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81.
Albert Leopold Müller Júlia Rosa de Rezende Casey R J Hubert Kasper Urup Kjeldsen Ilias Lagkouvardos David Berry Bo Barker J?rgensen Alexander Loy 《The ISME journal》2014,8(6):1153-1165
Microbial biogeography is influenced by the combined effects of passive dispersal and environmental selection, but the contribution of either factor can be difficult to discern. As thermophilic bacteria cannot grow in the cold seabed, their inactive spores are not subject to environmental selection. We therefore conducted a global experimental survey using thermophilic endospores that are passively deposited by sedimentation to the cold seafloor as tracers to study the effect of dispersal by ocean currents on the biogeography of marine microorganisms. Our analysis of 81 different marine sediments from around the world identified 146 species-level 16S rRNA phylotypes of endospore-forming, thermophilic Firmicutes. Phylotypes showed various patterns of spatial distribution in the world oceans and were dispersal-limited to different degrees. Co-occurrence of several phylotypes in locations separated by great distances (west of Svalbard, the Baltic Sea and the Gulf of California) demonstrated a widespread but not ubiquitous distribution. In contrast, Arctic regions with water masses that are relatively isolated from global ocean circulation (Baffin Bay and east of Svalbard) were characterized by low phylotype richness and different compositions of phylotypes. The observed distribution pattern of thermophilic endospores in marine sediments suggests that the impact of passive dispersal on marine microbial biogeography is controlled by the connectivity of local water masses to ocean circulation. 相似文献
82.
In vivo experiments in mice demonstrated that 5% CO2 content in the air inhaled did not change the labelling in autoradiograms from animals injected with [3H]uridine, [3H]orotic acid, [3H]hypoxanthine, [3H]lysine or [3H]cytidine. At 20% CO2 content there was a significant decrease in labelling of brain cells with [3H]uridine and [3H]cytidine, but not following [3H]lysine; there was no labelling of nerve cells with [3H]orotic acid or [3H]hypoxanthine, but a control group was not included. The labelling of choroid plexus and hepatocytes was independent of the CO2 concentration. A comparison of in vivo and in vitro experiments at 20% CO2 content showed a similar significant decrease in labelling of brain cells with [3H]uridine and [3H]cytidine. It is concluded that a metabolic change is the most appropriate explanation of the CO2 effect. 相似文献
83.
Kasper Urup Kjeldsen Matthias Obst Hiroaki Nakano Peter Funch Andreas Schramm 《Applied and environmental microbiology》2010,76(8):2657-2662
Two types of endosymbiotic bacteria were identified in the gastrodermis of the marine invertebrate Xenoturbella bocki (Xenoturbellida, Bilateria). While previously described Chlamydia-like endosymbionts were rare, Gammaproteobacteria distantly related to other endosymbionts and pathogens were abundant. The endosymbionts should be considered when interpreting the poorly understood ecology and evolution of Xenoturbella.Xenoturbella bocki is a benthic marine worm first described in 1949 and only found at a few geographical locations at apparently low population densities. It has a very simple body plan (Fig. (Fig.1A)1A) with a blind gut (cul-de-sac) and lacks coelomic cavities, a brain, and reproductive and excretory organs (35). Xenoturbella was originally classified as a flatworm, but molecular analyses have placed it within its own animal phylum, with various affiliations (reviewed in reference 34). The most recent phylogenetic analyses using mitochondrial genome and phylogenomic data indicate a position either within Deuterostomia (5) or at the base of Bilateria (14). Thus, the evolutionary history of Xenoturbella remains elusive and it is unclear whether its simple body plan represents a plesiomorphic character or, alternatively, is the result of secondary loss (34). In addition, the ecology of Xenoturbella is not well understood, leaving, e.g., its food source unresolved (4, 18).Open in a separate windowFIG. 1.(A) Schematic drawing of the simple body plan of Xenoturbella (redrawn from reference 35). (B) Cells of the gammaproteobacterial endosymbiont (red) detected by probe XenoGam441-CY3 at high magnification (scale bar, 2 μm). (C) Double hybridization of gammaproteobacterial and chlamydial endosymbionts with probes XenoGam441-CY3 (red) and Cps-1353mod-CY5 (displayed in yellow for better contrast, indicated by the arrow); blue-green structures, autofluorescent tissue recorded in the fluorescein isothiocyanate channel (scale bar, 5 μm). (D) FISH detection of gammaproteobacterial endosymbionts with probe XenoGam441-CY3 (red) in a cross section of an X. bocki specimen. The image is composed of five consecutive image stacks, spanning from the gut (to the very left) to the epidermis (to the very right) (magnification, ×400; scale bar, 50 μm). Dense clusters of gammaproteobacterial endosymbionts (red) are confined to the gastrodermis; 4′,6-diamidino-2-phenylindole (DAPI)-stained cell nuclei and mitochondria of X. bocki are displayed in blue; the green and yellow structures are autofluorescent tissue recorded in the fluorescein isothiocyanate channel. (E and F) TEM images of bacterial cells in sections of germinal tissue from a spermatid cluster (E) and of gastric tissue (F) of X. bocki. Arrows point to examples of different putative bacterial morphotypes. Scale bars, 0.5 μm. n, spermatid nucleus; m, spermatid mitochondrion.Symbiotic bacteria are found in a remarkable number of diverse marine invertebrates (11), where they affect both the ecology and the evolution of their hosts. The first indications of intracellular endosymbionts in Xenoturbella were published by Israelsson (17) and confirmed by electron microscopy of a Xenoturbella spermatid cluster (Fig. (Fig.1B);1B); however, our first molecular analyses identified the putative endosymbionts as Gammaproteobacteria, whereas Israelsson (17) had reported a group of endosymbiotic Chlamydiae. The objectives of this study were therefore (i) to identify the putative endosymbionts by 16S rRNA gene analysis, (ii) to localize them inside the host by fluorescence in situ hybridization (FISH), and (iii) to specifically test for the prevalence of the gammaproteobacterial endosymbionts in Xenoturbella. 相似文献
84.
Padmanabhan S Melander O Johnson T Di Blasio AM Lee WK Gentilini D Hastie CE Menni C Monti MC Delles C Laing S Corso B Navis G Kwakernaak AJ van der Harst P Bochud M Maillard M Burnier M Hedner T Kjeldsen S Wahlstrand B Sjögren M Fava C Montagnana M Danese E Torffvit O Hedblad B Snieder H Connell JM Brown M Samani NJ Farrall M Cesana G Mancia G Signorini S Grassi G Eyheramendy S Wichmann HE Laan M Strachan DP Sever P Shields DC Stanton A Vollenweider P Teumer A Völzke H Rettig R Newton-Cheh C 《PLoS genetics》2010,6(10):e1001177
Hypertension is a heritable and major contributor to the global burden of disease. The sum of rare and common genetic variants robustly identified so far explain only 1%–2% of the population variation in BP and hypertension. This suggests the existence of more undiscovered common variants. We conducted a genome-wide association study in 1,621 hypertensive cases and 1,699 controls and follow-up validation analyses in 19,845 cases and 16,541 controls using an extreme case-control design. We identified a locus on chromosome 16 in the 5′ region of Uromodulin (UMOD; rs13333226, combined P value of 3.6×10−11). The minor G allele is associated with a lower risk of hypertension (OR [95%CI]: 0.87 [0.84–0.91]), reduced urinary uromodulin excretion, better renal function; and each copy of the G allele is associated with a 7.7% reduction in risk of CVD events after adjusting for age, sex, BMI, and smoking status (H.R. = 0.923, 95% CI 0.860–0.991; p = 0.027). In a subset of 13,446 individuals with estimated glomerular filtration rate (eGFR) measurements, we show that rs13333226 is independently associated with hypertension (unadjusted for eGFR: 0.89 [0.83–0.96], p = 0.004; after eGFR adjustment: 0.89 [0.83–0.96], p = 0.003). In clinical functional studies, we also consistently show the minor G allele is associated with lower urinary uromodulin excretion. The exclusive expression of uromodulin in the thick portion of the ascending limb of Henle suggests a putative role of this variant in hypertension through an effect on sodium homeostasis. The newly discovered UMOD locus for hypertension has the potential to give new insights into the role of uromodulin in BP regulation and to identify novel drugable targets for reducing cardiovascular risk. 相似文献
85.
Two deltaproteobacterial sulfate reducers, designated strain I.8.1T and I.9.1T, were isolated from the oxygen minimum zone water column off the coast of Peru at 400 and 500 m water depth. The strains were Gram-negative, vibrio-shaped and motile. Both strains were psychrotolerant, grew optimally at 20°C at pH 7.0–8.0 and at 2.5–3.5% NaCl (w/v). The strains grew by utilizing hydrogen/acetate, C3–4 fatty acids, amino acids and glycerol as electron acceptors for sulfate reduction. Fumarate, lactate and pyruvate supported fermentative growth. Sulfate, sulfite, thiosulfate and taurin supported growth as electron acceptors. Both strains were catalase-positive and highly oxygen-tolerant, surviving 24 days of exposure to atmospheric concentrations. MK6 was the only respiratory quinone. The most prominent cellular fatty acid was iso-17:1-ω9c (18%) for strain I.8.1T and iso-17:0-ω9c (14%) for strain I.9.1T. The G+C contents of their genomic DNA were 45–46 mol%. Phylogenetic analysis of 16S rRNA and dsrAB gene sequences showed that both strains belong to the genus Desulfovibrio. Desulfovibrio acrylicus DSM 10141T and Desulfovibrio marinisediminis JCM 14577T represented their closest validly described relatives with pairwise 16S rRNA gene sequence identities of 98–99%. The level of DNA-DNA hybridization between strains I.8.1T and I.9.1T was 30–38%. The two strains shared 10–26% DNA-DNA relatedness with D. acrylicus. Based on a polyphasic investigation it is proposed that strains I.8.1T and I.9.1T represent a novel species for which the name Desulfovibrio oceani sp. nov. is proposed with the two subspecies D. oceani subsp. oceani (type strain, I.8.1T = DSM 21390T = JCM 15970T) and D. oceani subsp. galateae (type strain, I.9.1T = DSM 21391T = JCM 15971T). 相似文献
86.
Ca(2+)-dependent translocation of cytosolic proteins to isolated granule subpopulations and plasma membrane from human neutrophils. 总被引:2,自引:0,他引:2
In order to identify cytosolic proteins involved in control of granule exocytosis in human neutrophils, subcellular fractions enriched in each of the 3 major granule subsets were incubated with cytosol from neutrophils in the presence or absence of Ca2+. After washing, proteins were eluted from the organelles by EGTA. Annexins I, II, IV and VI were found to bind to all organelles studied. In addition, a 28-kDa protein was found to bind exclusively to plasma membranes and secretory vesicles, the most readily exocytosed organelle of neutrophils. Ca(2+)-dependent association of cytosolic proteins to different granule subsets may control differential exocytosis of granules. 相似文献
87.
B. B. Kjellerup K. U. Kjeldsen F. Lopes L. Abildgaard K. Ingvorsen B. Frølund 《Biofouling》2013,29(8):727-737
Severe biofilm formation and biocorrosion have been observed in heating systems even when the water quality complied with existing standards. The coupling between water chemistry, biofilm formation, species composition, and biocorrosion in a heating system was investigated by adding low concentrations of nutrients and oxygen under continuous and alternating dosing regimes. Molecular analysis of 16S rRNA gene fragments demonstrated that the amendments did not cause changes in the overall bacterial community composition. The combined alternating dosing of nutrients and oxygen caused increased rates of pitting (bio-) corrosion. Detection of bacteria involved in sulfide production and oxidation by retrieval of the functional dsrAB and apsA genes revealed the presence of Gram-positive sulfate- and sulfite-reducers and an unknown sulfur-oxidizer. Therefore, to control biocorrosion, sources of oxygen and nutrients must be limited, since the effect of the alternating operational conditions apparently is more important than the presence of potentially corrosive biofilm bacteria. 相似文献
88.
Analysis of a database containing over 20,000 high-resolution collision-activation mass spectra of tryptic peptide dications was employed to study the relative specificity of neutral losses from backbone fragments. The high resolution of the FTMS instrument allowed for the first time the first isotope of the water loss and the monoisotope of the ammonia loss to be distinguished. Contrary to a popular belief, water losses from y' ions are not specific enough to rely upon for detecting the presence of amino acids with oxygen in the side chains. At the same time, ammonia loss from b ions is sufficiently specific (>95%) to detect the presence of amino acids Gln, Asn, His, Lys, and Arg. This feature will be useful for de novo algorithms for high-resolution MS data. Clear trends were observed when the effect of amino acids proximate to the cleavage site on the rate of loss formation was studied. These trends turned out to be different for losses from b and y ions. 相似文献
89.
Anne Marie W. Efsen Anna Schultze Frank A. Post Alexander Panteleev Hansjakob Furrer Robert F. Miller Marcelo H. Losso Javier Toibaro Aliaksandr Skrahin Jose M. Miro Joan A. Caylà Enrico Girardi Mathias Bruyand Niels Obel Daria N. Podlekareva Jens D. Lundgren Amanda Mocroft Ole Kirk TB:HIV study group in EuroCoord 《PloS one》2015,10(12)
Objectives
Rates of TB/HIV coinfection and multi-drug resistant (MDR)-TB are increasing in Eastern Europe (EE). We aimed to study clinical characteristics, factors associated with MDR-TB and predicted activity of empiric anti-TB treatment at time of TB diagnosis among TB/HIV coinfected patients in EE, Western Europe (WE) and Latin America (LA).Design and Methods
Between January 1, 2011, and December 31, 2013, 1413 TB/HIV patients (62 clinics in 19 countries in EE, WE, Southern Europe (SE), and LA) were enrolled.Results
Significant differences were observed between EE (N = 844), WE (N = 152), SE (N = 164), and LA (N = 253) in the proportion of patients with a definite TB diagnosis (47%, 71%, 72% and 40%, p<0.0001), MDR-TB (40%, 5%, 3% and 15%, p<0.0001), and use of combination antiretroviral therapy (cART) (17%, 40%, 44% and 35%, p<0.0001). Injecting drug use (adjusted OR (aOR) = 2.03 (95% CI 1.00–4.09), prior anti-TB treatment (3.42 (1.88–6.22)), and living in EE (7.19 (3.28–15.78)) were associated with MDR-TB. Among 585 patients with drug susceptibility test (DST) results, the empiric (i.e. without knowledge of the DST results) anti-TB treatment included ≥3 active drugs in 66% of participants in EE compared with 90–96% in other regions (p<0.0001).Conclusions
In EE, TB/HIV patients were less likely to receive a definite TB diagnosis, more likely to house MDR-TB and commonly received empiric anti-TB treatment with reduced activity. Improved management of TB/HIV patients in EE requires better access to TB diagnostics including DSTs, empiric anti-TB therapy directed at both susceptible and MDR-TB, and more widespread use of cART. 相似文献90.
Training increases the concentration of [3H]ouabain-binding sites in rat skeletal muscle 总被引:1,自引:0,他引:1
K Kjeldsen E A Richter H Galbo G Lortie T Clausen 《Biochimica et biophysica acta》1986,860(3):708-712
Exercise is associated with a net loss of K+ from the working muscles and an increased plasma K+ concentration, indicating that the capacity for intracellular reaccumulation of K+ is exceeded. Training reduces the exercise-induced rise in plasma K+, and an increased plasma [K+] may interfere with physical performance. Since the clearing of K+ from the extracellular space depends on the capacity for active K+ uptake in skeletal muscle, the effects of training and inactivity on the total concentration of (Na+ + K+)-ATPase was determined. Following 6 weeks of swim training, the concentration of [3H]ouabain-binding sites in rat hindlimb muscles was up to 46% (P less than 0.001) higher than in those obtained from age-matched controls. Whereas muscle Na+, K+ contents remained unchanged, the concentration of citrate synthase increased by up to 76% (P less than 0.001). Training induced no change in the [3H]ouabain-binding-site concentration in the diaphragm, but in the heart ventricles, the K+-dependent 3-O-methylfluorescein phosphatase activity increased by 20% (P less than 0.001). Muscle inactivity induced by denervation, plaster immobilisation or tenotomy reduced the [3H]ouabain-binding-site concentration by 20-30% (P less than 0.02-0.001) within 1 week. In conclusion, training leads to a significant and reversible rise in the concentration of (Na+ + K+)-ATPase in muscle cells. This may be of importance for the beneficial effects on physical performance by improving the maximum capacity for K+ clearance. 相似文献