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Dual effect of acid pH on purinergic P2X3 receptors depends on the histidine 206 residue 总被引:1,自引:0,他引:1
Gerevich Z Zadori ZS Köles L Kopp L Milius D Wirkner K Gyires K Illes P 《The Journal of biological chemistry》2007,282(47):33949-33957
Whole cell patch clamp investigations were carried out to clarify the pH sensitivity of native and recombinant P2X(3) receptors. In HEK293 cells permanently transfected with human (h) P2X(3) receptors (HEK293-hP2X(3) cells), an acidic pH shifted the concentration-response curve for alpha,beta-methylene ATP (alpha,beta-meATP) to the right and increased its maximum. An alkalic pH did not alter the effect of alpha,beta-meATP. Further, a low pH value increased the activation time constant (tau(on)) of the alpha,beta-meATP current; the fast and slow time constants of desensitization (tau(des1), tau(des2)) were at the same time also increased. Finally, acidification accelerated the recovery of P2X(3) receptors from the desensitized state. Replacement of histidine 206, but not histidine 45, by alanine abolished the pH-induced effects on hP2X(3) receptors transiently expressed in HEK293 cells. Changes in the intracellular pH had no effect on the amplitude or time course of the alpha,beta-meATP currents. The voltage sensitivity and reversal potential of the currents activated by alpha,beta-meATP were unaffected by extracellular acidification. Similar effects were observed in a subpopulation of rat dorsal root ganglion neurons expressing homomeric P2X(3) receptor channels. It is suggested that acidification may have a dual effect on P2X(3) channels, by decreasing the current amplitude at low agonist concentrations (because of a decrease in the rate of activation) and increasing it at high concentrations (because of a decrease in the rate of desensitization). Thereby, a differential regulation of pain sensation during e.g. inflammation may occur at the C fiber terminals of small DRG neurons in peripheral tissues. 相似文献
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Gunnarsen Klara Cecilia Schjoerring Jan Kofod Gómez-Muñoz Beatriz de Neergaard Andreas Jensen Lars Stoumann 《Plant and Soil》2022,477(1-2):241-258
Plant and Soil - Plant-available silicon (Si) is limited in strongly weathered tropical soils. The aim of the study was to evaluate Si fertilisation as a strategy to improve phosphorus (P)... 相似文献
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The extracellular architecture of adherens junctions revealed by crystal structures of type I cadherins 总被引:1,自引:0,他引:1
Harrison OJ Jin X Hong S Bahna F Ahlsen G Brasch J Wu Y Vendome J Felsovalyi K Hampton CM Troyanovsky RB Ben-Shaul A Frank J Troyanovsky SM Shapiro L Honig B 《Structure (London, England : 1993)》2011,19(2):244-256
Adherens junctions, which play a central role in intercellular adhesion, comprise clusters of type I classical cadherins that bind via extracellular domains extended from opposing cell surfaces. We show that a molecular layer seen in crystal structures of E- and N-cadherin ectodomains reported here and in a previous C-cadherin structure corresponds to the extracellular architecture of adherens junctions. In all three ectodomain crystals, cadherins dimerize through a trans adhesive interface and are connected by a second, cis, interface. Assemblies formed by E-cadherin ectodomains coated on liposomes also appear to adopt this structure. Fluorescent imaging of junctions formed from wild-type and mutant E-cadherins in cultured cells confirm conclusions derived from structural evidence. Mutations that interfere with the trans interface ablate adhesion, whereas cis interface mutations disrupt stable junction formation. Our observations are consistent with a model for junction assembly involving strong trans and weak cis interactions localized in the ectodomain. 相似文献
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