingly, the prominent WASP accumulation to beads below the plasma membrane was biased to the front half of the cell, and coincided with a gradient of activity of its major activator, the Rho GTPase Cdc42 from front to rear. This observation led Brunetti et al. to postulate WASP as a factor linking substrate topology to cell polarization and effective migration.A closer look at the recruitment of WASP to inward plasma membrane invaginations (positive membrane curvature) stimulated by submicron-sized beads revealed two patterns of association: either following the bead bodies as U-shaped accumulations or to the necks of invaginations reminiscent of endocytic pits, which could reflect later stages of bead–plasma membrane interactions. Although the mechanistic significance of these distinct association patterns remains elusive, it was clear that WASP favored associations at the plasma membrane with sites of comparably high, positive curvature (small, 100-nm-diameter beads more attractive than larger beads, for instance) and in a punctate fashion (even along ridges). In our view, this much diverges from WAVE isoforms exhibiting a prominent, more linear association with the rapidly protruding membranes of negative curvature in lamellipodia (8). Finally, the authors showed that Cdc42 loss-of-function diminished WASP puncta at the cell front, and that lack of WASP reduced actin puncta formation and the frequency of Arp2/3 complex accumulation at beads. WASP-null cells migrated less in the direction of ridges, although their perpendicular movement across the nanopatterns was not affected—indeed, it was increased. These observations connect the role of WASP in reading substrate topology to Arp2/3-dependent actin filament branching and network formation. The findings of Brunetti et al. appear to be of broader impact and relevance than just being a human neutrophil-specific phenomenon—a separate study has recently shown that sliding, WASP-dependent, punctate adhesion sites in murine dendritic cells and T cells, which are triggered in response to mechanical load, polymerize actin networks orthogonally to the plasma membrane, and aid in squeezing and dragging these cells through dense tissues (9).Taking all this together, the study by Brunetti et al. highlights that at least immune cells primarily respond to mechanical impact and indentations of their plasma membranes with rapid accumulation of WASP and Arp2/3 complex–dependent actin assembly, thereby pushing back or grabbing extracellular material to push and pull themselves forward during migration through the complex, dense, and three-dimensional environments on their way through tissues (Fig. 1).Open in a separate windowFigure 1.Neutrophils use WASP puncta to crawl like mountain climbers. Punctate accumulation of WASP and consequently F-actin brings about friction points that support pushing and pulling during cell translocation. In the front half of the cell, formation of these points strictly depends on Cdc42 mediating WASP activation and focal actin assembly. Even on substrates that would allow continuous adhesion (ridges or collagen fibers), WASP is recruited in a punctate fashion and preferentially to the front half of the cell that is dominated by Cdc42 activity signaling to cell polarity. Upon contact, movable objects like beads lead to a strong WASP/actin response potentially culminating in object envelopment.Interestingly, according to Brunetti et al., mechanisms of WASP/N-WASP recruitment to inward membrane deformations might even be conserved during early stages of formation of podosomes and invadopodia, which are WASP- and N-WASP–dependent, both protrusive and adhesive structures operating in matrix degradation of hematopoietic and cancer cells, respectively (10). This hypothesis, however, remains to be experimentally validated. Furthermore, additional questions remain unsolved, including the precise signals, aside from Cdc42, contributing to WASP accumulation and turnover at the plasma membrane. Where does the bias for focal, punctate WASP accumulation come from? WASP family NPFs are unlikely to themselves harbor curvature-sensing activities, so which are the decisive factors for interaction with positively curved membranes? How is specificity brought about? The F-BAR domain-containing TOCA family proteins would have been potential candidates, but surprisingly, disruption of two of their most prominent members, FBP17 and CIP4, did not cause severe defects, as Brunetti et al. showed in this study. Future work will surely improve our insights into the differential sorting and subcellular functions of distinct NPFs in both immune and other cells. 相似文献
152.
Serena Donadi Joëlle Westra Ellen J. Weerman Tjisse van der Heide Els M. van der Zee Johan van de Koppel Han Olff Theunis Piersma Henk W. van der Veer Britas Klemens Eriksson 《Ecosystems》2013,16(7):1325-1335
The importance of positive effects of ecosystem engineers on associated communities is predicted to increase with environmental stress. However, incorporating such non-trophic interactions into ecological theory is not trivial because facilitation of associated species is conditional on both the type of engineer and the type of abiotic stress. We tested the influence of two allogenic ecosystem engineers (lugworms, Arenicola marina L. and cockles, Cerastoderma edule L.) on the main primary producers (microphytobenthos) of the tidal flats, under different abiotic stresses controlled by reefs of blue mussels (Mytilus edulis L.). We added 25,000 cockles or 2,000 lugworms to 5 × 5 m plots, both in a muddy site with high sedimentation rates located coastward of a mussel bed, and in a sandy site without mussels and characterized by high hydrodynamic stress. After a year, cockles increased algal biomass in the sandy area, but not in the mussel bed site, where high values were measured in all plots. However, lugworms did not affect algal biomass in any of the sites. Field measurements suggest that cockles outweighed negative effects of water currents in the site without mussels by locally increasing sediment stability, whereas mussels overruled the effects of cockles in the wake of the reefs through hydrodynamic stress alleviation and/or biodeposition. Our results suggest that non-trophic interactions by ecosystem engineering bivalves control primary production of intertidal areas, and that the sediment-stabilizing effect of cockles plays a crucial role where the overruling effects of mussel beds are not present. 相似文献
153.
154.
Klemens Klein 《Cell and tissue research》1931,14(3):481-516
Ohne Zusammenfassung
Mit 42 Textabbildungen 相似文献
155.
156.
Marama bean (Tylosema esculentum, Fabaceae), a potential arid-land legume crop from the Kalahari Desert of Botswana, was analyzed for protein, amino acids, oil, fatty acids, fiber, caloric value, trypsin inhibitor, and mineral content. Results indicate that the bean is adequate in these nutrients for a human diet, but the trypsin inhibitor activity should be destroyed prior to consumption. 相似文献
157.
Müller-Taubenberger A Vos MJ Böttger A Lasi M Lai FP Fischer M Rottner K 《European journal of cell biology》2006,85(9-10):1119-1129
Fluorescent proteins have proven to be excellent tools for live-cell imaging studies. In addition to green fluorescent protein (GFP) and its variants, recent progress was achieved in the development of monomeric red fluorescent proteins (mRFPs) that show improved properties in respect to maturation and intracellular fluorescence. mRFPmars, a red fluorescent protein designed especially for the use in Dictyostelium, has been employed to tag different proteins for live-cell investigations in Dictyostelium. mRFPruby, which differs in sequence from mRFPmars in four amino acids, has a codon usage optimised for the application in mammalian cells. Here, we show that both mRFP variants can also be applied for localisation studies in other organisms. mRFPmars was expressed in Hydra and fused to the Bcl-2 family protein Bax. mRFPruby in combination with histone 2B was expressed in Drosophila S2 cells to monitor mitosis. Using mouse cell lines, mRFPruby fused to beta-actin was assayed with high spatial resolution to study details of actin cytoskeleton dynamics. In addition, we demonstrate that both mRFP variants are also suitable for dual-colour microscopy in the different species. 相似文献
158.
159.
Klemens Pütz Andrea Raya Rey Adrián Schiavini Andrea P. Clausen Bernhard H. Lüthi 《Polar Biology》2006,29(9):735-744
Rockhopper penguins (Eudyptes chrysocome) breeding on Staten Island, Argentina, were satellite tracked in 2002 and 2003 during the onset of their winter migration. After their moult, the dispersal of 24 birds was monitored for a mean period of 50.0±40.3 days. Birds travelled at a mean velocity of 3.1±1.1 km/h. The mean minimum distance travelled was 1,640±1,425 km; the maximum distance to the colony was generally less than 1,000 km, although one bird travelled more than 2,000 km from the colony. The penguins dispersed over an area totalling about 1.3 million km2, ranging from 50 to 62°S and from 49°W in the Atlantic to 92°W in the Pacific, and covering polar, sub-polar and temperate waters in oceanic regions as well as shelf waters. Despite the very wide dispersal, both temporally and spatially, two important wintering grounds for rockhopper penguins from Staten Island could be identified, both located over shelf regions: one extended from Staten Island to the north along the coast of Tierra del Fuego up to the Magellan Strait; the other was located over the Burdwood Bank, an isolated extension of the Patagonian Shelf to the south of the Falkland Islands. The Drake Passage also appeared to be an important area for wintering penguins, although dispersal was far more widely spread. Comparison with data obtained during winter from rockhopper penguins originating from the Falkland Islands showed that the area off the coast of Tierra del Fuego was used more or less exclusively by birds from Staten Island, whereas the Burdwood Bank was shared with penguins coming from southern colonies in the Falkland Islands. The implications of these findings are discussed with regard to (a) opposing population trends of rockhopper penguins in the Southwest Atlantic, and (b) the urgent need to establish adequate conservation measures for species and habitat protection. 相似文献
160.
Sexual differentiation in Drosophila is regulated through alternative splicing of doublesex. Female-specific splicing is activated through the activity of splicing enhancer complexes assembled on multiple repeat elements. Each of these repeats serves as a binding platform for the cooperative assembly of a heterotrimeric complex consisting of the SR proteins Tra, Tra2 and 9G8. Using quantitative kinetic analyses, we demonstrate that each component of the enhancer complex is capable of recruiting the spliceosome. Surprisingly, Tra, Tra2 and 9G8 are much stronger splicing activators than other SR protein family members and their activation potential is significantly higher than expected from their serine/arginine content. 9G8 activates splicing not only through its RS domains but also through its RNA-binding domain. The RS domains of Tra and Tra2 are required but not sufficient for efficient complex assembly. Thus, the regulated assembly of the dsx enhancer complexes leads to the generation of an extended activation domain to guarantee the ‘all or none’ splicing switch that is required during Drosophila sexual differentiation. 相似文献