全文获取类型
收费全文 | 713篇 |
免费 | 34篇 |
出版年
2022年 | 6篇 |
2021年 | 4篇 |
2020年 | 5篇 |
2018年 | 7篇 |
2017年 | 7篇 |
2016年 | 9篇 |
2015年 | 12篇 |
2014年 | 17篇 |
2013年 | 122篇 |
2012年 | 45篇 |
2011年 | 37篇 |
2010年 | 23篇 |
2009年 | 30篇 |
2008年 | 36篇 |
2007年 | 32篇 |
2006年 | 36篇 |
2005年 | 51篇 |
2004年 | 39篇 |
2003年 | 30篇 |
2002年 | 28篇 |
2001年 | 6篇 |
2000年 | 5篇 |
1999年 | 5篇 |
1998年 | 13篇 |
1997年 | 6篇 |
1996年 | 2篇 |
1995年 | 2篇 |
1994年 | 2篇 |
1993年 | 5篇 |
1992年 | 7篇 |
1991年 | 9篇 |
1990年 | 6篇 |
1989年 | 5篇 |
1988年 | 9篇 |
1987年 | 7篇 |
1986年 | 9篇 |
1985年 | 3篇 |
1984年 | 6篇 |
1983年 | 8篇 |
1982年 | 9篇 |
1981年 | 10篇 |
1980年 | 4篇 |
1979年 | 7篇 |
1978年 | 5篇 |
1976年 | 2篇 |
1975年 | 3篇 |
1974年 | 2篇 |
1971年 | 3篇 |
1967年 | 5篇 |
1961年 | 1篇 |
排序方式: 共有747条查询结果,搜索用时 15 毫秒
151.
Yukiko Sasazawa Sanae Souma Norihiko Furuya Yoshiki Miura Saiko Kazuno Soichiro Kakuta Ayami Suzuki Ryota Hashimoto Hiroko HirawakeMogi Yuki Date Masaya Imoto Takashi Ueno Tetsushi Kataura Viktor I Korolchuk Taiji Tsunemi Nobutaka Hattori Shinji Saiki 《The EMBO journal》2022,41(22)
Retrograde transport of lysosomes is recognised as a critical autophagy regulator. Here, we found that acrolein, an aldehyde that is significantly elevated in Parkinson''s disease patient serum, enhances autophagy by promoting lysosomal clustering around the microtubule organising centre via a newly identified JIP4‐TRPML1‐ALG2 pathway. Phosphorylation of JIP4 at T217 by CaMK2G in response to Ca2+ fluxes tightly regulated this system. Increased vulnerability of JIP4 KO cells to acrolein indicated that lysosomal clustering and subsequent autophagy activation served as defence mechanisms against cytotoxicity of acrolein itself. Furthermore, the JIP4‐TRPML1‐ALG2 pathway was also activated by H2O2, indicating that this system acts as a broad mechanism of the oxidative stress response. Conversely, starvation‐induced lysosomal retrograde transport involved both the TMEM55B‐JIP4 and TRPML1‐ALG2 pathways in the absence of the JIP4 phosphorylation. Therefore, the phosphorylation status of JIP4 acts as a switch that controls the signalling pathways of lysosoma l distribution depending on the type of autophagy‐inducing signal. 相似文献
152.
Tadao Asami Nobutaka Takahashi Shigeo Yoshida 《Bioscience, biotechnology, and biochemistry》2013,77(10):2775-2780
A series of 3-(1-alkylaminoalkylidene)-6-methyl-2H-pyran-2,4(3H)-diones was newly synthe-sized, and they were assayed as photosynthetic electron transport (PET) inhibitors because of their structural resemblance to cyanoacrylates and 2-alkylaminoalkylidene-1,3-cyclohexanedione derivatives, which are potent PET inhibitors. Some of the compounds synthesized here showed very high PET inhibition. 相似文献
153.
Possible Mechanism of Dantrolene Stabilization of Cultured Neuroblastoma Cell Plasma Membranes 总被引:2,自引:0,他引:2
Teruo Hayashi Ariyuki Kagaya Nobutaka Motohashi Shigeto Yamawaki 《Journal of neurochemistry》1994,63(5):1849-1854
Abstract: Some reports have suggested that dantrolene interacts directly with the membrane bilayer. We investigated effects of dantrolene on changes in membrane properties induced by compound 48/80 (C48/80), a membrane stimulator. The addition of C48/80 for 1 min elicited a rapid, dose-dependent Ca2+ influx, which was reduced to 14% by the absence of external Ca2+ . Dantrolene inhibited the C48/80-induced increase in Ca2+ permeability of plasma membranes in a concentration-dependent manner (0.33–10 µ M , IC50 value was 5 µ M ). We next examined C48/80-induced changes in structural and dynamic membrane properties by electron spin resonance (ESR). The ratio h 0 / h −1 was determined to evaluate membrane fluidity. C48/80 increased the membrane fluidity in a concentration-dependent manner (0.1–0.56 mg/ml). Dantrolene (10 µ M ) itself did not change the membrane fluidity, but it significantly reduced the C48/80-induced increase in membrane fluidity (0.56 mg/ml). Moreover, the C48/80-induced increase in fluidity was dependent on extracellular Ca2+ . We conclude that dantrolene protects neuroblastoma cell plasma membrane from C48/80-induced membrane perturbation, which causes Ca2+ influx and an increase in membrane fluidity. These findings strongly suggest that dantrolene directly stabilizes the neuronal plasma membrane. 相似文献
154.
155.
Shozo Fujioka Akira Sakurai Naotsugu Sugimoto Isomaro Yamaguchi Noboru Murofushi Nobutaka Takahashi 《Bioscience, biotechnology, and biochemistry》2013,77(8):2053-2059
Efforts were made to isolate flower-inhibitory substances from extracts of the short-day plant Lemna paucicostata 381. Lemna paucicostata 151, which was used in the bioassay, exhibits poor flowering in response to the photoperiod, but flowers profusely in response to benzoic acid. Therefore, only those substances that inhibit benzoic acid-induced flowering were studied. Several fractions obtained by silica gel column chromatography exhibited flower-inhibitory activity when tested on L. paucicostata 151. After several purification steps, one of the active principles was identified as lutein by MS, UV and NMR spectroscopic analyses. Lutein and its isomer zeaxanthin inhibited benzoic acid-induced flowering in both L. paucicostata 151 and 381. 相似文献
156.
Izumi Kazuo; Kamiya Yuji; Sakurai Akira; Oshio Hiromichi; Takahashi Nobutaka 《Plant & cell physiology》1985,26(5):821-827
(E)-1-(4-Chlorophenyl)-4,4-dimethyl-2-(1,2,4-triazol-1-yl)-1-penten-3-ol(S-3307), a new plant growth retardant, was investigated todetermine the inhibitory sites in gibberellin biosynthesis andthe comparative effects of its stereoisomers in a cell-freesystem from Cucurbita maxima. After treatment with S-3307, theincorporation of [14C]mevalonic acid into GA12-aldehyde andGA12 clearly was inhibited, and kaurene accumulated. Feedingexperiments showed that S-3307 inhibited the oxidation of kaurene,kaurenol and kaurenal, but did not affect the oxidation of kaurenoicacid. Thus the reaction sites of S-3307 in gibberellin biosynthesiswere shown to be the three oxidation steps from kaurene to kaurenoicacid. Because S-3307 has an asymmetric center and a tri-substituteddouble bond, there are four stereoisomers; two optical isomersand two geometrical isomers. The activities of these isomersas a growth retardant and gibberellin biosynthesis inhibitorwere examined with a rice seedling assay and the cell-free system.The relative activity of these isomers was basically the samein the two assay methods. The (S)-(E) form was the most active,being 7-fold more active in the cell-free system and 70-foldmore active in the bioassay than the (R)-(E) form. The (RS)-(Z)form showed no activity in the cell-free system, but weak dwarfingeffects in the rice seedling assay. (Received January 19, 1985; Accepted April 13, 1985) 相似文献
157.
Kwak Sang-Soo; Kamiya Yuji; Takahashi Masahiro; Sakurai Akira; Takahashi Nobutaka 《Plant & cell physiology》1988,29(4):707-711
The metabolism of [14C]GA20 during seed maturation of Phaseolusvulgaris was studied using cell-free systems from embryos rangingin age from 10 DAF (day after flowering) to 24 DAF. Enzyme preparationsfrom very immature seeds actively converted GA20 to GA1, GA5and GA6. The ratio of incubation products suggested the biosyntheticpathway of GA20GA5GA6. Fluctuation in the levelsof endogenous C19-GAs, namely GA1, GA4, GA5, GA6, GA8, GA9 andGA20 was analyzed by GC-SIM using internal standards to compareenzyme activity with the levels of endogenous GAs. AlthoughGA1, GA4 and GA6 showed maximum levels on 20 DAF, enzyme activitydecreased during seed maturation and showed weak activity on20 DAF.
1Graduate student of the University of Tokyo, Department ofAgricultural Chemistry, Bunkyo-ku, Tokyo 113, Japan.
3Present address: Pesticides Research Laboratory, TakarazukaResearch Center, Sumitomo Chemical Co., Ltd., Takarazuka, Hyogo655, Japan. (Received December 17, 1987; Accepted March 30, 1988) 相似文献
158.
Kwak Sang-Soo; Kamiya Yuji; Sakurai Akira; Takahashi Nobutaka; Graebe Jan E. 《Plant & cell physiology》1988,29(6):935-943
Gibberellin 3/ß-hydroxylase,a 2-oxoglutarate-dependentdioxygenase that catalyzes the hydroxylation of GA20 to GA1,was purified 313-fold from immature seeds of Phaseolus vulgarisL. The mol wt of the enzyme was estimated to be 42,000 by gelfiltration HPLC and SDS-polyacrylamide gel electrophoresis.The enzyme exhibited maximum activity at pH 7.7. The Km valuesfor [2,3-3H]GA20 and [2,3-3H]GA, were 0.29µu and 0.33µm, respectively. The enzyme requires 2-oxoglutarate asa cosubstrate; the Km value for 2-oxoglutarate was 250µMusing [3H]- GA20 as a substrate. Fe2+ and ascorbate significantlyactivated the enzyme at all purification steps, while catalaseand BSA activated the purified enzyme only. The enzyme was inhibitedby divalent cations Mn2+, Co2+, Ni2+, Cu2+, Zn2+, Cd2+ and Hg2+.3ß-Hydroxylation of [3H]- GA20 was also inhibitedby non-radioactive GA5, GA9,GA15, GA20 and GA44. The possiblesite of 3ß-hydroxylation in gibberellin biosynthesisis discussed in terms of the substrate specificity of partiallypurified gibberellin 3ß-hydroxylase. (Received February 29, 1988; Accepted June 3, 1988) 相似文献
159.
Issay Narumi Kazumi Sawakami Shinya Nakamoto Noriyuki Nakayama Tadashi Yanagisawa Nobutaka Takahashi Hiroshi Kihara 《Biotechnology Techniques》1992,6(1):83-86
Summary Optimal conditions for the plasmid transformation of a newly isolatedBacillus
stearothermophilus K1041 by electroporation were investigated. The optimal conditions allowed a transformation efficiency of 5.8×105 transformants per μg plasmid pUB110. 相似文献
160.